Label-Free Electrochemical Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 1 June 2026 | Viewed by 2394

Special Issue Editors


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Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, Araras 13600-970, SP, Brazil
Interests: disposable sensors; 3D-printed electrodes; nanomaterials
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, Brazil
Interests: biosensor; adiponectin; synthetic biology; biosensor and diabetes
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Label-free biosensors represent a groundbreaking advancement in the field of biosensing, offering significant potential for real-time, sensitive, and efficient detection without the need for traditional labeling or markers. This Special Issue in Biosensors aims to highlight the latest developments, applications, and challenges surrounding label-free biosensing technologies. These biosensors operate through a variety of mechanisms, such as optical, electrochemical, and piezoelectric sensing, enabling the rapid detection of biological interactions, biomolecules, and pathogens with minimal sample preparation. The importance of label-free biosensors is underscored by their ability to simplify assay workflows, reduce costs, and increase sensitivity, especially in fields such as medical diagnostics, environmental monitoring, and drug development. The articles within this Special Issue delve into cutting-edge innovations, exploring novel materials, sensor architectures, and analytical techniques that push the boundaries of label-free detection. Collectively, this Special Issue provides a comprehensive view of the current state of label-free biosensors, their transformative potential, and future directions for further research and commercialization.

Prof. Dr. Bruno Campos Janegitz
Prof. Dr. Laís Canniatti Brazaca
Guest Editors

Manuscript Submission Information

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Keywords

  • label-free detection
  • nanomaterials in biosensing
  • biomolecular interaction
  • medical diagnostics
  • environmental monitoring
  • point-of-care devices

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Published Papers (2 papers)

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Review

33 pages, 2694 KB  
Review
Label-Free Electrochemical Biosensors: An Updated Perspective Focused on Genosensing, Multiplexing, and Commercial Potential
by Jefferson H. S. Carvalho, Marcus A. S. Catai, Lucas V. Bertolim, Rafaela C. Freitas, Jessica R. Camargo, Laís C. Brazaca and Bruno C. Janegitz
Biosensors 2026, 16(2), 98; https://doi.org/10.3390/bios16020098 - 4 Feb 2026
Viewed by 990
Abstract
The increasing impact of infectious, cardiovascular and neurodegenerative diseases has intensified the demand for early and decentralized diagnostics. Label-free electrochemical biosensors are promising candidates, offering high sensitivity, low reagent consumption and miniaturizable, low-cost architectures for point-of-care (PoC) testing. This review summarizes advances in [...] Read more.
The increasing impact of infectious, cardiovascular and neurodegenerative diseases has intensified the demand for early and decentralized diagnostics. Label-free electrochemical biosensors are promising candidates, offering high sensitivity, low reagent consumption and miniaturizable, low-cost architectures for point-of-care (PoC) testing. This review summarizes advances in immobilization strategies, recognition elements such as DNA, antibodies, aptamers, and molecularly imprinted polymers, as well as electrode platforms including glassy carbon, screen-printed, and 3D-printed systems, with an emphasis on DNA biosensors, multiplexed configurations, and applications to disease biomarkers. Beyond analytical performance, we critically examine the barriers that keep most devices at the proof-of-concept stage, including bioreceptor stability and immobilization, limited validation in real samples, reliance on conventional materials, challenges in scalable manufacturing, transport, and storage, and the absence of fully integrated PoC systems. Finally, we discuss significant advances in sensitivity, reproducibility, and application to real samples, but note that translation to real-world use and commercialization remains limited. Full article
(This article belongs to the Special Issue Label-Free Electrochemical Biosensing)
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28 pages, 5430 KB  
Review
Electrochemical Sensors as a Tool for Taste Perception in Pharmaceutical Products: Advances and Perspectives
by Juliana Luz Melo Gongoni, Marilia Medeiros, Hatylas Azevedo and Margarete Moreno de Araújo
Biosensors 2026, 16(2), 84; https://doi.org/10.3390/bios16020084 - 30 Jan 2026
Viewed by 1016
Abstract
Taste masking in pharmaceutical products is a complex and subjective process that requires reliable evaluation methods. This review focuses on the electronic tongue (e-tongue), an emerging sensor-based technology designed to mimic human taste perception without the need for human panels. E-tongue systems provide [...] Read more.
Taste masking in pharmaceutical products is a complex and subjective process that requires reliable evaluation methods. This review focuses on the electronic tongue (e-tongue), an emerging sensor-based technology designed to mimic human taste perception without the need for human panels. E-tongue systems provide objective data to support the development of palatable formulations. In this review, we discuss the principles, types of e-tongue devices, data processing approaches, and their applications in pharmaceutical research. By comparing e-tongue performance with human taste assessment, we highlight its potential as a complementary tool to traditional in vitro assays, accelerating formulation development and improving patient adherence. Full article
(This article belongs to the Special Issue Label-Free Electrochemical Biosensing)
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