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Special Issue Editor

Dr. Sharat Chandra Barman

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Guest Editor

Department of Chemistry, University of Florida, Gainesville, FL 32611, USA
Interests: electrochemical/optical immunosensors; CRISPR/Cas-based diagnostics; microfluidics; exosomal-miRNA-based detection; 2D nanomaterials; multiplexing assay

Special Issue Information

Dear Colleagues,

Nanomaterials as capture sites and enablers of signal transduction in biosensor applications have attracted a great deal of attraction from researchers in efforts to develop point-of-care sensors, disease model studies, and multiplexing assays. The ongoing COVID-19 pandemic requires multiplex detection systems with reliable, sensitive, reproducible, and selective features, and these technologies are also urgently needed for cancer diagnostics. Recently, many technologies, strategies, and nanomaterials are widely used for developing (bio)sensors.

Accordingly, this Special Issue invites contributions in the form of research papers and review articles that focus on nanomaterials-modified high-performance electrochemical and optical sensors, and on multiplexing assay development. Furthermore, with this Issue we aim to highlight the recent methods, limitations, and future directions for (bio)sensors and multiplexing assays.

We look forward to receiving your submissions!

Dr. Sharat Chandra Barman
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

multiplexing assays
nano/microscale (bio)sensors
exsosomal mrna detection
optical/electrochemical immunosensors
PCR-, RT-PCR-, and ELISA-based detection
Lab/organ-on-a-chip
2D nanomaterials, organic frameworks, and quantum dots for biosensing
sensors in agricultural science
wearable and flexible (bio)sensors for healthcare monitoring
single-molecule analysis
micro/nanofluidics

Published Papers (2 papers)

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Research

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12 pages, 1537 KiB

Open AccessArticle

Modular Micro Raman Reader Instrument for Fast SERS-Based Detection of Biomarkers

by Jamison Duckworth and Alexey V. Krasnoslobodtsev

Micromachines 2022, 13(10), 1570; https://doi.org/10.3390/mi13101570 - 22 Sep 2022

Cited by 1 | Viewed by 1472

Abstract

Sensitive detection of biomarkers is very critical in the diagnosis, management, and monitoring of diseases. Recent efforts have suggested that bioassays using surface-enhanced Raman scattering as a signal read-out strategy possess certain unique beneficial features in terms of sensitivity and low limits of detection which set this method apart from its counterparts such as fluorescence, phosphorescence, and radiolabeling. Surface-enhanced Raman scattering (SERS) has also emerged as an ideal choice for the development of multiplexed bioassays. Such promising features have prompted the need for the development of SERS-based tools suitable for point-of-care applications. These tools must be easy to use, portable, and automated for the screening of many samples in clinical settings if diagnostic applications are considered. The availability of such tools will result in faster and more reliable detection of disease biomarkers, improving the accessibility of point-of-care diagnostics. In this paper, we describe a modular Raman reader instrument designed to create such a portable device suitable for screening a large number of samples with minimal operator assistance. The device’s hardware is mostly built with commercially available components using our unique design. Dedicated software was created to automatically run sample screening and analyze the data measured. The mRR is an imaging system specifically created to automate measurements, eliminating human bias while enhancing the rate of data collection and analysis ~2000 times. This paper presents both the design and capabilities of the custom-built modular Raman reader system (mRR) capable of automated and fast measurements of sandwich immunoassay samples on gold substrates using modified gold nanoparticles as Raman tags. The limit of detection (LOD) of the tested MUC4-specific iSERS assay was measured to be 0.41 µg/mL. Full article

(This article belongs to the Special Issue Nanomaterials Modified Sensors and Multiplexing Assays)

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Review

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11 pages, 582 KiB

Open AccessReview

Electrical Impedance Tomography Technical Contributions for Detection and 3D Geometric Localization of Breast Tumors: A Systematic Review

by Juan Carlos Gómez-Cortés, José Javier Díaz-Carmona, José Alfredo Padilla-Medina, Alejandro Espinosa Calderon, Alejandro Israel Barranco Gutiérrez, Marcos Gutiérrez-López and Juan Prado-Olivarez

Micromachines 2022, 13(4), 496; https://doi.org/10.3390/mi13040496 - 23 Mar 2022

Cited by 8 | Viewed by 2705

Abstract

Impedance measuring acquisition systems focused on breast tumor detection, as well as image processing techniques for 3D imaging, are reviewed in this paper in order to define potential opportunity areas for future research. The description of reported works using electrical impedance tomography (EIT)-based techniques and methodologies for 3D bioimpedance imaging of breast tissues with tumors is presented. The review is based on searching and analyzing related works reported in the most important research databases and is structured according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) parameters and statements. Nineteen papers reporting breast tumor detection and location using EIT were systematically selected and analyzed in this review. Clinical trials in the experimental stage did not produce results in most of analyzed proposals (about 80%), wherein statistical criteria comparison was not possible, such as specificity, sensitivity and predictive values. A 3D representation of bioimpedance is a potential tool for medical applications in malignant breast tumors detection being capable to estimate an ap-proximate the tumor volume and geometric location, in contrast with a tumor area computing capacity, but not the tumor extension depth, in a 2D representation. Full article

(This article belongs to the Special Issue Nanomaterials Modified Sensors and Multiplexing Assays)

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