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Topical Collection "Hemiptera: Ecology, Physiology, and Economic Importance"

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A topical collection in Insects (ISSN 2075-4450). This collection belongs to the section "Insect Physiology, Reproduction and Development".

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Editors

Dr. Sergey Ya. Reznik

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Collection Editor

Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia
Interests: insects; ecology; physiology; diapause; photoperiod; temperature

Dr. Dmitrii L. Musolin

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Collection Editor

European and Mediterranean Plant Protection Organization (EPPO/OEPP), Paris, France
Interests: Heteroptera seasonal development; diapause; responses to climate change

Dr. Anne L. Nielsen
E-Mail Website
Collection Editor

Rutgers Agricultural Research and Extension Center, Rutgers University, Bridgeton, NJ 08302, USA
Interests: physiology and behavior of insects; phenological models for pests of tree fruits and small fruits

Topical Collection Information

Dear Colleagues,

Hemiptera is one of the largest insect orders playing an important role in both natural and anthropogenic ecosystems. Numerous phytophagous hemipterans cause serious damage to agriculture and forestry, whereas some predatory species are widely used as biocontrol agents. Therefore, investigations conducted on Hemiptera are of interest for both fundamental and applied entomology. This Topical Collection aims to represent the current state of the studies on Hemiptera ecology and physiology. Both original research articles and reviews can be submitted; studies on ecophysiology (regulation of seasonal cycles, reproduction, feeding, locomotion, etc.) are particularly welcome; preference will be given to the papers concerning economically important species (both pests and beneficial ones). We look forward to receiving your valuable contributions.

Dr. Sergey Ya. Reznik
Dr. Dmitrii L. Musolin
Dr. Anne L. Nielsen
Collection Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Insects is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

Hemiptera
ecology
physiology
insect pests
plant protection
biological control

Published Papers (16 papers)

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2023

Jump to: 2022, 2021

Open AccessArticle

The Invasive Caucasian Populations of the Brown Marmorated Stink Bug Halyomorpha halys (Hemiptera: Heteroptera: Pentatomidae) Rapidly Adapt Their Ecophysiological Traits to the Local Environmental Conditions

Sergey Ya. Reznik

Margarita Yu. Dolgovskaya

Natalia N. Karpun

Vilena Ye. Zakharchenko

Aida Kh. Saulich

and

Dmitrii L. Musolin

Insects 2023, 14(5), 424; https://doi.org/10.3390/insects14050424 - 29 Apr 2023

Viewed by 916

Abstract

The ability to rapidly adapt to new environmental conditions is a crucial prerequisite for the wide-scale invasion of pests or intentional introduction of beneficial insects. A photoperiodically induced facultative winter diapause is an important adaptation ensuring synchronization of insect development and reproduction with the local seasonal dynamics of environmental factors. We conducted a laboratory study aimed to compare photoperiodic responses of two invasive Caucasian populations of the brown marmorated stink bug Halyomorpha halys (Hemiptera: Heteroptera: Pentatomidae), which recently invaded neighboring regions with subtropical (Sukhum, Abkhazia) and temperate (Abinsk, Russia) climates. Under the temperature of 25 °C and the near-critical photoperiods of L:D = 15:9 h and 15.5:8.5 h, the population from Abinsk showed a slower pre-adult development and a stronger tendency to enter winter adult (reproductive) diapause compared to the population from Sukhum. This finding agreed with the difference between the local dynamics of the autumnal temperature decrease. Similar adaptive interpopulation differences in the patterns of diapause-inducing responses are known in other insect species but our finding is distinguished by a very short adaptation time: H. halys was first recorded in Sukhum in 2015 and in Abinsk in 2018. Thus, the differences between the compared populations might have evolved over a relatively short span of several years. Full article

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Open AccessArticle

The Male Reproductive System of the Kissing Bug, Rhodnius prolixus Stål, 1859 (Hemiptera: Reduviidae: Triatominae): Arrangements of the Muscles and the Myoactivity of the Selected Neuropeptides

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Insects 2023, 14(4), 324; https://doi.org/10.3390/insects14040324 - 28 Mar 2023

