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Special Issue "Humanized Yeast Models"
Deadline for manuscript submissions: 15 January 2020.
Dpto. de Microbiología y Parasitología, Universidad Complutense de Madrid. Madrid, Spain.
Interests: Saccharomyces cerevisiae; yeast; signaling; heterologous expression; phosphoinositides; Toll-like receptors (TLR signaling); Phosphatidylinositol 3.kinase (PI3K); Supramolecular organizing complexes (SMOCs)
Laboratory of Cell Death and Cancer Therapy, Department of Molecular Biomedicine, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
Interests: Lipid rafts; cell death; cell death receptors; cell signaling; anticancer drugs; antileishmanial drugs; alkylphospholipid analogs; cancer; neutrophil; Leishmania
Unicellular eukaryotes, like budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe, are consolidated models in basic research. Their genetic tractability and ease of manipulation pushed these microorganisms to a leading position in the post-genomic era, providing key information on the molecular mechanisms that govern conserved cellular modules, pathways and functions, like metabolism, cell division, signaling, vesicle traffic, cytoskeleton, aging, autophagy, etc. Furthermore, the human proteins expressed in these models are often capable of supporting the function or their yeast counterparts. Thus, heterologous expression in yeast is a powerful strategy to address the structure–function relationship in human gene products that may either stand as important drug targets or relate to pathological syndromes.
In the last two decades, multiple humanized yeast models have been developed with various purposes, either by complementing yeast mutants with human genes or by implementing functions or pathways in yeast that are naturally missing in lower eukaryotes. The formation of amyloid bodies or prion fibrils like those produced in neurons in degenerative disorders, the biosynthetic pathway of cholesterol, or oncogenic signaling pathways, to mention some examples, have been successfully reproduced in yeast. In these systems, the yeast cell provides an “in vivo test tube” that allows us to study the behavior of human proteins in the absence of other input characteristic of higher cells, but yet within a cellular environment. Typically, humanized yeast models can be exploited to design bioassays for pharmacological screens in drug discovery or to carry out exhaustive mutational analyses in order to assess protein–protein interactions, mechanistic features of enzymes or diverse functional aspects of the target proteins.
This Special Issue will cover research on the design, implementation or exploitation of yeast-based models to study human proteins, based on their heterologous expression coupled to the genetic versatility of yeast models, with emphasis on those strategies aimed at basic or applied research into genes and proteins related to human pathologies. We encourage researchers working in the field to contribute with original research or review articles.
Prof. Victor J. Cid
Prof. Faustino Mollinedo
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- Heterologous expression
- Saccharomyces cerevisiae
- Cell signaling
- Drug discovery
- Protein–protein interactions
- Microbial models