Special Issue "Biosensors, Bioinstrumentation and BioMEMS: Special Issue after the SEIA' 2021 Conference"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 March 2022) | Viewed by 2907

Special Issue Editor

International Frequency Sensor Association (IFSA), Castelldefels, 08860 Barcelona, Spain
Interests: smart sensors; digital sensors; artificial intelligence-based sensors; frequency measurements; frequency-to-digital converters
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The 7th Annual International Conference on Sensors and Electronic Instrumentation Advances (SEIA' 2021) is a forum for the presentation, discussion, and exchange of information and latest research and development results in both theoretical and experimental research in sensors, transducers, and their related fields. It brings together researchers, developers, and practitioners from diverse fields, including international scientists and engineers from academia, research institutes, and companies to present and discuss the latest results in the field of sensors and measurements. 

The SEIA conference focuses on any significant breakthrough and innovation in Sensors, Electronics, Measuring Instrumentation, and Transducer Engineering Advances and their applications. The main aim of the SEIA conference is to find solutions to the aforementioned restraining factors. 

The authors of conference papers falling within the scope of Biosensors at this conference are invited to submit extended versions to this Special Issue for publication. 

While the Special Issue will preferably provide extended versions of the original works selected for the conference SEIA' 2021, it will not be strictly restricted to these, as new papers related to the conference themes are also welcome.

Prof. Dr. Sergey Y. Yurish
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

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18 pages, 4160 KiB  
Detection of a Double-Stranded MGMT Gene Using Electrochemically Reduced Graphene Oxide (ErGO) Electrodes Decorated with AuNPs and Peptide Nucleic Acids (PNA)
Biosensors 2022, 12(2), 98; https://doi.org/10.3390/bios12020098 - 05 Feb 2022
Cited by 3 | Viewed by 1931
The ability to detect double-stranded DNA (dsDNA) as a biomarker without denaturing it to single-stranded DNA (ss-DNA) continues to be a major challenge. In this work, we report a sandwich biosensor for the detection of the ds-methylated MGMT gene, a potential biomarker for [...] Read more.
The ability to detect double-stranded DNA (dsDNA) as a biomarker without denaturing it to single-stranded DNA (ss-DNA) continues to be a major challenge. In this work, we report a sandwich biosensor for the detection of the ds-methylated MGMT gene, a potential biomarker for brain tumors and breast cancer. The purpose of this biosensor is to achieve simultaneous recognition of the gene sequence, as well as the presence of methylation. The biosensor is based on reduced graphene oxide (rGO) electrodes decorated with gold nanoparticles (AuNPs) and uses Peptide Nucleic Acid (PNA) that binds to the ds-MGMT gene. The reduction of GO was performed in two ways: electrochemically (ErGO) and thermally (TrGO). XPS and Raman spectroscopy, as well as voltammetry techniques, showed that the ErGO was more efficiently reduced, had a higher C/O ratio, showed a smaller crystallite size of the sp2 lattice, and was more stable during measurement. It was also revealed that the electro-deposition of the AuNPs was more successful on the ErGO surface due to the higher At% of Au on the ErGO electrode. Therefore, the ErGO/AuNPs electrode was used to develop biosensors to detect the ds-MGMT gene. PNA, which acts as a bio-recognition element, was used to form a self-assembled monolayer (SAM) on the ErGO/AuNPs surface via the amine-AuNPs interaction, recognizing the ds-MGMT gene sequence by its invasion of the double-stranded DNA and the formation of a triple helix. The methylation was then detected using biotinylated-anti-5mC, which was then measured using the amperometric technique. The selectivity study showed that the proposed biosensor was able to distinguish between blank, non-methylated, non-complementary, and target dsDNA spiked in mouse plasma. The LOD was calculated to be 0.86 pM with a wide linear range of 1 pM to 50 µM. To the best of our knowledge, this is the first report on using PNA to detect ds-methylated DNA. This sandwich design can be modified to detect other methylated genes, making it a promising platform to detect ds-methylated biomarkers. Full article
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