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Open AccessArticle

Development of a Microfluidic Array to Study Drug Response in Breast Cancer

1
Department of Biomedical Engineering, University of Wisconsin-Madison, 1550 Engineering Drive, Madison, WI 53706, USA
2
The University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA
3
School of Medicine and Public Health, University of Wisconsin-Madison, 750 Highland Avenue, Madison, WI 53726, USA
4
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706, USA
5
Morgridge Institute for Research, 330 N Orchard street, Madison, WI 53715, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: R. Michael Van Dam
Molecules 2019, 24(23), 4385; https://doi.org/10.3390/molecules24234385
Received: 4 November 2019 / Revised: 25 November 2019 / Accepted: 26 November 2019 / Published: 30 November 2019
(This article belongs to the Special Issue Microfluidic Tools for High-Throughput Screening)
Luminal geometries are common structures in biology, which are challenging to mimic using conventional in vitro techniques based on the use of Petri dishes. In this context, microfluidic systems can mimic the lumen geometry, enabling a large variety of studies. However, most microfluidic models still rely on polydimethylsiloxane (PDMS), a material that is not amenable for high-throughput fabrication and presents some limitations compared with other materials such as polystyrene. Thus, we have developed a microfluidic device array to generate multiple bio-relevant luminal structures utilizing polystyrene and micro-milling. This platform offers a scalable alternative to conventional microfluidic devices designed in PDMS. Additionally, the use of polystyrene has well described advantages, such as lower permeability to hydrophobic molecules compared with PDMS, while maintaining excellent viability and optical properties. Breast cancer cells cultured in the devices exhibited high cell viability similar to PDMS-based microdevices. Further, co-culture experiments with different breast cell types showed the potential of the model to study breast cancer invasion. Finally, we demonstrated the potential of the microfluidic array for drug screening, testing chemotherapy drugs and photodynamic therapy agents for breast cancer. View Full-Text
Keywords: polystyrene; microfluidics; lumen polystyrene; microfluidics; lumen
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Virumbrales-Muñoz, M.; Livingston, M.K.; Farooqui, M.; Skala, M.C.; Beebe, D.J.; Ayuso, J.M. Development of a Microfluidic Array to Study Drug Response in Breast Cancer. Molecules 2019, 24, 4385.

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