Special Issue "MetaGenomics Sequencing In Situ"
Deadline for manuscript submissions: closed (31 August 2020) | Viewed by 81386
Interests: astrobiology; DNA and RNA sequencing; astromaterials; field ecology;
Interests: nanopore sequencing; MinION; infectious disease diagnosis; field ecology; metagenomics; direct RNA sequencing; epigenetics; methylation; long reads; agriculture; biosurveillance
In situ sequencing has a broad range of applications, such as for infectious disease diagnosis and tracking, ecological and agricultural research, and even space exploration, including monitoring crew health and the search for extra-terrestrial life. The MinION sequencer has given the opportunity to improve in situ sequencing as it measures differences in current caused by DNA or RNA strands passing through nanopores embedded in membranes, with the change in current at a given time being diagnostic of the nucleotides passing through the pore. Because nanopore-based sequencing directly measures the molecules rather than relying on their synthesis, it can be used to sequence RNA directly without having to convert it to cDNA first, as well as detect modified bases in both DNA and RNA. The footprint and energy requirements of the MinION is also significantly smaller than other sequencing platforms, enabling use in settings outside of traditional or core laboratories.
In this Special Issue, we seek original research and reviews of field applications of nanopore sequencing, including the development of in situ sample preparation and processing hardware and procedures, as well as local data processing and analysis.
Dr. Aaron S. Burton
Dr. Joseph Russel
Manuscript Submission Information
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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- nanopore sequencing
- infectious disease diagnosis
- field ecology
- direct RNA sequencing
- long reads