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Microfluidic Technologies for High Throughput Screening Through Sorting and On-Chip Culture of C. elegans

Department of Chemical & Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA
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Author to whom correspondence should be addressed.
Academic Editor: R. Michael Van Dam
Molecules 2019, 24(23), 4292; https://doi.org/10.3390/molecules24234292
Received: 2 November 2019 / Revised: 20 November 2019 / Accepted: 22 November 2019 / Published: 25 November 2019
(This article belongs to the Special Issue Microfluidic Tools for High-Throughput Screening)
The nematode Caenorhabditis elegans is a powerful model organism that has been widely used to study molecular biology, cell development, neurobiology, and aging. Despite their use for the past several decades, the conventional techniques for growth, imaging, and behavioral analysis of C. elegans can be cumbersome, and acquiring large data sets in a high-throughput manner can be challenging. Developments in microfluidic “lab-on-a-chip” technologies have improved studies of C. elegans by increasing experimental control and throughput. Microfluidic features such as on-chip control layers, immobilization channels, and chamber arrays have been incorporated to develop increasingly complex platforms that make experimental techniques more powerful. Genetic and chemical screens are performed on C. elegans to determine gene function and phenotypic outcomes of perturbations, to test the effect that chemicals have on health and behavior, and to find drug candidates. In this review, we will discuss microfluidic technologies that have been used to increase the throughput of genetic and chemical screens in C. elegans. We will discuss screens for neurobiology, aging, development, behavior, and many other biological processes. We will also discuss robotic technologies that assist in microfluidic screens, as well as alternate platforms that perform functions similar to microfluidics. View Full-Text
Keywords: screening; microfluidics; phenotyping; genetics; aging screening; microfluidics; phenotyping; genetics; aging
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Midkiff, D.; San-Miguel, A. Microfluidic Technologies for High Throughput Screening Through Sorting and On-Chip Culture of C. elegans. Molecules 2019, 24, 4292.

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