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Health Promoting Compounds in Milk and Dairy Products, 2nd Edition

A Special Issue of Molecules (ISSN 1420-3049) belonging to the section "Food Chemistry".

Deadline for manuscript submissions: 30 April 2027 | Viewed by 4423

Editors


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Guest Editor
CREA—Consiglio per la Ricerca in Agricoltura e l’Analisi Dell’Economia Agraria Centro di Ricerca Alimenti e Nutrizione, Via Ardeatina 546, 00178 Rome, Italy
Interests: analytical chemistry; food science; sample preparation; chromatography; spectroscopy; multivariate data analysis
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
CREA—Council for Agricultural Research and Economics, Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy
Interests: analytical chemistry; food science; sample preparation; chromatography; spectroscopy; multivariate data analysis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

You are invited to contribute to a new edition of a Special Issue on milk and dairy products, entitled "Health Promoting Compounds in Milk and Dairy Products, 2nd Edition".

Milk is an essential component of the human diet. It contains all the substances necessary for the growth of an organism and it can be considered a balanced source of proteins with high biological value, sugars, fats, minerals, and vitamins. Milk can be consumed as is or in the form of yoghurts, cheeses, or several other dairy products. In addition to their excellent nutritional value and good sensory properties, milk and dairy products are also considered to be a good source of functional compounds. The numerous research works in the field have confirmed the nutritional importance of dairy products in the prevention of several chronic diseases, and the benefits of consuming dairy products, attributed to the presence of several bioactive compounds (e.g., bioactive peptides, fatty acids such as conjugated linoleic acid and odd-chain fatty acids, antioxidant compounds, vitamins, and minerals), are also well known.

The quality of milk and dairy products, as well as their content of bioactive compounds, can be influenced by several factors, such as the animal genetics, the farming techniques, the animal diet (e.g., more or less rich in fresh grass), the technological processes (e.g., heat treatments, fermentation processes, or the addition of specific ingredients rich in bioactive compounds), and so on. Recently, new technologies such as nanotechnology and non-thermal technologies have been developed to provide new solutions for including bioactive compounds in dairy products, improving the functional properties of these food items without affecting consumers’ sensory perception.

This Special Issue "Health Promoting Compounds in Milk and Dairy Products, 2nd Edition" aims to investigate not only the analytical methods for assessing the bioactive compounds in various dairy products, but also to update the information about the nutritional evaluation of milk-based food products. Another aim of this Special Issue is to collect recent studies focused on the improvement of the nutritional value of milk and dairy products due to the breeding and/or feeding conditions, and the role of the technological processes in keeping or enhancing the bioactive compounds in milk-based food products. Contributions on the effects of the bioactive compounds in milk and dairy products in the human diet are also welcome, as are manuscripts addressing the assessment of these functional compounds in promoting health.

Dr. Pamela Manzi
Dr. Mena Ritota
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • milk
  • dairy products
  • functional compounds
  • food quality
  • protein and bioactive peptides
  • minerals
  • vitamins
  • antioxidant compounds
  • promoting health and well-being

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Related Special Issue

Published Papers (3 papers)

