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Microorganisms
Special Issues
Recent Advances in Applied Microbiology 2.0
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Recent Advances in Applied Microbiology 2.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 3403

Special Issue Editor

Prof. Dr. Leticia M. Estevinho

E-Mail Website1 Website2
Guest Editor
Department of Biology and Biotechnology, School of Agriculture of the Polytechnic Institute of Bragança (ESA-IPB), Campus de Santa Apolónia, 5301-854 Bragança, Portugal
Interests: microbiology; food microbiology; food safety; healing; phenolic compounds; therapeutic properties; anti-inflammatory
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microbiology is an important branch of life sciences that has grown exponentially since the establishment of genomics, transcriptomics, and proteomics, which have made it possible to unravel the biogeochemical processes that microorganisms facilitate, as well as their interactions with macroorganisms in both health and disease. Microorganisms that were previously considered villains now play very important and beneficial roles in a wide variety of settings, including environment, food, agriculture, veterinary medicine, systematics, bioremediation, industry, medicine, and pharmaceuticals, leading to the emergence of the term “applied microbiology”. Additionally, microbiology has evolved through the discovery of new species, the selection and improvement of known strains, and the introduction of non-native genes for acquiring new expression products or functional traits. Market analysis corroborates the importance of this branch of life sciences; indeed, applied microbiology was valued at around USD 24.3 billion in 2017, and is expected to exceed USD 675.2 billion by 2024.

This Special Issue, entitled “Recent Advances in Applied Microbiology”, calls for reviews as well as original research articles documenting the progress and current understanding of different aspects of the vast field of applied microbiology.

Prof. Dr. Leticia M. Estevinho
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • applied microbiology
  • microorganisms
  • microbiology

Published Papers (2 papers)

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Research

18 pages, 793 KiB  
Article
Melipona scutellaris Geopropolis: Chemical Composition and Bioactivity
by Sónia Coutinho, Vanessa Matos, Natália Seixas, Hellen Rodrigues, Vanessa B. Paula, Lais Freitas, Teresa Dias, Francisco de Assis Ribeiro Santos, Luís G. Dias and Letícia M. Estevinho
Microorganisms 2023, 11(11), 2779; https://doi.org/10.3390/microorganisms11112779 - 15 Nov 2023
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Abstract
Geopropolis has been used in traditional medicine for centuries. In this study, the botanical origin, physicochemical profile, and biological activities of geopropolis from Melipona scutellaris harvested during rainy and dry seasons were investigated. Palynological analysis identified over 50 pollen types, with Schinus terebinthifolius [...] Read more.
Geopropolis has been used in traditional medicine for centuries. In this study, the botanical origin, physicochemical profile, and biological activities of geopropolis from Melipona scutellaris harvested during rainy and dry seasons were investigated. Palynological analysis identified over 50 pollen types, with Schinus terebinthifolius and Cecropia being the predominant types. The analytical results were in line with those reported in the literature. Rainy-season geopropolis exhibited higher total phenol and flavonoid content (determined using High Performance Liquid Chromatography—25.13% and 3.92%, respectively) compared to the dry season (19.30% and 2.09%); the major peaks (naringin, gallic acid, and catechin) were similar among samples. Antioxidant capacity was assessed via DPPH, reducing power, and β-carotene/linoleic acid discoloration assays. Rainy-season samples displayed superior antioxidant activity across methods. Antimicrobial effects were determined using microdilution, while the impact on the cholinesterase enzyme was quantified using 5-thio-2-nitrobenzoic acid accumulation. Anti-inflammatory and antimutagenic activities were assessed through hyaluronidase enzyme inhibition and by utilizing Saccharomyces cerevisiae ATCC-20113 cells. Both samples exhibited anti-inflammatory and antimutagenic properties. Moreover, a significant inhibition of acetylcholinesterase was observed, with IC50 values of 0.35 µg/mL during the rainy season and 0.28 µg/mL during the dry season. Additionally, the geopropolis displayed antimicrobial activity, particularly against Staphylococcus aureus. These findings suggest the therapeutic potential of M. scutellaris geopropolis in the context of inflammatory, oxidative, and infectious diseases. Full article
(This article belongs to the Special Issue Recent Advances in Applied Microbiology 2.0)
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14 pages, 702 KiB  
Article
Viable Mycobacterium avium subsp. paratuberculosis Colonizes Peripheral Blood of Inflammatory Bowel Disease Patients
by Maria Manuela Estevinho, José Cabeda, Mafalda Santiago, Elisabete Machado, Ricardo Silva, Mary Duro, Inês Pita, Rui Morais, Guilherme Macedo, Tim J. Bull, Fernando Magro and Amélia Sarmento
Microorganisms 2023, 11(6), 1520; https://doi.org/10.3390/microorganisms11061520 - 07 Jun 2023
Cited by 3 | Viewed by 2057
Abstract
Pathobionts, particularly Mycobacterium avium subsp. paratuberculosis (MAP) and Escherichia coli isolates with adherence/invasive ability (AIEC) have been associated with inflammatory bowel disease (IBD), particularly Crohn’s disease (CD). This study aimed to evaluate the frequency of viable MAP and AIEC in a cohort of [...] Read more.
Pathobionts, particularly Mycobacterium avium subsp. paratuberculosis (MAP) and Escherichia coli isolates with adherence/invasive ability (AIEC) have been associated with inflammatory bowel disease (IBD), particularly Crohn’s disease (CD). This study aimed to evaluate the frequency of viable MAP and AIEC in a cohort of IBD patients. As such, MAP and E. coli cultures were established from faecal and blood samples (with a total n = 62 for each) of patients with CD (n = 18), ulcerative colitis (UC, n = 15), or liver cirrhosis (n = 7), as well as from healthy controls (HC, n = 22). Presumptive positive cultures were tested by polymerase chain reaction (PCR), for a positive confirmation of MAP or E. coli identity. E. coli-confirmed isolates were then tested for AIEC identity using adherence and invasion assays in the epithelial cell line of Caco-2 and survival and replication assays in the macrophage cell line of J774. MAP sub-culture and genome sequencing were also performed. MAP was more frequently cultured from the blood and faecal samples of patients with CD and cirrhosis. E. coli presumptive colonies were isolated from the faecal samples of most individuals, in contrast to what was registered for the blood samples. Additionally, from the confirmed E. coli isolates, only three had an AIEC-like phenotype (i.e., one CD patient and two UC patients). This study confirmed the association between MAP and CD; however, it did not find a strong association between the presence of AIEC and CD. It may be hypothesized that the presence of viable MAP in the bloodstream of CD patients contributes to disease reactivation. Full article
(This article belongs to the Special Issue Recent Advances in Applied Microbiology 2.0)
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