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The Application of Biomaterials in Bone Tissue Repair and Regeneration, 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: 30 May 2026 | Viewed by 726

Special Issue Editor

Special Issue Information

Dear Colleagues,

We are delighted to be able to invite you to submit a manuscript to the forthcoming Special Issue of the International Journal of Molecular Sciences (IF: 5.6).

This Special Issue is dedicated to an in-depth exploration of the potential applications inherent in mineralized tissue substitutes within the realms of both medicine and dentistry. The primary is to present a meticulous examination of the utilization of bone substitute materials to facilitate the natural emulation of the intricate bone cellular system, with a concerted effort to enhance our comprehension of the underlying bony nanostructure. Particular emphasis is placed on the judicious employment of synthetic biomaterials as bone substitutes, with a specific focus on novel methodologies involving nanomaterials and their rigorous assessment for clinical efficacy. Encompassing a wide array of subjects, including the characterization and application of bone substitute materials, biocompatibility assessment, physicochemical properties, materials in endodontic surgery, and the mechanical and biocompatible properties of CAD/CAM restorative materials, this Issue is poised to make substantial contributions to the scientific discourse surrounding advancements in the application of mineralized tissue substitutes across diverse domains.

This Issue focuses on advancing our understanding of biomaterial applications in the intricate process of bone tissue repair and regeneration. Submitted papers for this Special Issue are expected to present results that delve into molecular-level findings. Emphasis will be placed on exploring the intricate molecular mechanisms involved in the interaction between biomaterials and bone tissues, aiming to contribute valuable insights to the field of bone regeneration research.

Dr. Jacek Matys
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • allograft
  • autograft
  • autologous bone
  • bone
  • graft materials
  • mineral substitutes
  • regeneration

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Published Papers (1 paper)

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Research

21 pages, 3455 KB  
Article
Tocotrienol-Incorporated Gelatin Hydrogel Crosslinked with Genipin for Future Bone Tissue Engineering Applications: Physiochemical Characterization and Biocompatibility
by Alhareth Abdulraheem Al-Ajalein, Nurul ‘Izzah Ibrahim, Mh Busra Fauzi and Norazlina Mohamed
Int. J. Mol. Sci. 2026, 27(4), 1659; https://doi.org/10.3390/ijms27041659 - 8 Feb 2026
Viewed by 376
Abstract
Oral administration of tocotrienol has poor systemic distribution due to poor selectivity by the α-tocopherol transfer protein at the liver. Local injection of tocotrienols with appropriate drug delivery systems is significant to ensure that the drug is delivered directly to the site of [...] Read more.
Oral administration of tocotrienol has poor systemic distribution due to poor selectivity by the α-tocopherol transfer protein at the liver. Local injection of tocotrienols with appropriate drug delivery systems is significant to ensure that the drug is delivered directly to the site of injury or fracture. This paper presents a tocotrienol-loaded gelatin hydrogel crosslinked with genipin for bone regeneration. This innovative method improves the incorporation and sustained delivery of tocotrienol while overcoming its incompatibility with hydrophilic biomaterials. It establishes a novel platform for targeted therapeutic applications in bone treatment. The cytotoxicity and physicochemical properties of tocotrienol were examined using the genipin-crosslinked gelatin hydrogel. A 10% tocotrienol nanoemulsion (TTE) was prepared using a sonicator and characterized with a zeta sizer and FTIR. A dose–response analysis was conducted to determine the appropriate tocotrienol concentration for hydrogel integration with gelatin (7% or 10% w/v) and crosslinked with genipin (0.1% or 0.3% w/v). The dose–response study’s tocotrienol nanoemulsion was added to gelatin before polymerization. With 141.9 nm particles and 0.150 PDI, the nanoemulsion was homogeneous and stable. The 1% tocotrienol nanoemulsion was chosen due to its viability. Formulations 1% TTE_0.1% GNP_7% GEL and 1%TTE_0.3% GNP_7% GEL had superior physicochemical properties compared to other groups. The 1% TTE_0.3% GNP_7% GEL had outstanding hydrophilicity, low weight loss, and a suitable swelling ratio for bone application. SEM scans of the surface and cross-section showed that 1% TTE_0. 3% GNP_7% GEL had interconnected pores with an optimal average pore size of 292 ± 37 μm. Adding tocotrienol to the gelatin hydrogel matrix did not affect FTIR, XRD, or EDX. In vitro cytotoxicity studies indicated >90% cell viability of hFOB 1.19 cells cultured on 1% TTE_0.1% GNP_7% GEL and 1% TTE_0.3% GNP_7% GEL (105 ± 4.36% and 95.36 ± 9.78%). Combining tocotrienol with a genipin-crosslinked gelatin hydrogel demonstrated superior physicochemical properties and no in vitro toxicity. Full article
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