Next Article in Journal
Potential Role for the Gut Microbiota in Modulating Host Circadian Rhythms and Metabolic Health
Next Article in Special Issue
ATP- and Polyphosphate-Dependent Glucokinases from Aerobic Methanotrophs
Previous Article in Journal
Candida glabrata: A Lot More Than Meets the Eye
Previous Article in Special Issue
An Alternative Platform for Protein Expression Using an Innate Whole Expression Module from Metagenomic DNA
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessReview
Microorganisms 2019, 7(2), 40; https://doi.org/10.3390/microorganisms7020040

Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica

TERRA Teaching and Research Centre, Microbial Processes and Interactions, University of Liège–Gembloux AgroBio Tech, 5030 Gembloux, Belgium
*
Author to whom correspondence should be addressed.
Received: 17 January 2019 / Revised: 28 January 2019 / Accepted: 29 January 2019 / Published: 30 January 2019
(This article belongs to the Special Issue Recombinant Protein Expression in Microorganisms)
Full-Text   |   PDF [780 KB, uploaded 30 January 2019]   |  

Abstract

Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possibility of post-translational modifications. Yarrowia lipolytica is considered as one of the most attractive hosts due to its ability to metabolize raw substrate, to express genes at a high level, and to secrete protein in large amounts. In recent years, several reviews have been dedicated to genetic tools developed for this purpose. Though the construction of efficient cell factories for recombinant protein synthesis is important, the development of an efficient process for recombinant protein production in a bioreactor constitutes an equally vital aspect. Indeed, a sports car cannot drive fast on a gravel road. The aim of this review is to provide a comprehensive snapshot of process tools to consider for recombinant protein production in bioreactor using Y. lipolytica as a cell factory, in order to facilitate the decision-making for future strain and process engineering. View Full-Text
Keywords: Yarrowia lipolytica; recombinant protein; bioreactor; metabolic load Yarrowia lipolytica; recombinant protein; bioreactor; metabolic load
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Vandermies, M.; Fickers, P. Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica. Microorganisms 2019, 7, 40.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top