Viewed by 1403

Abstract

The gross anatomy of the male reproductive structures and their associated musculature are described in the blood-gorging vector of Chagas disease, Rhodnius prolixus. The male reproductive system is composed of muscular tissues each performing contractions that aid in the movement of sperm out of the testis into the vas deferens, seminal vesicle and then into the ejaculatory duct, along with proteins and lipids from the transparent and opaque accessory glands. Phalloidin staining shows the various patterns of muscle fiber layers, from thin circular to more complex crisscross patterns, implying subtle differences in the form of the contractions and movement of each of the structures, allowing for waves of contractions or twisting patterns. The transcripts for the receptors for proctolin, myosuppressin (RhoprMS) and for the extended FMRFamides are expressed in the various regions of the reproductive system, and the nerve processes extending over the reproductive structures are positive for FMRFamide-like immunoreactivity, as are neurosecretory cells lying on the nerves. Proctolin and AKDNFIRFamide are strong stimulators for the frequency of the contractions, and RhoprMS can inhibit the proctolin-induced contractions. Taken together, this work implicates these two families of peptides in coordinating the male reproductive structures for the successful transfer of sperm and the associated accessory gland fluid to the female during copulation. Full article

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Open AccessArticle

Temperature-Dependent Life Table Parameters of Brown Marmorated Stink Bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) in the United States

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Insects 2023, 14(3), 248; https://doi.org/10.3390/insects14030248 - 02 Mar 2023

Cited by 1 | Viewed by 1137

Abstract

Brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), is a generalist pest that causes serious injury to a variety of crops around the world. After the first detection in the USA, H. halys became a serious threat to growers resulting in significant crop damage. Understanding the effect of temperature on H. halys development will help to achieve successful control by predicting the phenological timing of the pest. Here, life table parameters (survival, development, reproduction, and daily mortality) of H. halys were evaluated for New Jersey and Oregon populations in the US. Parameters were determined from field-collected and laboratory-reared individuals. The results indicated that New Jersey populations had higher levels of egg-laying than Oregon populations and exhibited higher and earlier fecundity peaks. Survival levels were similar between populations. Linear and nonlinear fit were used to estimate the minimum (14.3 °C), optimal (27.8 °C), and maximum (35.9 °C) temperatures where development of H. halys can take place. An age-specific fecundity peak (M_x = 36.63) was recorded at 936 degree days for New Jersey populations, while maximum fecundity (M_x = 11.85) occurred at 1145 degree days in Oregon. No oviposition was recorded at the lowest (15 °C) or highest (35 °C) trialed temperatures. Developmental periods increased at temperatures above 30 °C, indicating that such higher temperatures are suboptimal for H. halys development. Altogether the most optimal temperatures for population increase (r_m) ranged from 25 to 30 °C. Survival rates of H. halys at suboptimal low temperatures of 8 °C (i.e., 61%) is comparable to previous reports. The present paper provides additional data and context from a range of experimental conditions and populations. Such temperature-related H. halys life table parameters can be used to provide determine the risk to susceptible crops. Full article

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2022

Jump to: 2023, 2021

Open AccessArticle

Comparative Studies of Perianal Structures in Myrmecophilous Aphids (Hemiptera, Aphididae)

Natalia Kaszyca-Taszakowska

Mariusz Kanturski

and

Łukasz Depa

Insects 2022, 13(12), 1160; https://doi.org/10.3390/insects13121160 - 16 Dec 2022

Cited by 2 | Viewed by 1180

Abstract

There are three types of relationships between aphids and ants: non-myrmecophilous, obligatory and facultatively myrmecophilous. The degree of involvement in this mutualism is believed to be corelated with morphological adaptations of perianal structures. In this manuscript, we analyzed the differences of these structures in obligatorily (18 species) and facultatively (10 species) myrmecophilous aphids. Scanning electron microscopy (SEM) and light stereoscopic microscopy (LSM) techniques were used for these studies. Comparison of structures showed no strict relationship between their morphology and the degree myrmecophily, with certain indication that the microsculpture of perianal cuticle may play role in protection of aphids against honeydew droplet in facultatively myrmecophilous aphids. Full article

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Open AccessArticle

Inheritance and Biological Characterization of an Orange-nymph Mutant in Orius laevigatus (Hemiptera: Anthocoridae)

