Separation and Purification Technology Based on Chromatography of Bioactive Compounds in Food Resources

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Engineering and Technology".

Deadline for manuscript submissions: closed (31 May 2024) | Viewed by 1336

Special Issue Editor


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Guest Editor
Beijing Advanced Innovation Centre for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China
Interests: bioseparation and analytical techniques and their application in natural resources

Special Issue Information

Dear Colleagues,

Separation and purification techniques play important roles in modern food development and manufacture, which can improve the comprehensive utilization of food raw materials, maintain and enhance the nutrition and flavor of food, and improve food safety. Separation and purification technologies are indispensable in the substance-based exploration of functional food, the quantitative structure–activity relationship study of bioactive components, the high-value utilization of agricultural by-products, the extraction of fragrance and aromatic components, pigment extraction and purification, oil extraction, enzyme and vitamin refining, the removal of harmful components, and other fields. With the development of science and technology, an increasing number of separation methods are emerging that are applicable in food research and development. A variety of chromatographic separation techniques are available with high efficiency and flexibility. This Special Issue mainly includes recent studies on novel chromatography-related methods or technologies, as well as new applications in the separation, purification, and findings of bioactive compounds in food-relevant resources.

Prof. Dr. Xueli Cao
Guest Editor

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Keywords

  • liquid chromatography
  • counter-current chromatography
  • convergence chromatography
  • combined chromatography
  • hyphenated and multi-dimensional techniques
  • herbal resources for food
  • agri-food resources
  • food by-products
  • antioxidants
  • phytochemicals
  • botanicals
  • functional food additives (fragrance and pigment)
  • other natural resources and relevant approaches

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Published Papers (1 paper)

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Research

16 pages, 3296 KiB  
Article
Bioassay-Guide Preparative Separation of Hypoglycemic Components from Gynura divaricata (L.) DC by Conventional and pH-Zone Refining Countercurrent Chromatography
by Zetao Shen, Jing Xu, Lijiao Wen, Lu Yin, Xueli Cao, Hairun Pei and Xi Zhao
Foods 2025, 14(4), 578; https://doi.org/10.3390/foods14040578 - 10 Feb 2025
Cited by 1 | Viewed by 718
Abstract
Gynura divaricata (L.) DC is a long-used medicinal and edible plant in China folk. Its hyperglycemic effects have garnered increasing public attention in recent years. This study revealed that the ethyl acetate (EtOAc) and butanol (BuOH) partition fractions of G. divaricata crude extract [...] Read more.
Gynura divaricata (L.) DC is a long-used medicinal and edible plant in China folk. Its hyperglycemic effects have garnered increasing public attention in recent years. This study revealed that the ethyl acetate (EtOAc) and butanol (BuOH) partition fractions of G. divaricata crude extract exhibited significantly higher α-glucosidase inhibition activity and enhanced glucose uptake ability compared to other fractions. Guided by the hypoglycemic bioassay, these two fractions were subjected to isolation of active compounds using high-speed countercurrent chromatography (HSCCC). A two-phase solvent system composed of hexane-methyl tert-butyl ether (MtBE)-methanol-0.1% TFA water was employed for the separation of the EtOAc fraction by conventional HSCCC through a gradient elution strategy. Five major compounds were obtained and identified as chlorogenic acid (1), 3,4-dicaffeoylquinic acid (2), 3,5-dicaffeoylquinic acid (3), 4,5-dicaffeoylquinic acid (4), and kaempferol-3-O-β-D-glucopyranoside (5) by ESI-MS, 1HNMR, and 13CNMR. The chlorogenic acid and the three dicaffeoylquinic acids were found to display higher inhibitory activities against α-glucosidase compared to the flavonoid. Considering their acidic nature, pH-zone-refining CCC (PHZCCC) was then applied for further scale-up separation using a solvent system MtBE: n-butanol: acetonitrile: water with trifluoroacetic acid (TFA) as a retainer and ammonium hydroxide (NH4OH) as an eluter. A significantly higher yield of chlorogenic acid was obtained from the BuOH fraction by PZRCCC. Molecular docking between the caffeoylquinic acids and α-glucosidase confirmed their hypoglycemic activities. This study demonstrates that CCC is a powerful tool for preparative separation of active constituents in natural products. This research presents a novel and effective method for the preparative isolation of hypoglycemic compounds from Gynura divaricata. Full article
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