molecules-logo

Journal Browser

Journal Browser

New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics, 2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 30 September 2026 | Viewed by 925

Editors


E-Mail Website
Guest Editor
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
Interests: natural deep eutectic solvents; green extractions; phytochemistry; ethnopharmacology; interaction between environmental stress and secondary metabolites in plants
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
1. Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
2. Department of Plant Physiology and Biophysics, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, 19 Akademicka St., 20-033 Lublin, Poland
Interests: phytochemistry; pharmacognosy; plant–environment interactions; elicitation of secondary metabolites; chemoinformatics in phytochemistry
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The extraction of secondary metabolites, including flavonoids, phenolics, glycosides, terpene compounds, or nitrogenous compounds, from plants is one of the most important steps in phytochemical research. Currently, numerous extraction methods and techniques are used; however, new approaches are constantly being developed to meet evolving requirements and expectations. Modern approaches to the extraction of plant material target are targeted only at the minimization of costs, but also at simplicity, flexibility, and care for the natural environment. Process miniaturization is also important, as it allows the analysis of very small samples. The researchers’ attention is therefore focused on the use of micro- and nano-extraction, limiting the consumption of toxic extractants through the appropriate design of experiments (DOEs) and using non-toxic extractants such as supercritical carbon dioxide or natural deep eutectic solvents (NADES).

This Special Issue of Molecules will focus on new approaches to the extraction of biologically active molecules from plants and, in this context, the chemical and physical interactions between molecules and extracted constituents (liquid phase, solid phase, and gas phase). We welcome research and review articles that contribute to the following topics, among others:

  • Utilization of computation techniques in extraction (DOE);
  • NADES (natural deep eutectic solvents);
  • New equipment and techniques for extraction processes;
  • Micro- and nano-extraction;
  • Supercritical fluid extraction;
  • Enzyme-assisted extraction;
  • Theoretical background of “green extraction”.

Dr. Maciej Strzemski
Prof. Dr. Sławomir Dresler
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant secondary metabolites
  • biologically active compounds
  • design of experiments
  • natural deep eutectic solvents
  • ionic liquids
  • micro- and nano-solid phase extraction
  • accelerated solvent extraction
  • supercritical fluid extraction
  • single-drop extraction

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Related Special Issue

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

28 pages, 6652 KB  
Article
Volatile Natural Deep Eutectic Solvents (VNADESs) for Extraction of Shikonin Derivatives from Echium vulgare Roots and Evaluation of Biological Activity
by Magdalena Kulinowska, Sławomir Dresler, Agnieszka Grzegorczyk, Martyna Zagórska-Dziok, Aleksandra Ziemlewska, Wirginia Kukula-Koch, Katarzyna Sawa-Wejksza and Maciej Strzemski
Molecules 2026, 31(9), 1434; https://doi.org/10.3390/molecules31091434 - 26 Apr 2026
Viewed by 542
Abstract
Background: Shikonins are natural naphthoquinones that exhibit a range of biological activities. They are typically extracted using nonpolar solvents; however, green extraction approaches remain underexplored. Methods: Phytochemical profiling of E. vulgare root extracts was performed using HPLC-ESI-QTOF-MS/MS and quantitative analysis using HPLC-PDA. Shikonin [...] Read more.
Background: Shikonins are natural naphthoquinones that exhibit a range of biological activities. They are typically extracted using nonpolar solvents; however, green extraction approaches remain underexplored. Methods: Phytochemical profiling of E. vulgare root extracts was performed using HPLC-ESI-QTOF-MS/MS and quantitative analysis using HPLC-PDA. Shikonin extraction was performed using VNADESs based on thymol, camphor, menthol and benzyl alcohol. The feasibility of removing the VNADES from the extracts via freeze-drying was assessed. The cytotoxic, antioxidant, anti-inflammatory and antimicrobial activities of the hexane extract and the selected VNADES-based extract (TBa 2:8) were compared. Results: Eight shikonin derivatives were identified in the extracts. VNADES extracts contained comparable amounts of shikonin to hexane extracts; however, freeze-drying resulted in significant shikonin content loss. TBa 2:8 extract exhibited noticeably lower cytotoxicity than the hexane extract while its antioxidant potential depended on the assay applied. In contrast to the hexane extract, TBa 2:8 demonstrated the ability to reduce intracellular ROS and NO levels. However, the hexane extract exhibited stronger antimicrobial activity. Conclusions: VNADES systems enable efficient extraction of shikonin derivatives with performance comparable to hexane. Although the resulting extracts exhibit multidirectional biological activity, it remains challenging to remove the VNADESs effectively without losing the shikonins. Full article
Show Figures

Figure 1

Back to TopTop