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Microorganisms
Special Issues
Advanced Research on Antimicrobial Activity of Natural Products
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Advanced Research on Antimicrobial Activity of Natural Products

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Antimicrobial Agents and Resistance".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1710

Special Issue Editors

Dr. Anca Zanfirescu

E-Mail Website
Guest Editor
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 020956 Bucharest, Romania
Interests: AI in drug design; small-molecule therapeutics; drug target prediction; pharmacoinformatics
Special Issues, Collections and Topics in MDPI journals
Dr. Corina Andrei

E-Mail Website
Guest Editor Assistant
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 020956 Bucharest, Romania
Interests: computational drug discovery; pharmacoinformatics; virtual screening
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The global rise of antimicrobial resistance presents a critical challenge to public health, as antibiotics steadily lose their effectiveness against a growing number of resistant pathogens. This Special Issue calls for innovative solutions, and natural products have emerged as promising candidates for the development of novel antimicrobial agents.

We are delighted to announce this Special Issue, titled “Advanced Research on Antimicrobial Activity of Natural Products”, dedicated to showcasing groundbreaking advancements in this dynamic field. This Special Issue aims to highlight the discovery, characterization, and application of bioactive compounds derived from natural sources, with a focus on combating resistant strains and exploring innovative therapeutic strategies.

We warmly invite researchers to contribute original research articles and in-depth reviews. Areas of interest include, but are not limited to, the following:

  • Identification and isolation of novel antimicrobial compounds;
  • Mechanistic studies and biosynthetic pathways;
  • Synergistic effects with existing antibiotics;
  • Exploration of traditional medicinal plants and their derivatives.

This Special Issue offers a platform for presenting research that bridges the gap between natural product discovery and the pressing need for effective anti-infective solutions. Contributions that emphasize the transformative potential of natural ingredients in addressing the limitations of current antibiotic therapies are especially encouraged. We look forward to your valuable contributions.

Dr. Anca Zanfirescu
Guest Editor

Dr. Corina Andrei
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial resistance
  • antimicrobial activity
  • pathogens
  • infectious disorders
  • natural products
  • anti-infective agents

