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Bioactive Phenolics and Polyphenols 2025

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Bioactives and Nutraceuticals".

Deadline for manuscript submissions: 20 March 2026 | Viewed by 416

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Special Issue Information

Dear Colleagues,

The scientific interest in plant phenolics as chemopreventive and therapeutic agents against chronic and degenerative diseases has been increasing since the late 1990s, when the French paradox was associated with the high intake of polyphenols present in red wine. Since then, research advances include their biosynthesis, biological activities, purification, and chemical characterization in different plant species. Furthermore, research on the stability of phenolics in food processing techniques and storage is an area of major interest.

Contributions to this Special Issue may cover all aspects of phenolics and polyphenols including their chemical characterization on different plant species and methods for their extraction, purification, and quantification; elucidation of their mechanism of action; development of innovative methods for evaluating their bioactivity in vitro and in vivo; evaluation of their stability to thermal and non-thermal food processing techniques; elicitation of the plant phenolic biosynthesis pathway; and the effects of postharvest handling on the phenolic profiles of plant foods. Papers on developing food products and dietary supplements enriched with plant phenolics will be also be considered.

Prof. Dr. Davide Barreca
Guest Editor

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Keywords

  • phenolic metabolism
  • biological activities
  • stability to food processing
  • elicitation
  • methods of analysis
  • dietary supplements
  • nutraceutical ingredients
  • non-thermal processing
  • extraction and purification

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Published Papers (1 paper)

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Research

17 pages, 1383 KB  
Article
Determination of Gnetol in Murine Biological Matrices by Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS): Application in a Biodistribution Study
by Boyu Liao, Hongrui Jin, Huan Chen, Yuxin Zhang, Xuexian Deng, Jingyi Yao, Na Li, Shaoshu Xu, Jingbo Wang, Mingming Gao, Xiaoying Zhang, Paul C. L. Ho, Hui Liu and Hai-Shu Lin
Int. J. Mol. Sci. 2025, 26(21), 10358; https://doi.org/10.3390/ijms262110358 - 24 Oct 2025
Viewed by 153
Abstract
Gnetol (trans-2,3′,5′,6-tetrahydroxystilbene), a naturally occurring stilbene structurally related to resveratrol (trans-3,5,4′-trihydroxystilbene; RES), has been reported to possess multiple health-promoting activities. In order to support its potential nutraceutical application, a reliable chromatography–tandem mass spectrometry (LC–MS/MS) assay was developed and validated [...] Read more.
Gnetol (trans-2,3′,5′,6-tetrahydroxystilbene), a naturally occurring stilbene structurally related to resveratrol (trans-3,5,4′-trihydroxystilbene; RES), has been reported to possess multiple health-promoting activities. In order to support its potential nutraceutical application, a reliable chromatography–tandem mass spectrometry (LC–MS/MS) assay was developed and validated for the quantitative determination of gnetol in mouse plasma and tissue samples, using isotopically labeled RES-13C6 serving as the internal standard (IS). Electrospray ionization (ESI) was performed in negative mode, with multiple reaction monitoring (MRM) transitions m/z 243.2 → 175.0 for gnetol and m/z 233.1 → 191.0 for the IS. Chromatographic separation was achieved on a reversed-phase HPLC column using a 5-min gradient delivery of acetonitrile and 2 mM ammonium acetate at 0.5 mL/min and 40 °C. The linear calibration curve covered the concentration range of 5.0–1500 ng/mL, and the method validation confirmed its selectivity, accuracy, precision, stability, and dilution integrity. The developed method was subsequently applied to a biodistribution study in mice after oral administration of gnetol at 400 µmol/kg (equivalent to 97.7 mg/kg). Gnetol was rapidly absorbed and extensively distributed in key pharmacologically relevant organs. Despite its poor aqueous solubility, oral uptake was not significantly hindered. Collectively, these findings demonstrate that gnetol exhibits favorable absorption and tissue distribution profiles, supporting its promise as a candidate for nutraceutical development. Full article
(This article belongs to the Special Issue Bioactive Phenolics and Polyphenols 2025)
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