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Review

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

by 1,2 and 3,*
1
Center of Food and Fermentation Technologies, Akadeemia tee 15a, 12618 Tallinn, Estonia
2
Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia
3
Department of Bioprocess Engineering, Technische Universität Berlin, Ackerstraβe 76, ACK24, D-13355 Berlin, Germany
*
Author to whom correspondence should be addressed.
Microorganisms 2018, 6(3), 56; https://doi.org/10.3390/microorganisms6030056
Received: 20 April 2018 / Revised: 28 May 2018 / Accepted: 19 June 2018 / Published: 21 June 2018
(This article belongs to the Special Issue Recombinant Protein Expression in Microorganisms)
Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate of the culture is advantageous. Continuous culture methods were developed to grow cells in a constant environment and have been used for decades to study basic microbial physiology in a controlled and reproducible manner. Our review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms. View Full-Text
Keywords: continuous cultures; continuous manufacturing; recombinant protein production; steady state; specific growth rate; chemostat continuous cultures; continuous manufacturing; recombinant protein production; steady state; specific growth rate; chemostat
MDPI and ACS Style

Peebo, K.; Neubauer, P. Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms. Microorganisms 2018, 6, 56. https://doi.org/10.3390/microorganisms6030056

AMA Style

Peebo K, Neubauer P. Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms. Microorganisms. 2018; 6(3):56. https://doi.org/10.3390/microorganisms6030056

Chicago/Turabian Style

Peebo, Karl, and Peter Neubauer. 2018. "Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms" Microorganisms 6, no. 3: 56. https://doi.org/10.3390/microorganisms6030056

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