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Horticulturae
Special Issues
Innovative Tissue Culture Techniques for Sustainable Horticulture
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Innovative Tissue Culture Techniques for Sustainable Horticulture

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Propagation and Seeds".

Deadline for manuscript submissions: 25 November 2026 | Viewed by 909

Special Issue Editors

Dr. Rosalia Nuñez Pastrana

E-Mail Website
Guest Editor
Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Amatlán de los Reyes 94945, Mexico
Interests: phytopathogens; plant genetics; plant–pathogen interaction; plant tissue culture
Dr. Carlos Alberto Lecona

E-Mail Website
Guest Editor
Laboratorio de Biotecnología Vegetal, National Technological Institute of Mexico, Tuxtla Gutierrez Campus, Tuxtla Gutiérrez 29050, Mexico
Interests: plant tissue culture; plant biotechnology; agricultural biotechnology; abiotic stress tolerance; genetic improvement; nanotechnology
Dr. Nancy Santana-Buzzy

E-Mail Website
Guest Editor
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Interests: morphogenesis and recalcitrancy; plant tissue culture; micropropagation; conservation; sintetic seed; plant biotechnology; plant breeding

Special Issue Information

Dear Colleagues,

Currently, in order to meet future demands for plant-based food products, to cultivate plant species for ornamental and medicinal purposes, and to reintroduce endangered species, sustainable practices are needed for the propagation, genetic improvement, and conservation of horticultural products. The biological foundations of plant tissue culture allow us to achieve these objectives in a sustainable manner, providing a wide range of techniques that can be used to obtain more productive plants, with greater defense mechanisms against diseases or pests, and with better organoleptic and nutritional characteristics. The use of various plant growth regulators, nanomaterials, biostimulants, and biological agents that exert selective pressure on the explant, as well as plant genetic transformation, allows us to obtain plants with improved qualities in a short time. This Special Issue "Innovative Tissue Culture Techniques for Sustainable Horticulture," aims to present a series of articles to show the advances and innovative in vitro culture techniques in horticultural species using sustainable practices.

Dr. Rosalia Nuñez Pastrana
Dr. Carlos Alberto Lecona
Dr. Nancy Santana-Buzzy
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • sustainability
  • plant tissue culture
  • bioestimulants
  • nanomaterials
  • horticulture

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Published Papers (1 paper)

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Research

17 pages, 5477 KB  
Article
Plant Regeneration via Somatic Embryogenesis in Juglans regia ‘Yunxin No. 14’
by Jinwang Qu, Xiurong Yang, Linhe Xiang, Bolin Wu, Junzan Huang, Chenyang Liang, Aoao Cui, Amenyogbe Mawuli Korsi, Haigang Zhang, Chu Wu, Liping Liu and Xinwu Xiong
Horticulturae 2026, 12(4), 437; https://doi.org/10.3390/horticulturae12040437 - 2 Apr 2026
Viewed by 562
Abstract
The walnut cultivar ‘Yunxin No. 14’ is an early fruiting, high-yielding, and widely adaptable fruit tree with compact growth and superior nuts. Establishing a successful tissue culture system for this cultivar is crucial for its rapid clonal propagation and as a foundation for [...] Read more.
The walnut cultivar ‘Yunxin No. 14’ is an early fruiting, high-yielding, and widely adaptable fruit tree with compact growth and superior nuts. Establishing a successful tissue culture system for this cultivar is crucial for its rapid clonal propagation and as a foundation for future genetic transformation. Using young fruits as explants, 3% NaClO sterilization for 20 min effectively controlled contamination and browning. Somatic embryos induced from zygotic embryos cultured on DKW medium with 30 g·L−1 sucrose showed high proliferation and minimal browning. After a 4-day dehydration treatment using saturated NH4NO3, mature somatic embryos germinated rapidly on differentiation medium (DKW containing 1 mg·L−1 6-BA and 0.1 mg·L−1 IBA), reaching 90.0% germination. Optimal shoot multiplication was achieved on DKW medium supplemented with 2 mg·L−1 6-BA and 0.3 mg·L−1 IBA, yielding a proliferation rate of 91.1% and a proliferation index of 3.1. For rooting, shoots (~3 cm) treated with Clonex® rooting gel were transferred to a low-cost, sugar-free vermiculite medium with gaseous CO2 as the sole carbon source. Root initiation occurred within two weeks at a rate of 54.2%, significantly shortening the rooting phase. Rooted plantlets were acclimatized in a peat:perlite:vermiculite (2:2:1, v/v/v) mixture under high humidity for two weeks before outdoor transfer, achieving an 88.6% survival rate. This study provides a reliable protocol for the micropropagation of ‘Yunxin No. 14’ and a valuable reference for other difficult-to-root woody species. Full article
(This article belongs to the Special Issue Innovative Tissue Culture Techniques for Sustainable Horticulture)
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