Amador Rodríguez-Gómez

Alberto Donate

Isabel Sánchez-Martínez

Virginia Balanza

Ana Belén Abelaira

María del Carmen Reche

and

Pablo Bielza

Insects 2022, 13(11), 996; https://doi.org/10.3390/insects13110996 - 30 Oct 2022

Viewed by 2092

Abstract

A mutation showing a distinct orange color in the nymph stages was found in Orius laevigatus (Fieber) (Hemiptera: Anthocoridae), a key biological control agent in protected crops, used to control small pests, especially thrips. A laboratory strain carrying this body color mutation ambar was established. Genetic analysis determined that the mutation (ambar) was controlled by a single autosomal recessive allele. Some biological and ecological characteristics of this orange strain were compared to a normal population. Longevity, fecundity and fertility were similar in both populations, but immature survival, development rate, body size, starvation tolerance and predation capacity were inferior in the orange strain. The utility of the orange mutant as a visible marker for biological and ecological studies of this important biological control agent is discussed. Full article

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Open AccessArticle

RNAi-Mediated Manipulation of Cuticle Coloration Genes in Lygus hesperus Knight (Hemiptera: Miridae)

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Insects 2022, 13(11), 986; https://doi.org/10.3390/insects13110986 - 27 Oct 2022

Cited by 1 | Viewed by 1289

Abstract

Cuticle coloration in insects is a consequence of the accumulation of pigments in a species-specific pattern. Numerous genes are involved in regulating the underlying processes of melanization and sclerotization, and their manipulation can be used to create externally visible markers of successful gene editing. To clarify the roles for many of these genes and examine their suitability as phenotypic markers in Lygus hesperus Knight (western tarnished plant bug), transcriptomic data were screened for sequences exhibiting homology with the Drosophila melanogaster proteins. Complete open reading frames encoding putative homologs for six genes (aaNAT, black, ebony, pale, tan, and yellow) were identified, with two variants for black. Sequence and phylogenetic analyses supported preliminary annotations as cuticle pigmentation genes. In accord with observable difference in color patterning, expression varied for each gene by developmental stage, adult age, body part, and sex. Knockdown by injection of dsRNA for each gene produced varied effects in adults, ranging from the non-detectable (black 1, yellow), to moderate decreases (pale, tan) and increases (black 2, ebony) in darkness, to extreme melanization (aaNAT). Based solely on its expression profile and highly visible phenotype, aaNAT appears to be the best marker for tracking transgenic L. hesperus. Full article

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Open AccessArticle

Biological Parameters, Phenology and Temperature Requirements of Halyomorpha halys (Hemiptera: Pentatomidae) in the Sub-Mediterranean Climate of Western Slovenia

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Insects 2022, 13(10), 956; https://doi.org/10.3390/insects13100956 - 19 Oct 2022

Cited by 2 | Viewed by 1373

Abstract

In the last decade, the invasive brown marmorated stink bug Halyomorpha halys, native to East Asia, has become one of the most serious pests for agricultural crops worldwide. First detected in Europe (in Switzerland), the insect is now widely found across the European continent and many Eurasian countries. Since its first appearance in Slovenia in 2017 it has caused considerable damage to fruit and vegetable production. Understanding the biology and behavior in the local environmental conditions is of key importance for an effective pest management. Knowledge of the voltinism of the species is crucial to anticipate critical phases of pest development and for adapting control measures that target the vulnerable life stages of the pest. A 3-year study (2019–2021) of H. halys biological parameters was performed outdoors in Nova Gorica (western Slovenia), confirming that in the sub-Mediterranean climate this pest has two overlapping generations per year. The net reproductive rates observed in the studied period indicate growing populations. The highest population growth was recorded in 2019, when the net reproductive rate of increase (R₀) reached 14.84 for the summer generation and 5.64 for the overwintering generation. These findings match the current situation in Slovenia, where increasing populations of H. halys and severe crop damage have been observed since 2019. Full article

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Open AccessReview

Natural Enemies and Biological Control of Stink Bugs (Hemiptera: Heteroptera) in North America