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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18 pages, 869 KiB  
Article
Comparison of In Vitro Methods for Assaying the Antibacterial Activity of a Mix of Natural Essential Oils Against Zoonotic Bacteria
by Karine Fayolle, Claire Girard, Pauline Lasfargues, Sahar Koteich and Sylvain Kerros
Microorganisms 2025, 13(5), 1125; https://doi.org/10.3390/microorganisms13051125 - 14 May 2025
Viewed by 211
Abstract
With the increasing occurrence of bacterial resistance, it is now essential to look for new alternatives to protect the curative utilization of antibiotics within the One Health concept. Here, we adapt and optimize a broth microdilution method and compare it against the broth [...] Read more.
With the increasing occurrence of bacterial resistance, it is now essential to look for new alternatives to protect the curative utilization of antibiotics within the One Health concept. Here, we adapt and optimize a broth microdilution method and compare it against the broth macrodilution method for evaluating the antibacterial activity of a complex essential oils mix (EO mix) against four livestock pathogens: Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus. Microdilution method performance (final volume well: 300 µL; inoculum: 1.0 × 106 CFU/mL) was evaluated following CLSI recommendations, by comparing the MIC of each of the four strains with the MICs obtained with the macrodilution method (final volume tube: 2 mL; inoculum 1.0 × 106 CFU/mL). Microdilution analysis was performed with an automated plate reader (Bioscreen C), and three bacterial growth parameters (OD max, lag phase, and growth rate) were calculated (DMFit curve-fitting software (v2.1; courtesy of the Institute of Food Research, Norwich, UK)). EO mix MICs were determined for E. coli, S. aureus, and B. cereus. Our results emphasize the importance of ensuring the accuracy of MIC results by performing three technical and three biological replicates, and combining OD max, lag phase, and growth rate to assess the impact of an EO mix at sub-MIC levels. Full article
(This article belongs to the Special Issue Advanced Research on Antimicrobial Activity of Natural Products)
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19 pages, 1580 KiB  
Article
Fungistatic and Bactericidal Activity of Hydroalcoholic Extracts of Root of Jatropha dioica Sessé
by Lizeth Aguilar-Galaviz, Jorge Cadena-Iñiguez, Dalia Abigail García-Flores, Gerardo Loera-Alvarado, Diego Rivera-Escareño and María Azucena Ortega-Amaro
Microorganisms 2025, 13(5), 1027; https://doi.org/10.3390/microorganisms13051027 - 29 Apr 2025
Viewed by 342
Abstract
Jatropha dioica Sessé (JD) is a plant from arid and semiarid zones of Mexico related to local therapeutic uses and possible use in food and agriculture as a control agent of pest organisms that helps to reduce impacts on the environment, human health [...] Read more.
Jatropha dioica Sessé (JD) is a plant from arid and semiarid zones of Mexico related to local therapeutic uses and possible use in food and agriculture as a control agent of pest organisms that helps to reduce impacts on the environment, human health and resistance by phytopathogens. In vitro bactericidal activity was evaluated with the well diffusion method in doses of 1000, 2500, 5000, 7500, 10,000 and 20,000 µg mL−1, and fungistatic activity was evaluated with the agar dilution method (500, 1000, 1500, 2000 and 4000 µg mL−1) in Pseudomonas syringae, Botrytis cinerea and Fusarium oxysporum using hydroalcoholic extracts of J. dioica root in a completely randomized design with five replications. Total phenol and flavonoid contents were recorded by the Folin–Ciocalteu and aluminum chloride methods. Ethanol and methanol extracts showed fungistatic activity on B. cinerea, inhibiting from 42.27 ± 1.09 to 46.68 ± 0.98 mg mL−1, with an IC50 of 5.04 mg mL−1, with no differences by solvent type. In F. oxysporum, inhibition ranged from 14.77 ± 1.08 to 29.19 ± 0.89 mg mL−1, and the methanol extract was more efficient, generating a stress response to the ethanol extract. The bactericidal activity on P. syringae recorded inhibition zones of 17.66 ± 0.33 and 16.66 ± 0.33 mg mL−1, with ethanol being more efficient. The phenol content ranged from 8.92 ± 0.25 to 12.10 ± 0.34 mg EAG g−1 and flavonoid content ranged from 20.49 ± 0.33 to 28.21 ± 0.73 mg QE g−1 of sample dry weight. The results highlight the biological activity of J. dioica as an alternative to biopesticides that minimize agrochemical applications and generate pathogen resistance. These advances contribute to the revaluation and conservation of the species. Full article
(This article belongs to the Special Issue Advanced Research on Antimicrobial Activity of Natural Products)
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21 pages, 654 KiB  
Article
Antibacterial Effects of Bulgarian Oregano and Thyme Essential Oils Alone and in Combination with Antibiotics Against Klebsiella pneumoniae and Pseudomonas aeruginosa
by Silviya Mihaylova, Antoaneta Tsvetkova, Sylvia Stamova, Neli Ermenlieva, Gabriela Tsankova, Emiliya Georgieva, Katya Peycheva, Veselina Panayotova and Yulian Voynikov
Microorganisms 2025, 13(4), 843; https://doi.org/10.3390/microorganisms13040843 - 7 Apr 2025
Viewed by 894
Abstract
Antimicrobial resistance (AMR) poses a global health threat, with multidrug-resistant (MDR) Klebsiella pneumoniae and Pseudomonas aeruginosa contributing to the burden, especially in Bulgaria. This study investigates recent resistance trends of these pathogens in Bulgaria and evaluates the antibacterial effects of commercially available oregano [...] Read more.
Antimicrobial resistance (AMR) poses a global health threat, with multidrug-resistant (MDR) Klebsiella pneumoniae and Pseudomonas aeruginosa contributing to the burden, especially in Bulgaria. This study investigates recent resistance trends of these pathogens in Bulgaria and evaluates the antibacterial effects of commercially available oregano (Origanum vulgare) and thyme (Thymus vulgaris) essential oils (EOs), both alone and in combination with conventional antibiotics. The chemical composition of the EOs was analyzed using gas chromatography–mass spectrometry (GC-MS), revealing carvacrol (84.2%) as the main component in oregano EO and thymol (45.74%) in thyme EO. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the EOs were determined using the serial dilution method. Oregano EO exhibited lower MIC and MBC values (0.039–1.250%) compared to thyme EO (0.156–5.000%) against both pathogens. The disk diffusion assay showed that oregano EO exhibits more potent antibacterial effects than thyme EO in Klebsiella pneumoniae and Pseudomonas aeruginosa strains. The findings highlight the potential of EOs as adjunctive therapies to enhance the efficacy of conventional antibiotics against MDR Klebsiella pneumoniae and Pseudomonas aeruginosa strains in Bulgaria. Full article
(This article belongs to the Special Issue Advanced Research on Antimicrobial Activity of Natural Products)
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