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Microorganisms 2018, 6(2), 42; https://doi.org/10.3390/microorganisms6020042

Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae

Department of Biotechnology and Food Science, NTNU: Norwegian University of Science and Technology, 7491 Trondheim, Norway
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Received: 16 April 2018 / Revised: 2 May 2018 / Accepted: 3 May 2018 / Published: 10 May 2018
(This article belongs to the Special Issue Recombinant Protein Expression in Microorganisms)
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Abstract

Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins. In particular, for thermostable proteins, there is a need for hosts able to properly synthesize, fold, and excrete these in high yields, and thermophilic Bacillaceae represent one potentially interesting group of microorganisms for such purposes. A number of thermophilic Bacillaceae including B. methanolicus, B. coagulans, B. smithii, B. licheniformis, Geobacillus thermoglucosidasius, G. kaustophilus, and G. stearothermophilus are investigated concerning physiology, genomics, genetic tools, and technologies, altogether paving the way for their utilization as hosts for recombinant production of thermostable and other difficult-to-express proteins. Moreover, recent successful deployments of CRISPR/Cas9 in several of these species have accelerated the progress in their metabolic engineering, which should increase their attractiveness for future industrial-scale production of proteins. This review describes the biology of thermophilic Bacillaceae and in particular focuses on genetic tools and methods enabling use of these organisms as hosts for recombinant protein production. View Full-Text
Keywords: recombinant expression; thermophiles; Bacillus; Geobacillus; Bacillaceae recombinant expression; thermophiles; Bacillus; Geobacillus; Bacillaceae
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Drejer, E.B.; Hakvåg, S.; Irla, M.; Brautaset, T. Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae. Microorganisms 2018, 6, 42.

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