Blessing Ademokoya

Kacie Athey

and

John Ruberson

Insects 2022, 13(10), 932; https://doi.org/10.3390/insects13100932 - 14 Oct 2022

Viewed by 2185

Abstract

Stink bugs comprise a significant and costly pest complex for numerous crops in the US, including row crops, vegetables, and tree fruits and nuts. Most management relies on the use of broad-spectrum and disruptive insecticides with high human and environmental risks associated with them. Growing concerns about pesticide resistance in stink bugs are forcing pest managers to explore safer and more sustainable options. Here, we review the diverse suite of natural enemies of stink bugs in the US, noting that the egg and the late nymphal and adult stages of stink bugs are the most commonly attacked by parasitoids, whereas eggs and young nymphs are the stages most commonly attacked by predators. The effectiveness of stink bugs’ natural enemies varies widely with stink bug species and habitats, influencing the biological control of stink bugs across crops. Historically, biological control of stink bugs has focused on introduction of exotic natural enemies against exotic stink bugs. Conservation and augmentation methods of biological control have received less attention in the US, although there may be good opportunities to utilize these approaches. We identify some considerations for the current and future use of biological control for stink bugs, including the potential for area-wide management approaches. Full article

Open AccessArticle

Effect of Male Body Size on Female Reproduction in Pyrrhocoris apterus (L.) (Heteroptera, Pyrrhocoridae)

Alois Honek

and

Zdenka Martinkova

Insects 2022, 13(10), 902; https://doi.org/10.3390/insects13100902 - 03 Oct 2022

Viewed by 967

Abstract

Females and males of the abundant heteropteran species Pyrrhocoris apterus (L.) mate with the larger of the available partners. The male benefits from copulation with a large female, which is more productive than a small female. However, the benefit to females from copulation with a large male has not yet been investigated. Overwintered sexually immature adults were transferred from the field to indoors for a long day at 25 °C and subsequently kept as female-male pairs with different partner size ratios. The female lays eggs in several successive clutches. We investigated the influence of male size on the number and weight of eggs in individual clutches and the time elapsed from the transfer to their deposition. These characteristics of reproduction were first regressed on female size, and residuals of this regression were then regressed on male size. The positive effect of increasing male size on the characteristics of female reproduction manifested as a significant value of the latter regressions. The expected positive effect of increasing male body size on female reproductive characteristics was not detected. Several reasons for this deficiency are discussed. Full article

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Open AccessArticle

Females of Halyomorpha halys (Hemiptera: Pentatomidae) Experience a Facultative Reproductive Diapause in Northern Greece

Eleni I. Koutsogeorgiou

Nikos A. Kouloussis

Dimitrios S. Koveos

and

Stefanos S. Andreadis

Insects 2022, 13(10), 866; https://doi.org/10.3390/insects13100866 - 23 Sep 2022

Cited by 3 | Viewed by 1063

Abstract

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is a native pest of East Asia that overwinters as an adult in natural and human-made structures. Adult emergence from overwintering sites starts in spring, whereas females produce offspring in early summer on host plants, where most feeding occurs. In this study, we investigated the reproductive physiology of overwintering females of H. halys in Northern Greece, by determining the duration of the preoviposition period and fecundity of individuals that were left to overwinter in natural conditions and were subsequently transferred to chambers with standard conditions monthly, from December 2020 to March 2021. According to our results, overwintering H. halys females do not initiate egg laying once they emerge from overwintering sites, but rather need some additional time to exit diapause and mature reproductively. The mean preoviposition period of overwintering females that were transferred from their overwintering sites to the chambers in December 2020 was 29.0 days, which was significantly longer by 8.3 days than that of females that overwintered until March 2021, and by 13.2 days than the control (26 °C, 60% RH and a 16:8 h light: dark photoperiod). No significant difference among the average number of eggs per egg mass laid by overwintering individuals brought in the chambers in different time intervals and the laboratory colony was observed. However, females that were left to overwinter until March laid a significantly higher number of eggs in total, compared to the ones whose overwintering was disrupted in February. Based on our findings, overwintering females of H. halys experience a facultative reproductive diapause in Northern Greece. Our study was the first to determine the occurrence of diapause of H. halys in N. Greece and our findings could be very valuable for assessing the damage of this pest to early-season crops and designing successful management practices. Full article

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Open AccessArticle

Effect of Parental Age and Mating Status on Reproductive Performance of Orius laevigatus (Hemiptera: Anthocoridae)

