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Special Issue "Analytical Methods for Mycotoxin Analysis"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 May 2020.

Special Issue Editors

Prof. Dr. Terenzio Bertuzzi
E-Mail Website
Guest Editor
Dipartimento di Scienze animali, della nutrizione e degli alimenti (DIANA) Università Cattolica del Sacro Cuore, Milan, Italy
Interests: development of analytical methods for mycotoxin determination in food and feed
Dr. Chiara Lanzanova
E-Mail Website
Guest Editor
CREA Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Centro di ricerca Cerealicoltura e Colture Industriali
Interests: fungal pathogens; mycotoxins; crop protection
Dr. Sabrina Locatelli
E-Mail Website
Guest Editor
CREA Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia agrarian, Centro di ricerca Cerealicoltura e Colture Industriali
Interests: mycotoxins; crops; safety

Special Issue Information

Dear Colleagues,

Mycotoxins are secondary metabolites produced by fungi of different species, mainly Aspergillus, Fusarium, Penicillium, and Alternaria, which can contaminate food and feed with toxic effects for humans and animals. They represent the most important class of chemical hazards recorded in the European Rapid Alert System on Feed and Food (RASFF). In 2017, 43.4% of the alerts concerning a potential threat to human health from chemical contaminants in food products, mainly cereals and nuts, were due to mycotoxins.

Regulations around the world have established maximum levels for different mycotoxins in foodstuffs, including aflatoxins B1, B2, G1, G2, and M1, ochratoxin A, fumonisins B1 and B2, deoxynivalenol, zearalenone, HT-2 and T-2 toxins, patulin, citrinin, and ergot alkaloids. In addition to these “known and legislatively regulated” mycotoxins, there are other “emerging mycotoxins” that have been considered as relevant since evidence of their incidence in food and feed is rapidly increasing. This group includes Alternaria toxins, such as alternariol, alternariol monomethyl ether, and tenuazonic acid, Aspergillus toxins, such as sterigmatocystin, Fusarium toxins, such as moniliformin, enniantins and beauvericin, phomopsins, and others.

Therefore, it is necessary to develop analytical methods for an accurate determination of mycotoxins in different food matrices and feeds, achieving both the requirement of assuring food safety along the production chain and the development of advanced instrumental techniques to identify new emerging and masked mycotoxins. To obtain a continuous monitoring of these hazardous compounds in raw materials and final products along the production food chain, rapid, cheap, and easy-to-operate analytical methods are generally used; on the other hand, innovative approaches and advanced instrumental techniques, such as GC-MS, LC-MS/MS and HRMS, are required to develop multi-mycotoxin methods and to identify and quantify emerging, masked, and novel mycotoxins. Moreover, the high variety of samples involved in mycotoxin determination requires the development of appropriate extraction and clean-up techniques.

I hope that this Special Issue of Molecules will contribute to the advancement of analytical methods helpful in evaluating mycotoxin occurrence and diffusion in food and feed.

Prof. Dr. Terenzio Bertuzzi
Dr. Chiara Lanzanova
Dr. Sabrina Locatelli
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Mycotoxins
  • Analytical methods
  • Food safety
  • Monitoring

Published Papers (1 paper)

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Open AccessArticle
Identification and Distribution of Novel Metabolites of Lolitrem B in Mice by High-Resolution Mass Spectrometry
Molecules 2020, 25(2), 372; - 16 Jan 2020
Lolitrem B is the most potent indole-diterpene mycotoxin produced by Epichloë festucae var. lolii (termed LpTG-1), with severe intoxication cases reported in livestock. To date, there are no in vivo metabolism studies conducted for the mycotoxin. A mouse model assay established for [...] Read more.
Lolitrem B is the most potent indole-diterpene mycotoxin produced by Epichloë festucae var. lolii (termed LpTG-1), with severe intoxication cases reported in livestock. To date, there are no in vivo metabolism studies conducted for the mycotoxin. A mouse model assay established for assessing toxicity of indole-diterpenes was used to investigate metabolic products of lolitrem B. Mice were administered lolitrem B at 0.5 and 2.0 mg/kg body weight (b.wt) intraperitoneally before body and brain tissues were collected at 6 h and 24 h post-treatment. Samples were cryoground and subjected to a biphasic or monophasic extraction. The aqueous and lipophilic phases were analysed using liquid chromatography high-resolution mass spectrometry (LC–HRMS); data analysis was performed with Compound Discoverer™ software. A total of 10 novel phase I metabolic products were identified in the lipophilic phase and their distribution in the liver, kidney and various brain regions are described. The biotransformation products of lolitrem B were found to be present in low levels in the brain. Based on structure–activity postulations, six of these may contribute towards the protracted tremors exhibited by lolitrem B-exposed animals. Full article
(This article belongs to the Special Issue Analytical Methods for Mycotoxin Analysis)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

planned paper by Prof. Dr. Franz Berthiller

Title: Development and validation of a LC-MS/MS based method for the determination of deoxynivalenol and its modified forms in cereals

Abstract: In 2017, EFSA set a group-TDI of 1 µg/kg bw for the sum of deoxynivalenol, its 3- and 15-acetylated forms as well as 3-glucoside. Somehow surprisingly, their accurate determination is still hampered by a number of challenges. First of all, the acetylated forms cannot be separated under typical HPLC conditions and 13C-labelled DON-3-glucoside was not available as an internal standard until we produced it recently. We (almost) solved both problems and developed and validated the first stable isotope
dilution LC-MS/MS method for their concurrent determination. The optimised method with a run time of 7 minutes almost base-line separates the critical pair of 3- and 15-AcDON and very nicely compensates for all matrix effects in maize and wheat.

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