Germ Cells and Stem Cells in Regeneration and Repair

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: closed (31 March 2022) | Viewed by 10495

Special Issue Editor


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Guest Editor
Mary MacKillop Institute for Health Research, Exercise and Nutrition Research Program, Australian Catholic University, Melbourne, VIC 3000, Australia
Interests: reproductive biology; germ cell differentiation; muscle biology; stem cell and setellite cell differentiation; muscle regenaration and repair; stem cells and ageing

Special Issue Information

Dear Colleagues,

Stem cell therapies hold promise for the treatment of injuries and illnesses associated with a variety of biological and physiological systems, such as the nervous system, the musculoskeletal system, the circulatory system, and the reproductive system. Producing patient-specific cells differentiated from an embryonic, germ, induced pluripotent, tissue-specific and transdifferentiated stem cells in vitro holds the key for these treatments. Although research into this field intensified a couple of decades ago, the mechanisms involved in these cellular modifications are only partially understood. This Special Issue of Cells will explore the mechanisms involved and the laboratory methods used to establish and optimize in vitro models for tissue regeneration and repair with a focus on the role of germ cells and stem cells in regenerative medicine, which may result in greater success in clinical applications.

Dr. Orly Lacham-Kaplan
Guest Editor

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Keywords

  • pluripotent stem cells
  • germ cells
  • transdifferentiated stem cells
  • stem cell therapies
  • tissue regeneration and repair
  • in vitro stem cell differentiation
  • patient specific stem cells

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Published Papers (2 papers)

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Research

12 pages, 1563 KiB  
Article
Lactate Activates Germline and Cleavage Embryo Genes in Mouse Embryonic Stem Cells
by Qing Tian and Li-quan Zhou
Cells 2022, 11(3), 548; https://doi.org/10.3390/cells11030548 - 4 Feb 2022
Cited by 38 | Viewed by 5833
Abstract
Lactate was recently found to mediate histone lysine lactylation and facilitate polarization of M1 macrophages, indicating its role in metabolic regulation of gene expression. During somatic cell reprogramming, lactate promotes histone lactylation of pluripotency genes and improves reprogramming efficiency. However, the function of [...] Read more.
Lactate was recently found to mediate histone lysine lactylation and facilitate polarization of M1 macrophages, indicating its role in metabolic regulation of gene expression. During somatic cell reprogramming, lactate promotes histone lactylation of pluripotency genes and improves reprogramming efficiency. However, the function of lactate in cell fate control in embryonic stem cells (ESCs) remains elusive. In this study, we revealed that lactate supplementation activated germline genes in mouse ESCs. Lactate also induced global upregulation of cleavage embryo genes, such as members of the Zscan4 gene family. Further exploration demonstrated that lactate stimulated H3K18 lactylation accumulation on germline and cleavage embryo genes, which in turn promoted transcriptional elongation. Our findings indicated that lactate supplementation expanded the transcriptional network in mouse ESCs. Full article
(This article belongs to the Special Issue Germ Cells and Stem Cells in Regeneration and Repair)
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12 pages, 2229 KiB  
Article
Effect of Muscle Cell Preservation on Viability and Differentiation of Hamstring Tendon Graft In Vitro
by Jin Kyu Lee, Sungsin Jo, Young Lim Lee, Subin Weon, Jun-Seob Song, Il-Hoon Sung and Tae-Hwan Kim
Cells 2021, 10(4), 740; https://doi.org/10.3390/cells10040740 - 27 Mar 2021
Cited by 4 | Viewed by 3787
Abstract
Muscle tissue is often removed during hamstring tendon graft preparation for anterior cruciate ligament (ACL) reconstruction. The purpose of the study was to test whether preservation of muscle remnants on a tendon graft is beneficial to the graft healing process following ACL reconstruction. [...] Read more.
Muscle tissue is often removed during hamstring tendon graft preparation for anterior cruciate ligament (ACL) reconstruction. The purpose of the study was to test whether preservation of muscle remnants on a tendon graft is beneficial to the graft healing process following ACL reconstruction. Co-culturing of tendon-derived cells (TDCs) and muscle-derived cells (MDCs) was performed at various ratios, and their potential for cell viability and multilineage differentiation was compared to a single TDC cell group. Ligamentous and chondrogenic differentiation was most enhanced when a small population of MDCs was co-cultured with TDCs (6:2 co-culture group). Cell viability and osteogenic differentiation were proportionally enhanced with increasing MDC population size. MDCs co-cultured with TDCs possess both the ability to enhance cell viability and differentiate into other cell lineages. Full article
(This article belongs to the Special Issue Germ Cells and Stem Cells in Regeneration and Repair)
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