Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.7
3.6
Journals
Biology
Special Issues
Research on Enzyme Activities and New Advances on Their Regulation
share announcement

Research on Enzyme Activities and New Advances on Their Regulation

A special issue of Biology (ISSN 2079-7737).

Deadline for manuscript submissions: closed (15 May 2022) | Viewed by 8844

Special Issue Editor

Dr. Roberta Moschini

E-Mail Website
Guest Editor
Department of Biology, University of Pisa, Via S. Zeno 51, 56127 Pisa, Italy
Interests: enzyme kinetic; enzyme inhibition assays; nutraceuticals; natural or synthetic bioactive compounds; proteins purification; oxidative stress; antioxidants; cellular biochemistry
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

enzymes are unique for cell life and their study represent a relevant aspect in modern biology going from the clarification of their role in both cellular metabolism and diseases to their biotechnological applications. The study of the regulation and modulation of enzyme activities has a key role in different biological aspects, not only for pharmacological applications but also for a better knowledge of the role of enzymes in the main cellular metabolic pathways.  Thus, structural and functional characterization of enzymes is fundamental in order to define the most efficient molecules for the modulation of enzyme activity.

 Aim of the issue is to present reviews or research articles addressed to broaden the knowledge of enzymes in both structural and functional terms. The articles will focus on various aspects of enzyme study, from enzyme kinetics to their structural characterization and their application in both pharmacological and biotechnological fields.

Dr. Roberta Moschini
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Keywords: enzyme structure
  • catalytic mechanism
  • enzyme inhibition
  • natural or synthetic bioactive compounds
  • enzyme regulation
  • protein engineering
  • molecular pathology
  • drug design
  • molecular disease

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (2 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

11 pages, 1882 KiB  
Article
Biostimulatory Effects of Amino Acids on Phenylalanine Ammonia Lyase, Capsaicin Synthase, and Peroxidase Activities in Capsicum baccatum L.
by Tilen Zamljen, Aljaz Medic, Metka Hudina, Robert Veberic and Ana Slatnar
Biology 2022, 11(5), 674; https://doi.org/10.3390/biology11050674 - 27 Apr 2022
Cited by 9 | Viewed by 2471
Abstract
Biostimulants are widely used in agriculture because they can improve fruit quality and quantity. Less is known about how biostimulants act over time in plants, in our case peppers, and how they affect the enzyme activity of important enzymes for capsaicinoid synthesis. The [...] Read more.
Biostimulants are widely used in agriculture because they can improve fruit quality and quantity. Less is known about how biostimulants act over time in plants, in our case peppers, and how they affect the enzyme activity of important enzymes for capsaicinoid synthesis. The biostimulatory effects of amino acids on the activities of phenylalanine ammonia lyase (PAL), capsaicin synthase (CS), and peroxidase (POX) were investigated in the pericarp and placenta of the chili pepper Capsicum baccatum L. cv. “Bishop Crown” over 72 h of application. The PAL and CS activities significantly increased in the placenta after 1 h of biostimulant application, with significant increases of 130% and 16%, respectively. The POX activity remained unchanged over the full 72 h in the placenta but significantly increased after 48 h in the pericarp (+53%). Total capsaicinoids increased in the first hour of biostimulant application, by 4.30 g/kg FW in the placenta (19%) and by 0.94 g/kg FW in the pericarp (+56%). Biostimulant application also increased total and individual capsaicinoids after 48 h in the chili placenta and pericarp. With improved methods for enzymatic determination, we gained new insights into the responses of chilies to biostimulant amino acids. Full article
(This article belongs to the Special Issue Research on Enzyme Activities and New Advances on Their Regulation)
►▼ Show Figures

Graphical abstract

16 pages, 17594 KiB  
Article
Effects of A6E Mutation on Protein Expression and Supramolecular Assembly of Yeast Asparagine Synthetase
by Thunyarat Surasiang and Chalongrat Noree
Biology 2021, 10(4), 294; https://doi.org/10.3390/biology10040294 - 3 Apr 2021
Cited by 1 | Viewed by 3858
Abstract
Asparagine synthetase deficiency (ASD) has been found to be caused by certain mutations in the gene encoding human asparagine synthetase (ASNS). Among reported mutations, A6E mutation showed the greatest reduction in ASNS abundance. However, the effect of A6E mutation has not yet been [...] Read more.
Asparagine synthetase deficiency (ASD) has been found to be caused by certain mutations in the gene encoding human asparagine synthetase (ASNS). Among reported mutations, A6E mutation showed the greatest reduction in ASNS abundance. However, the effect of A6E mutation has not yet been tested with yeast asparagine synthetase (Asn1/2p). Here, we constructed a yeast strain by deleting ASN2 from its genome, introducing the A6E mutation codon to ASN1, along with GFP downstream of ASN1. Our mutant yeast construct showed a noticeable decrease of Asn1p(A6E)-GFP levels as compared to the control yeast expressing Asn1p(WT)-GFP. At the stationary phase, the A6E mutation also markedly lowered the assembly frequency of the enzyme. In contrast to Asn1p(WT)-GFP, Asn1p(A6E)-GFP was insensitive to changes in the intracellular energy levels upon treatment with sodium azide during the log phase or fresh glucose at the stationary phase. Our study has confirmed that the effect of A6E mutation on protein expression levels of asparagine synthetase is common in both unicellular and multicellular eukaryotes, suggesting that yeast could be a model of ASD. Furthermore, A6E mutation could be introduced to the ASNS gene of acute lymphoblastic leukemia patients to inhibit the upregulation of ASNS by cancer cells, reducing the risk of developing resistance to the asparaginase treatment. Full article
(This article belongs to the Special Issue Research on Enzyme Activities and New Advances on Their Regulation)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-2
Back to TopTop