Advanced Separation Techniques for Food Safety and Functional Component Analysis

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: 10 April 2026 | Viewed by 343

Special Issue Editors


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Guest Editor
Laboratory of Microbial and Enzymes Biotechnology and Biomolecules (LMEBB), Centre of Biotechnology of Sfax (CBS), University of Sfax-Tunisia, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia
Interests: food security; recovery of high value byproducts; phytochemical analysis; natural products
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Guest Editor
Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia
Interests: extraction, separation, and chromatographic identification of bioactive molecules; biological activities in vivo and in vitro

Special Issue Information

Dear Colleagues,

Guaranteeing food safety and categorizing functional components in food products are key aspects of modern food science. In this vein, progressive separation techniques act as a vital part in noticing contaminants, residues, and bioactive compounds with high precision and efficacy. Procedures viz. high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), capillary electrophoresis (CE), and supercritical fluid extraction (SFE) are extensively used for qualitative and quantitative investigations. These tools allow the separation of complex food matrices into their individual components, permitting accurate detection of damaging substances like pesticides, mycotoxins, and heavy metals, as well as beneficial compounds such as antioxidants, vitamins, and peptides. Recent advances, counting microfluidic systems and lab-on-a-chip devices, could provide faster analysis times, minimal sample requirements, and movable applications, making real-time monitoring more accessible. Moreover, joining separation techniques with advanced detection methods like tandem mass spectrometry (MS/MS) develops sensitivity and specificity. The integration of these technologies certifies not only controlling compliance but also assists the expansion of functional foods by consenting targeted extraction and characterization of health-promoting ingredients. As consumer demand for safe, nutritious, and traceable food increases, the application of advanced separation techniques becomes progressively essential in both food safety guarantee and efficient food research. This Special Issue highlights recent advances in separation technologies that are transforming how we detect contaminants and isolate health-promoting components in complex food systems. By showcasing innovative methodologies and their applications, this collection aims to advance research in both food safety assurance and functional food development.

Dr. Slim Smaoui
Dr. Walid Elfalleh
Guest Editors

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Keywords

  • innovative food safety solutions
  • next-generation separation techs
  • precision analysis of bioactive compounds
  • smart detection of food contaminants
  • functional food component profiling
  • high-resolution chromatographic techniques
  • advanced analytical methodologies
  • real-time food quality monitoring
  • green and sustainable extraction methods
  • integrated lab-on-a-chip applications

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Published Papers (1 paper)

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Research

21 pages, 5168 KB  
Article
Pasuchaca (Geranium dielsiaum Knuth): A New Source of Astilbin with Antiglycation Activity
by Guanglei Zuo, Zhaoyang Wu, Hyun-Yong Kim, Jinghui Feng, Soo Kyeong Lee, Yanymee Nimesia Guillen Quispe and Soon Sung Lim
Foods 2025, 14(23), 4167; https://doi.org/10.3390/foods14234167 - 4 Dec 2025
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Abstract
Pasuchaca (Geranium dielsianum Knuth), a traditional Peruvian medicinal plant from the Geraniaceae family used for diabetes management, was investigated for its antiglycative properties. This study aimed to screen, isolate, and identify the active antiglycative compounds from its aerial parts. By coupling a [...] Read more.
Pasuchaca (Geranium dielsianum Knuth), a traditional Peruvian medicinal plant from the Geraniaceae family used for diabetes management, was investigated for its antiglycative properties. This study aimed to screen, isolate, and identify the active antiglycative compounds from its aerial parts. By coupling a methylglyoxal (MGO)-HPLC screening assay with high-speed counter-current chromatography (HSCCC), seven dihydroflavonol derivatives were separated and identified from the 80% methanol extract. The compounds were identified as 2,3-dihydromyricetin 3-O-α-rhamnopyranoside (1), (+)-taxifolin 3-O-β-D-xylopyranoside (2), astilbin (6), isoastilbin (8), 3″-acetyl astilbin (9), and 2″-acetyl astilbin (11). Astilbin was identified as the major constituent, with remarkably high contents of 252.41 mg/g in the 80% methanol extract and 541.04 mg/g in the partitioned upper layer fraction. Astilbin demonstrated potent antiglycation activity across all stages of protein glycation (early, middle, late, and whole stages), significantly surpassing the positive control aminoguanidine. Furthermore, the formation of MGO-astilbin adducts was confirmed by LC-ESI-MS, validating its role as an effective MGO scavenger. This report is the first to isolate these phytochemicals from Pasuchaca. The findings establish astilbin as the key antiglycative component of Pasuchaca, substantiating its traditional use and highlighting its potential as a source of functional food ingredients or natural therapeutics for mitigating glycative stress. Full article
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