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Whole-Cell System and Synthetic Biology, 2nd Edition

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Guest Editor
Key Laboratory of Food Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Interests: synthesis biology; molecular biology; functional nucleic acids; biosensor
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Special Issue Information

Dear Colleagues,

The whole-cell system has been of great interest recently and has advanced the analysis and manufacturing techniques in the past decade, benefitting from the development of molecular biology and synthetic biology. The understanding of cell metabolism and gene regulation at the molecular level is of particular significance. The understanding of how the whole-cell system is applied in analysis or manufacturing has progressed, but there is still a great deal to be learnt. Key issues gaps in the area are as follows: (1) Gene interactions and regulatory networks still need to be explored. (2) Technical approaches need to be upgraded to edit the whole cell system. (3) The performance of the whole-cell system needs to be improved. (4) How to effectively apply the whole cell system in food, the environment and other fields needs to be understood. Therefore, the aim of this Special Issue is to summarize and broaden the knowledge of new gene circuits, innovative molecular modification methods and the application of whole cell systems.

Authors are invited to submit original research and review articles that address the areas discussed above. Topics include but are not limited to the following:

  • Identification and mining of new molecular networks in whole-cell systems;
  • Methods for upgrading and the modification of whole-cell systems;
  • Application of whole-cell systems in food, environment and other fields.

Dr. Jingjing Tian
Guest Editor

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Keywords

  • synthesis biology
  • molecular biology
  • functional nucleic acids
  • biosensor
  • CRISPR-Cas
  • directed evolution
  • whole-cell system

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Published Papers (1 paper)

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Research

16 pages, 1717 KiB  
Article
Utilization of Crab Shell Waste for Value-Added Bioplastics by Pseudomonas-Based Microbial Cell Factories
by Xiaofen Song, Hansheng Wei, Yueyue Zhou, Weiwei Song, Ce Shi, Changkao Mu, Chunlin Wang and Xiaopeng Wang
Int. J. Mol. Sci. 2025, 26(6), 2543; https://doi.org/10.3390/ijms26062543 - 12 Mar 2025
Viewed by 564
Abstract
With the development of the aquatic products processing industry, 6–8 million tons of shrimp and crab shell waste are produced globally annually, but, due to the lack of high-value conversion technology, crab shells are often discarded in large quantities as a by-product of [...] Read more.
With the development of the aquatic products processing industry, 6–8 million tons of shrimp and crab shell waste are produced globally annually, but, due to the lack of high-value conversion technology, crab shells are often discarded in large quantities as a by-product of processing. Pseudomonas-based microbial cell factories are capable of biosynthesis of high-value products using a wide range of substrates; however, there is currently no reliable fermentation model for producing high-value chemicals using crab shell waste by Pseudomonas strains. In this study, we first explored the culture conditions of shell fermentation using KT2440 through single-factor and orthogonal experiments, and the optimized fermentation parameters obtained are given as follows: a temperature of 30 °C, fermentation time of 42 h, substrate solid–liquid ratio of 7%, and rotational speed of 200 rpm. After optimization, the maximum cell growth was increased by 64.39% from 350.67 × 108 CFU/mL to 576.44 × 108 CFU/mL. Combined with engineering modification, two engineered strains, KT+IV and KT+lasBT, expressing exogenous proteases, were obtained, and the maximum growth was increased from 316.44 × 108 CFU/mL to 1268.44 × 108 CFU/mL and 616.89 × 108 CFU/mL, which were 300.84% and 94.94% higher, respectively. In addition, the engineered strain KT+NtrcT-D55E, which regulates nitrogen metabolism, was obtained, and the accumulation of intracellular polyhydroxy fatty acid esters (PHA) was increased from 20.00 mg/L to 78.58 mg/L, which was a significant increase of 292.93% relative to the control group. This study provides a theoretical basis and technical support for the high-value utilization of shrimp and crab shell resources and the development of environmentally friendly bioproducts. Full article
(This article belongs to the Special Issue Whole-Cell System and Synthetic Biology, 2nd Edition)
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