Molecular Biology of Plants

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: 1 July 2025 | Viewed by 1206

Special Issue Editor

Forestry College, Guangxi University, Nanning 530000, China
Interests: secondary metabolism and stress resistance in forestry plants; anthocyanin; shoot branching

Special Issue Information

Dear Colleagues,

With the fast development of modern genomic and molecular technologies, targeted molecular breeding of both herbaceous and forestry species has become available. The production of high-quality, high-yield, and high-stress-resistant cultivars relies on a deep understanding of molecular mechanisms underlying the formation of important agronomic traits, the identification and functional characterization of novel genes, and efficient genetic modification technologies. The mining of yield- and stress-resistance-related genes and their functional identification by using multi-omics, molecular, and transgenic strategies provides the basic scientific support for the clarification of the molecular mechanisms and genetic improvement. This Special Issue will address a selection of recent research topics and current review articles in the field of plant molecular biology, genetics, genomics, metabolomics, and genetic modification technologies. Examples of research topics that will be examined in this Special Issue include the following: application of next-generation sequencing for genetic improvement, multi-omics-based gene mining, novel approaches to germplasm utilization, mapping and cloning of genes, development of tools for marker-assisted selection, molecular basis of biotic and abiotic stress tolerance, metabolomes and stress tolerance, novel transgenic technologies, etc.

Dr. Jun Ni
Guest Editor

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Keywords

  • transcriptome
  • metabolome
  • genome editing
  • genetic modification
  • stress resistance
  • yield improvement
  • secondary metabolites
  • flowering

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Published Papers (1 paper)

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Research

16 pages, 3843 KiB  
Article
Optimal Vase Solution for Gerbera hybrida Cut Flower Keeping Fresh by Activating SA and Cytokinin Signaling and Scavenging Reactive Oxygen Species
by Chaoshui Xia, Yiyang Cao, Weixin Gan, Huifeng Lin, Huayang Li, Fazhuang Lin, Zhenhong Lu and Weiting Chen
Biology 2025, 14(1), 18; https://doi.org/10.3390/biology14010018 - 28 Dec 2024
Viewed by 888
Abstract
Gerbera (Gerbera hybrida) is a popular cut flower on the market, so extending its vase life (VL) is an important goal in the horticultural industry. The aim of this study was to improve the freshness of gerbera cut flowers through the [...] Read more.
Gerbera (Gerbera hybrida) is a popular cut flower on the market, so extending its vase life (VL) is an important goal in the horticultural industry. The aim of this study was to improve the freshness of gerbera cut flowers through the optimal solution (OS) and to analyze its preservation mechanism. We used chitosan (COS), calcium chloride (CaCl2), and citric acid (CA) as the main ingredients of the vase solution and determined the OS ratio of 104 mg/L of COS, 92 mg/L of CA, and 93 mg/L of CaCl2 using the Box–Behnken design-response surface method (BBD-RSM). Gerbera preservation results showed that the VL of the OS was 14.5 days, which was significantly longer than that of flowers maintained in the Basic Vase Solution (BVS) and the Commercial Formulation (CF) and was highly consistent with the theoretical VL of 14.57 d. Transcriptome analysis indicated that the OS might extend VL by regulating phytohormone signaling pathways, such as cytokinin and salicylic acid signaling. The qRT-PCR analysis of key candidate genes supported these findings, with significant upregulation observed in genes related to cytokinin synthesis (e.g., GhIPT1 and GhIPT9), salicylic acid signaling related to pathogen defense (e.g., GhTGA1, GhTGA4, GhNPR1, and GhRBOHA), and plant wax synthesis and stress response (e.g., GhKCS5, GhCUT1, and GhKCS6). Further, transcriptome GO-enrichment and physiological analysis showed that the OS might extend VL of Gerbera cut flowers by scavenging reactive oxygen species, including by activating the expression of genes related to oxidoreductase activity and the activities of antioxidant-system-related enzymes catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and superoxide dismutase (SOD), while decreasing the malondialdehyde (MDA) content. These results provide valuable insights into the mechanisms underlying the extended VL of gerbera cut flowers and offer a foundation for developing more effective preservation techniques. Full article
(This article belongs to the Special Issue Molecular Biology of Plants)
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