Research on Bacterial Cell Membranes

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Biological Membrane Functions".

Deadline for manuscript submissions: closed (31 December 2022) | Viewed by 2117

Special Issue Editors


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Guest Editor
S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco (UCDB), Campo Grande, MS, Brazil
Interests: peptide-based drug candidates; structure-guided design of antimicrobial peptides; peptidomimetics; molecular dynamics simulations of peptide-membrane, peptide-protein, protein-protein and membrane-receptor-peptide systems; nuclear magnetic resonance; bacterial resistance
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Special Issue Information

Dear Colleagues,

This Special Issue, entitled “Research on bacterial cell membranes”, focuses on studying bacterial membranes, bacterial extracellular vesicles (EVs), and mimetic membrane systems through biochemical, biophysical, and computational methods, including structural bioinformatics and omics strategies. Important aspects of this Special Issue include how the advances in bacterial membrane studies have assisted the design of selective antibacterial agents, the understanding of cell–cell interactions through EV secretion, and the modification of bacterial membrane components at the molecular level, including membrane proteins and lipids, for multidrug resistance events (spontaneous or induced).

This Special Issue aims to cover recent scientific contributions to the research on bacterial cell membranes, as well as review articles addressing the current advances and state of the art in this multidisciplinary field. Given your insightful works in the field, we invite you and your colleagues to contribute to this Special Issue, in which some leading experts will describe their works, ideas, and findings.

Dr. Marlon Henrique Cardoso
Dr. Sónia Gonçalves
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacterial membranes
  • bacterial extracellular vesicles
  • peptide–membrane interactions
  • peptide based-drugs
  • bacterial resistance

Published Papers (1 paper)

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Research

28 pages, 4823 KiB  
Article
Understanding Functional Redundancy and Promiscuity of Multidrug Transporters in E. coli under Lipophilic Cation Stress
by Mohammad S. Radi, Lachlan J. Munro, Jesus E. Salcedo-Sora, Se Hyeuk Kim, Adam M. Feist and Douglas B. Kell
Membranes 2022, 12(12), 1264; https://doi.org/10.3390/membranes12121264 - 14 Dec 2022
Cited by 2 | Viewed by 1602
Abstract
Multidrug transporters (MDTs) are major contributors to microbial drug resistance and are further utilized for improving host phenotypes in biotechnological applications. Therefore, the identification of these MDTs and the understanding of their mechanisms of action in vivo are of great importance. However, their [...] Read more.
Multidrug transporters (MDTs) are major contributors to microbial drug resistance and are further utilized for improving host phenotypes in biotechnological applications. Therefore, the identification of these MDTs and the understanding of their mechanisms of action in vivo are of great importance. However, their promiscuity and functional redundancy represent a major challenge towards their identification. Here, a multistep tolerance adaptive laboratory evolution (TALE) approach was leveraged to achieve this goal. Specifically, a wild-type E. coli K-12-MG1655 and its cognate knockout individual mutants ΔemrE, ΔtolC, and ΔacrB were evolved separately under increasing concentrations of two lipophilic cations, tetraphenylphosphonium (TPP+), and methyltriphenylphosphonium (MTPP+). The evolved strains showed a significant increase in MIC values of both cations and an apparent cross-cation resistance. Sequencing of all evolved mutants highlighted diverse mutational mechanisms that affect the activity of nine MDTs including acrB, mdtK, mdfA, acrE, emrD, tolC, acrA, mdtL, and mdtP. Besides regulatory mutations, several structural mutations were recognized in the proximal binding domain of acrB and the permeation pathways of both mdtK and mdfA. These details can aid in the rational design of MDT inhibitors to efficiently combat efflux-based drug resistance. Additionally, the TALE approach can be scaled to different microbes and molecules of medical and biotechnological relevance. Full article
(This article belongs to the Special Issue Research on Bacterial Cell Membranes)
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