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Research

23 pages, 1085 KB  
Article
Effects of Prepartum Supplementation with Flaxseed and Black Soldier Fly Larvae Oils on Fatty Acid Composition and Lipid Quality Indices in Sheep Milk
by Kamila Lewandowska, Natalia Pachura-Hanusek, Antoni Szumny, Anna Zielak-Steciwko and Robert Kupczyński
Molecules 2026, 31(15), 2628; https://doi.org/10.3390/molecules31152628 - 28 Jul 2026
Viewed by 435
Abstract
The peripartum period in prolific ewes is associated with increased metabolic demands, and dietary lipid supplementation may increase the energy density of the prepartum diet. This study evaluated the effects of prepartum supplementation with alternative lipid matrices on the physicochemical characteristics and comprehensive [...] Read more.
The peripartum period in prolific ewes is associated with increased metabolic demands, and dietary lipid supplementation may increase the energy density of the prepartum diet. This study evaluated the effects of prepartum supplementation with alternative lipid matrices on the physicochemical characteristics and comprehensive fatty acid (FA) profiles of mature and transitional sheep milk. Thirty-two pregnant Olkuska ewes were randomly assigned to four cohorts (n = 8/group): Control (basal diet), flaxseed oil (low n-3/high n-6 variety; FO), black soldier fly larvae oil (BSFL oil), and Mix oil (2:1 ratio of FO to BSFL oil). Dietary treatments exerted no significant effects (p > 0.05) on transitional milk composition or lipid quality indices. In contrast, in mature milk, BSFL oil supplementation significantly increased milk fat content (6.09%) compared to the Control group (4.70%; p < 0.01). Furthermore, all oil treatments significantly reduced total saturated FAs (p < 0.01), while the BSFL oil and Mix oil supplementation significantly increased the monounsaturated FA fraction (p < 0.01). The differences, particularly the increase in cis-9, trans-11 CLA concentration and the significant reduction in both the atherogenic and thrombogenic indices (p < 0.01), were observed exclusively in mature milk. These findings suggest that the combination of sustainable insect lipids with high-linoleic plant oils may represent a viable strategy to transform sheep milk into a functional food with potential cardiovascular health benefits for consumers. These findings additionally indicate that prepartum lipid supplementation may exert a persistent carry-over effect into early lactation, influencing the fatty acid composition of mature sheep milk. Full article
(This article belongs to the Special Issue Health Promoting Compounds in Milk and Dairy Products, 2nd Edition)
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12 pages, 6639 KB  
Article
Comprehensive Analysis of Exosomal microRNAs in Buffalo Milk Across the Early Postpartum Transition
by Jiazheng Zhu, Rongchun Huang, Pingbai Liu, Yuan Yang, Yue Zhang, Shengfei Yan, Gan Liang, Meiting Chen, Mengyuan Zhou, Guangsheng Qin and Qiang Fu
Molecules 2026, 31(8), 1332; https://doi.org/10.3390/molecules31081332 - 18 Apr 2026
Cited by 1 | Viewed by 717
Abstract
Milk-derived exosomes (MDEs) are bioactive nanocarriers rich in microRNAs (miRNAs) that play critical roles in post-transcriptional regulation during neonatal development and immune adaptation. However, the dynamic changes in miRNA expression across lactation stages and their biological functions remain insufficiently explored. We hypothesized that [...] Read more.
Milk-derived exosomes (MDEs) are bioactive nanocarriers rich in microRNAs (miRNAs) that play critical roles in post-transcriptional regulation during neonatal development and immune adaptation. However, the dynamic changes in miRNA expression across lactation stages and their biological functions remain insufficiently explored. We hypothesized that the miRNA cargo of buffalo MDEs exhibits temporal specificity, thereby dynamically matching the immune requirements of the neonatal calves. Therefore, the present study aimed to systematically characterize the miRNA expression profiles of MDEs derived from colostrum, transitional milk, and mature milk. MDEs were isolated, purified using differential ultracentrifugation, and characterized via transmission electron microscopy, Western blotting, and nanoparticle-tracking analysis. A total of 370 miRNAs were identified in the MDEs, with 220 (59.5%) co-expressed across colostrum, transitional milk, and mature milk. Comparative analysis revealed that colostrum MDEs exhibited the greatest miRNA diversity. Expression patterns of miRNAs showed distinct stage-specific clustering as lactation progressed. Compared to mature milk, 100 differentially expressed miRNAs (DE-miRNAs) were identified in colostrum MDEs, including 39 upregulated and 61 downregulated miRNAs. Bioinformatics analyses indicated that predicted target genes were associated with transmembrane transport, immune response, cell development, and apoptosis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified pathways involved in immune regulation, inflammation, and apoptosis. Moreover, macrophages incubated with buffalo colostrum MDEs showed upregulation of proliferation-related genes and downregulation of pro-inflammatory factors, suggesting an anti-inflammatory effect through activation of the phosphoinositide 3-kinase-protein kinase B (PI3K-Akt) signaling pathway. These findings offer new insights into miRNA profiles of buffalo MDEs across the early postpartum transition and provide a preliminary basis for exploring immunomodulatory potential of buffalo MDEs. Full article
(This article belongs to the Special Issue Health Promoting Compounds in Milk and Dairy Products, 2nd Edition)
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22 pages, 2379 KB  
Article
Release of Bioactive Peptides from Whey Protein During In Vitro Digestion and Their Effect on CCK Secretion in Enteroendocrine Cells: An In Silico and In Vitro Approach
by Anaís Ignot-Gutiérrez, Orlando Arellano-Castillo, Gloricel Serena-Romero, Mayvi Alvarado-Olivarez, Daniel Guajardo-Flores, Armando J. Martínez and Elvia Cruz-Huerta
Molecules 2026, 31(2), 238; https://doi.org/10.3390/molecules31020238 - 10 Jan 2026
Cited by 2 | Viewed by 2606
Abstract
During gastrointestinal digestion, dietary proteins are hydrolyzed into peptides and free amino acids that modulate enteroendocrine function and satiety-related hormone secretion along the gut–brain axis, thereby contributing to obesity prevention. We investigated whey protein concentrate (WPC) as a source of bioactive peptides and [...] Read more.
During gastrointestinal digestion, dietary proteins are hydrolyzed into peptides and free amino acids that modulate enteroendocrine function and satiety-related hormone secretion along the gut–brain axis, thereby contributing to obesity prevention. We investigated whey protein concentrate (WPC) as a source of bioactive peptides and evaluated the effects of its digests on cholecystokinin (CCK) secretion in STC-1 enteroendocrine cells by integrating the standardized INFOGEST in vitro digestion protocol, peptidomics (LC–MS/MS), and in silico bioactivity prediction. In STC-1 cells, the <3 kDa intestinal peptide fraction exhibited the strongest CCK stimulation, positioning these low-molecular-weight peptides as promising bioactive components for satiety modulation and metabolic health applications. Peptidomic analysis of this fraction identified short sequences derived primarily from β-lactoglobulin (β-La) and α-lactalbumin (α-La), enriched in hydrophobic and aromatic residues, including neuropeptide-like sequences containing the Glu–Asn–Ser–Ala–Glu–Pro–Glu (ENSAEPE) motif of β-La f(108–114). In silico bioactivity profiling with MultiPep predicted antihypertensive, angiotensin-converting enzyme (ACE)–inhibitory, antidiabetic, dipeptidyl peptidase-IV (DPP-IV)–inhibitory, antioxidant, antibacterial, and neuropeptide-like activities. Overall, digestion of WPC released low-molecular-weight peptides and amino acids that enhanced CCK secretion in vitro; these findings support their potential use in nutritional strategies to enhance satiety, modulate appetite and energy intake, and improving cardiometabolic health. Full article
(This article belongs to the Special Issue Health Promoting Compounds in Milk and Dairy Products, 2nd Edition)
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