Amador Rodríguez-Gómez

Virginia Balanza

Alberto Donate

Ana Belén Abelaira

María del Carmen Reche

Isabel Sánchez-Martínez

and

Pablo Bielza

Insects 2022, 13(9), 827; https://doi.org/10.3390/insects13090827 - 12 Sep 2022

Cited by 1 | Viewed by 968

Abstract

The reproductive potential of biological control agents (BCAs) is crucial for efficient mass-rearing and field performance, and it all begins with mating. Fecundity can be strongly influenced by intrinsic conditions, such as female age and, often neglected, male age and mating status. However, little is known about the impact of parental status at mating on female reproductive outcomes in BCAs. Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) is widely used to control thrips in protected crops. We evaluated how many consecutive females a male could successfully mate and the effect on a female’s reproductive output. In addition, we studied the effects of male and female age on mating. In the multiple mating experiment, the males showed a high capacity to fertilize females successively, not reducing fecundity until the sixth mated female. In the delayed mating experiment, copulation duration and fecundity increased with male age but decreased with female age. In contrast, fertility followed an opposite pattern, increasing with female age but decreasing with male age. However, fecundity gains outweighed fertility declines in both sexes. Therefore, reproductive capacity is increased when mating newly emerged females with males a few days old. The implications of our results for mass rearing and field performance are discussed. Full article

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Open AccessArticle

Evaluation of Spatial Distribution of Three Major Leptocorisa (Hemiptera: Alydidae) Pests Using MaxEnt Model

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Insects 2022, 13(8), 750; https://doi.org/10.3390/insects13080750 - 20 Aug 2022

Cited by 1 | Viewed by 1689

Abstract

We targeted three major Leptocorisa species (L. chinensis, L. acuta, and L. oratoria) and evaluated their potential distributions using MaxEnt. The results showed that most Asian countries and northern Australia would be suitable for at least one of these pest species, and climate change will expand their habitat northward. All of the developed models were evaluated to be excellent with AUC, TSS, and OR10%. Most of the recorded regions of the Leptocorisa species are consistent with the result of potential distributions predicted in this study. The results confirmed that the minimum temperature of the coldest month mainly influences the three Leptocorisa species distributions. The potential distributions of the three species cover major rice cultivation areas regardless of climate change, suggesting that it would be necessary to establish a sustainable control strategy for the pests. Full article

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Open AccessArticle

New Records and Updated Checklist of the Pentatomoidea (Hemiptera: Heteroptera) of Greece

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Insects 2022, 13(8), 749; https://doi.org/10.3390/insects13080749 - 19 Aug 2022

Cited by 1 | Viewed by 1125

Abstract

Eight species of the superfamily Pentatomoidea are recorded from Greece for the first time: Aelia germari Küster 1852, Eurygaster hottentotta (Fabricius 1775), Eysarcoris aeneus (Scopoli 1763), Neottiglossa lineolata (Herrich-Schaeffer 1830), Neottiglossa pusilla (Gallen 1789), Picromerus bidens (Linnaeus 1758), Podops (Podops) inunctus (Fabricius 1775) [...] Read more.

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Open AccessArticle

To Every Thing There Is a Season: Phenology and Photoperiodic Control of Seasonal Development in the Invasive Caucasian Population of the Brown Marmorated Stink Bug, Halyomorpha halys (Hemiptera: Heteroptera: Pentatomidae)

