New Trends in Image Analysis for Next-Generation Microscopy

Dear Colleagues,

Advancements in image analysis are pivotal in fully leveraging the potential of next-generation microscopy, enabling the extraction of meaningful insights from complex and high-resolution datasets. Our main objective for this Special Issue is to foster innovation in image analysis to enhance data interpretation, application efficiency, and access to advanced microscopy tools. The integration of emerging technologies, such as artificial intelligence, has revolutionized microscopy by enabling the automated and precise analysis of single-molecule, single-cell, and live-cell data. Moreover, advancements in image enhancement and super-resolution techniques have allowed researchers to explore biological, clinical, and material systems in unparalleled detail. The development of 3D and 4D image reconstruction and visualization techniques is expanding the horizons of spatial and temporal analysis, while multimodal data integration and quantitative approaches are providing holistic perspectives on complex biological and physical phenomena.

To meet the demands of large-scale and high-throughput applications, cloud computing and high-performance computing are increasingly essential in processing and analyzing microscopy datasets. Additionally, the advent of open hardware and software solutions is democratizing access to advanced microscopy technologies, fostering innovation and collaboration across disciplines. Applications go far beyond clinical research, encompassing biological studies on cellular dynamics, materials science investigations into nanostructures, and environmental analyses of microbial ecosystems and pollutants.

This Special Issue will feature a selection of contributions presented at the recent SPAOM 2024 Conference. By showcasing the latest advancements in image analysis for next-generation microscopy, we aim to inspire innovative solutions and promote collaborations to facilitate discoveries across the diverse applications of microscopy. Papers are solicited from both the Conference and the broader community. We are looking for papers including, but not limited to, the following areas:

• Machine learning and deep learning in microscopy;
• Image analysis, image enhancement, and super-resolution techniques;
• Single-molecule, single-cell, and live-cell image analysis;
• Nonlinear imaging and advanced microscopy (SHG, THG, CARS, FlIM, or FRET);
• 3D and 4D image reconstruction and visualization;
• Automated quantitative analysis and high-content screening;
• Multimodal integration and correlative imaging;
• Cloud and high-performance computing;
• Open hardware and software in microscopy.

Dr. María Viñas Peña
Dr. Gloria Bueno
Dr. Gabriel Cristobal
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Imaging is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• microscopy
• image analysis
• correlative imaging
• deep learning
• nanoscopy
• super-resolution techniques
• live cell analysis
• 3D and 4D visualization
• cloud computing
• open hardware and software