Emerging Materials for Biosensing in Nano/Microfluidics

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 1164

Editors


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Guest Editor

E-Mail Website
Guest Editor
Institute of Materials Science of Barcelona (ICMAB-CSIC), Campus UAB, 08193 Bellaterra, Spain
Interests: materials science; inorganic chemistry; nanotechnology; biochemistry; nanomedicine

Special Issue Information

Dear Colleagues,

The integration of nano/microfluidics with advanced biosensing technologies has revolutionized diagnostic and analytical applications, offering unparalleled capabilities for the manipulation and analysis of miniscule fluid volumes and biological targets. A pivotal driver of this progress is the development and application of novel functional materials. The development of biosensors involves the emergence of novel detection techniques, and their integration with high-affinity biomolecules facilitates the precise detection of a wide array of analytes. This Special Issue aims to spotlight the recent emergence of key material classes that are significantly enhancing the performance of biosensors within microfluidic platforms. We eagerly welcome submissions that showcase the forefront of developments in this research field. Topics may include, but are not limited to, the following: carbon-based nanomaterials (such as graphene and carbon nanotubes) for their exceptional electrical conductivity and large surface area; metallic and metal-oxide nanoparticles (like gold and quantum dots) for their superior optical and catalytic properties; and various polymers and hydrogels that provide tailored biocompatibility and stimulus-responsive behavior. Furthermore, the exploration of molecularly imprinted polymers (MIPs) and novel two-dimensional (2D) materials beyond graphene is expanding the frontiers of specificity and sensitivity. The synergy between these engineered materials and the inherent advantages of microfluidics—such as low sample consumption, rapid analysis, and high integration—enables the creation of highly sensitive, selective, and portable biosensing devices.

Prof. Dr. Shunbo Li
Dr. Muling Zeng
Guest Editors

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Keywords

  • biosensors
  • nanotechnology
  • micro/nanofluidics
  • wearable devices
  • artificial intelligence
  • nanomaterials

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Published Papers (1 paper)

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Research

12 pages, 6932 KB  
Article
DNA Electrochemical Sensor Based on Exonuclease III-Assisted Cycling Signal Amplification for Ultrasensitive Detection of Genetically Modified Soybean
by Lidan Niu, Siyu Huang, Jinmei Zhao, Wenjing Yang, Zhengnan Li, Siqi Niu, Jianchun Yang, Shiqi Chen and Qihui Wang
Biosensors 2026, 16(5), 279; https://doi.org/10.3390/bios16050279 - 11 May 2026
Viewed by 765
Abstract
The safety of genetically modified crops, particularly the commercial cultivation of glyphosate-resistant genetically modified soybeans, has given rise to significant public concern. Consequently, there is an urgent need to develop efficient and precise methods for detecting genetically modified components. The present study constructed [...] Read more.
The safety of genetically modified crops, particularly the commercial cultivation of glyphosate-resistant genetically modified soybeans, has given rise to significant public concern. Consequently, there is an urgent need to develop efficient and precise methods for detecting genetically modified components. The present study constructed a novel electrochemical biosensor based on nucleic acid exonuclease III (Exo III)-assisted cyclic signal amplification and hairpin probe recognition for the highly sensitive and specific detection of the CP4-EPSPS gene in genetically modified soybeans. The sensor achieves exponential signal amplification by triggering Exo III to cyclically cleave the hairpin probe (H1) upon target DNA binding. Subsequent to this, the released DNA fragments hybridize with the methylene blue-labeled signal probe (HS-MB) that has been immobilized on the electrode surface. This process induces conformational changes and a decrease in the current signal, thereby enabling quantitative analysis of the target gene. The experimental phase of the study successfully validated the sensor’s mechanism and systematically optimized key parameters such as Exo III concentration and reaction time. In optimal conditions, the sensor demonstrated excellent linearity with target DNA concentrations ranging from 100 fM to 10 nM, achieving a detection limit as low as 0.1072 pM. Furthermore, it exhibited remarkable repeatability and stability. This study provides an analytical tool with broad application prospects for the rapid and precise detection of genetically modified crops. Full article
(This article belongs to the Special Issue Emerging Materials for Biosensing in Nano/Microfluidics)
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