Special Issue "Aptamers to Replace Antibodies for in vitro Diagnostics"
Deadline for manuscript submissions: closed (31 December 2020).
Interests: Surface Functionalisation; Development of Cell Biochips, Bacteria Biochips; Cellular Monitoring on-the-chip; Surface treatments ensuring bio-compatibility; Aptamer-based Biochips
Interests: optoelectronic nose/tongue development; aptamer biosensors; surface plasmons resonance imaging; theory of microarrays (DNA or protein); biopolymer conformation; DNA based architectures; soft condensed matter
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Among the different detection modes, the gold standard for biomarkers remains the enzyme-linked immunosorbent assay (ELISA). Its development more than fifty years ago and its sensitivity explain its widespread use in diagnostic applications. In particular, ELISA consists of a sandwich over the target between a target-capturing antibody and a detection antibody. Furthermore, ELISA is generally associated to a secondary antibody to generate an enzymatically amplified signal. However, antibodies as probes convey various drawbacks. In addition to batch-to-batch variations in their production, specific antibodies are tedious, costly, and challenging to generate. Furthermore, their shelf life can limit their incorporation, as embedded reagents in medical devices and their chemical modifications are particularly difficult in order to develop alternatives to the traditional enzymatic amplifications. These problems highlight the need for an alternative to antibodies in order to improve or to bypass ELISA.
Since their discovery in 1990, aptamers have appeared as an ideal alternative to antibodies in diagnostic applications. Aptamers are short oligonucleotides selected from a large library of random sequences for their specificity towards a target (SELEX method). Their 3D conformation confers to aptamers strong sensitivity and selectivity. In vitro selection allows the recognition of a large variety of targets ranging from ions, small molecules, proteins up to cells. The large-scale in vitro synthesis of aptamers ensures the low-cost production of the future reagents with a large set of possible chemical modifications of sequences. Finally, their chemical stability provides numerous opportunities for their integration in point-of-care medical devices such as paper strips or microfluidic cartridges.
Despite all those advantages, up to now, aptamers have failed to replace antibodies. The aim of this Special Issue is to provide a state-of-the-art situation of the developments of aptamers as probes, the detection modes associated for in vitro diagnostics, and their integration in medical devices. Papers addressing the remaining challenges and hurdles still to overcome in order to access the IVD market are also welcome.
Dr. Yoann Roupioz
Dr. Arnaud Buhot
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- in vitro diagnostics (IVD)
- medical devices