Advances in Metabolomics in Health and Disease

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Molecular Medicine".

Deadline for manuscript submissions: 31 May 2026 | Viewed by 2719

Special Issue Editor


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Guest Editor
Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
Interests: food chemistry; infrared spectroscopy; food biochemistry; food science; plant physiology; antioxidants; polyphenols; anthocyanins; high-pressure liquid chromatography
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Special Issue Information

Dear Colleagues,

Metabolomics represents an emerging area of research, as a comprehensive technology that focuses on small molecules involved in different metabolic pathways of living organisms, from microorganisms, to plants, animals and humans. It reflects an organism’s phenotype through a global comprehensive analysis of metabolite profiles (untargeted metabolic state of a biological system, e.g., tissue, blood, urine, saliva, cell cultures) or quantification of specific metabolites, as part of the metabolome (targeted approach). It addresses the interconnections with genomics, proteomics and epigenetics, including microbiome and environmental factors. It may include experimental in vitro or in vivo studies in plant or animal models, as well as preclinical and clinical research in human diseases. Metabolomics takes advantage of high-throughput techniques (mass spectrometry or nuclear magnetic resonance coupled or not with high-performance liquid or gas chromatography) for the screening of numerous molecules. The large-scale data generated are complemented by machine learning bioinformatics, in order to recognize patterns and build models that are utilized for the classification, regression, or clustering the complex metabolomic data. These may unravel the specific physiological and pathological mechanisms related to the systems biology approach and personalized medicine.  It offers possibilities to find biomarkers of diagnosis, which are critical for precision health approaches and improved diagnostics, with an impact on clinical decisions.

This Special Issue “Advances in Metabolomics in Health and Disease” encourages the submission of original metabolomics-based studies, narrative or systematic reviews. This Special Issue may offer the opportunity for authors to demonstrate the impact of metabolomics on the diagnosis and prognosis of different diseases using either human samples or animal models, but also to integrate metabolomics into routine healthcare to enable preventive and personalized approaches.

Prof. Dr. Carmen Socaciu
Guest Editor

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Keywords

  • metabolomics
  • disease biomarkers
  • metabolic phenotyping
  • metabolic pathways
  • mass spectrometry
  • NMR spectroscopy
  • metabolic databases
  • bioinformatics

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Published Papers (2 papers)

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Review

19 pages, 1217 KB  
Review
Beyond Bulk Metabolomics: Emerging Technologies for Defining Cell-Type Specific Metabolic Pathways in Health and Disease
by Yichen Gong and Samuel Weinberg
Biomolecules 2025, 15(12), 1687; https://doi.org/10.3390/biom15121687 - 2 Dec 2025
Viewed by 863
Abstract
While metabolomics has emerged as a powerful tool for discovering disease biomarkers, the clinical utility of plasma or tissue metabolite profiles remains limited due to metabolic heterogeneity and flexibility across cell types. Traditional bulk metabolomics fails to capture the distinct metabolic programs operating [...] Read more.
While metabolomics has emerged as a powerful tool for discovering disease biomarkers, the clinical utility of plasma or tissue metabolite profiles remains limited due to metabolic heterogeneity and flexibility across cell types. Traditional bulk metabolomics fails to capture the distinct metabolic programs operating within rare cell populations that often drive disease pathogenesis. This review examines cutting-edge approaches that overcome these limitations by characterizing metabolism at single-cell and cell-type-specific resolution, with particular emphasis on rare immune cell populations as a proof of concept. We discuss how the integration of flow cytometric metabolic profiling, molecular techniques, advanced metabolomics platforms, and computational modeling enables unprecedented insight into cell-intrinsic metabolic states within physiological contexts. We critically evaluate how these technologies reveal metabolic plasticity that confounds bulk measurements while identifying cell-type-specific metabolic vulnerabilities. Finally, we address the crucial challenge of establishing causality in metabolic pathways, a prerequisite for translating metabolomic discoveries into clinically actionable interventions. By moving beyond descriptive metabolomics toward a mechanistic understanding of cell-type-specific metabolism, these approaches promise to deliver the precision required for effective metabolic targeting in disease. Full article
(This article belongs to the Special Issue Advances in Metabolomics in Health and Disease)
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21 pages, 1190 KB  
Review
Glycerol Kinase 2 as a Metabolic Sentinel for Human Sperm Motility and Male Fertility
by João S. Oliveira, Rúben J. Moreira, Ana D. Martins, Marco G. Alves and Pedro F. Oliveira
Biomolecules 2025, 15(9), 1249; https://doi.org/10.3390/biom15091249 - 29 Aug 2025
Viewed by 1530
Abstract
Male infertility affects 8–12% of couples worldwide and is solely responsible in up to 30% of cases. Assisted Reproductive Technologies (ARTs) provide potential solutions, particularly in conditions where spermatozoa display structural abnormalities or impaired motility, such as asthenozoospermia. Sperm metabolism demonstrates remarkable flexibility, [...] Read more.
Male infertility affects 8–12% of couples worldwide and is solely responsible in up to 30% of cases. Assisted Reproductive Technologies (ARTs) provide potential solutions, particularly in conditions where spermatozoa display structural abnormalities or impaired motility, such as asthenozoospermia. Sperm metabolism demonstrates remarkable flexibility, shifting between glycolysis and oxidative phosphorylation to produce ATP required for motility. Glycerol kinase 2 (GK2) phosphorylates glycerol in the sperm midpiece, generating glycerol-3-phosphate, a key intermediate in glycolysis, lipid metabolism, and oxidative phosphorylation. The localization of GK2 suggests not only a regulatory role in sperm metabolism but also a possible association with VDAC proteins, contributing to ADP-ATP exchange between the cytosol and mitochondria. Elucidating the role of GK2 in spermatozoa is of particular relevance, as this enzyme not only contributes to key metabolic pathways but may also interact with VDAC proteins, influencing mitochondrial function and energy exchange. Such interactions could play a pivotal role in regulating sperm motility. A deeper understanding of these mechanisms could position GK2 as a valuable biomarker: in scenarios where GK2–VDAC interactions are confirmed, it may guide optimized sperm selection methods in ARTs, whereas the absence or impairment of such interactions could serve as a diagnostic indicator in asthenozoospermic men. Full article
(This article belongs to the Special Issue Advances in Metabolomics in Health and Disease)
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