Sergey Ya. Reznik

Natalia N. Karpun

Vilena Ye. Zakharchenko

Yelena I. Shoshina

Margarita Yu. Dolgovskaya

Aida Kh. Saulich

and

Dmitry L. Musolin

Insects 2022, 13(7), 580; https://doi.org/10.3390/insects13070580 - 25 Jun 2022

Cited by 6 | Viewed by 1490

Abstract

Studies on the phenology of local populations of invasive insects are necessary for monitoring and predicting their dispersion. We investigated the phenology of the brown marmorated stink bug, Halyomorpha halys, in the Sochi region (Krasnodar Territory, Russia) from 2018 to 2021 by regular field sampling and dissecting. The results of the sampling suggest that H. halys is at least partially bivoltine in the studied region: the main period of mass oviposition (by the overwintered females) occurs from June to July; the second, much shorter period of egg-laying (by females of the new, i.e., the first generation) occurs in August. Reproductively active individuals (i.e., females with developed ovaries and filled spermatheca and males with filled ectodermal sac) were recorded from the end of May to the beginning of September. Such a seasonal pattern correlated with day length: when the natural photoperiod decreased below the experimentally determined critical day length (15.0–15.5 h), the proportions of females with fully developed ovaries sharply dropped to zero. Both the rate of H. halys pre-adult development and the timing of the induction of winter adult diapause observed under natural conditions fully agreed with the earlier predictions that had been based on the results of laboratory experiments. Full article

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Open AccessArticle

Flying over Eurasia: Geographic Variation of Photoperiodic Control of Nymphal Development and Adult Diapause Induction in Native and Invasive Populations of the Brown Marmorated Stink Bug, Halyomorpha halys (Hemiptera: Heteroptera: Pentatomidae)

Dmitry L. Musolin

Margarita Yu. Dolgovskaya

Vilena Ye. Zakharchenko

and

Insects 2022, 13(6), 522; https://doi.org/10.3390/insects13060522 - 04 Jun 2022

Cited by 4 | Viewed by 1680

Abstract

Facultative winter adult diapause in Halyomorpha halys is regulated by a long-day photoperiodic response. Day length also influences nymphal development, which slows down at the critical (near-threshold) day lengths. We compared the photoperiodic responses of one native (Andong, South Korea) and three invasive (Torino, Italy; Basel, Switzerland; and Sochi, Russia) populations in a laboratory common-garden experiment. Nymphs developed and emerging adults were reared at 24 °C in a range of photoperiods with day lengths of 14.0, 14.5, 15.0, 15.5, and 16.0 h. The critical day lengths of the photoperiodic responses of both sexes fell between 14.5 and 15.0 h in the native Korean population and between 15.0 and 15.5 h in three invasive European populations. The differences between the three invasive populations were not significant, despite their distant origins. Moreover, the difference between the Korean and European populations was much smaller than was expected. The microevolution was possibly ‘too slow to keep up’ with the rapid spread of the invader across Eurasia. It is expected that soon the critical day length of the invasive H. halys populations will gradually change to adapt better to local conditions. At present, the critical day length for diapause induction of 15 h 15 min can be used to model the phenology, further spread, and response to climate change for all European populations of the pest. Full article

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2021

Jump to: 2023, 2022

Open AccessArticle

Identification and Characterization Analysis of Transient Receptor Potential Mucolipin Protein of Laodelphax striatellus Fallén

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Insects 2021, 12(12), 1107; https://doi.org/10.3390/insects12121107 - 12 Dec 2021

Cited by 1 | Viewed by 1829

Abstract

Transient receptor potential mucolipin (TRPML) protein in flies plays a pivotal role in Ca²⁺ ions release, resulting in membrane trafficking, autophagy and ion homeostasis. However, to date, the characterization of TRPML in agricultural pests remains unknown. Here, we firstly reported the TRPML of a destructive pest of gramineous crops, Laodelphax striatellus. The L. striatellus TRPML (Ls-TRPML) has a 1818 bp open reading frame, encoding 605 amino acid. TRPML in agricultural pests is evolutionarily conserved, and the expression of Ls-TRPML is predominately higher in the ovary than in other organs of L. striatellus at the transcript and protein level. The Bac–Bac system showed that Ls-TRPML localized in the plasma membrane, nuclear membrane and nucleus and co-localized with lysosome in Spodoptera frugiperda cells. The immunofluorescence microscopy analysis showed that Ls-TRPML localized in the cytoplasm and around the nuclei of the intestine cells or ovary follicular cells of L. striatellus. The results from the lipid-binding assay revealed that Ls-TRPML strongly bound to phosphatidylinositol-3,5-bisphosphate, as compared with other phosphoinositides. Overall, our results helped is identify and characterize the TRPML protein of L. striatellus, shedding light on the function of TRPML in multiple cellular processes in agricultural pests. Full article

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Published Papers (16 papers)

2023

Jump to: 2022, 2021

2022

Jump to: 2023, 2021

2021

Jump to: 2023, 2022

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