Metabolism of Industrial Microorganisms

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Microbiology".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 1846

Special Issue Editors


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Guest Editor
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Interests: microbial metabolic engineering; synthetic biology; applied microbiology; pseudomonas; biocides; biocontrol; natural products

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Guest Editor
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Interests: yeast; synthetic biology; metabolic engineering; biofuels; organic acids; genome mining
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Interests: industrial microbiology; biofuels; biochemicals production

Special Issue Information

Dear Colleagues,

Microbial fermentation processes have been used extensively in the production of various foods, bio-based chemicals, biopharmaceuticals, biofuels and other commercial products, various types of microorganisms are used for large-scale production in microbial fermentation, including natural mutant strains and genetically engineered strains of Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis, Actinomycetes, Aspergillus niger, etc. These industrial microorganisms possess a broad variety of enzymes to make different bioconversions possible, and they also have relatively high metabolic activities that permit a series of biotransformation or bioreactions to take place rapidly. It is hoped that industrial microorganisms could grow quickly on media made of cheap raw materials, synthesize the target products rapidly and efficiently, be easy to maintain and cultivate to ensure the stability of fermentation production and product quality, and should have genetic stability with infrequent mutations.

To improve industrial production, the metabolic mechanism, metabolic pathway and metabolic regulation of industrial microorganisms should be clarified in depth. Microbial metabolic engineering and microbial-based synthetic biology are powerful and eco-friendly approaches for the production of high-value products from sustainable carbon sources. The performance of industrial strains can be improved by constructing efficient synthetic pathways, regulating the metabolic networks, enhancing pathway metabolic fluxes, reforming medium composition, cofactor and precursor supply, carrying out directed evolution to improve enzyme activity, etc. With the continuous progress and application of metabolic engineering, multi-omics technology, synthetic biology and other methods, the metabolic mechanism of industrial microorganisms will be clearly revealed, and the microbial cell factories will realize green production with high efficiency.

The scope of this Special Issue is to address the potential and challenges to improve the metabolic ability of various industrial microorganisms. This Special Issue will cover topics related to the microbial metabolic engineering, microbial fermentation, metabolic regulation, metabolomics, metabolic flux analysis, design and construction of metabolic pathway, metabolic network and microbial cell factory. These studies will help people make use of various metabolic research strategies, fully achieve the metabolic potential of industrial microbial strains.

Prof. Dr. Xuehong Zhang
Prof. Dr. Xin-Qing Zhao
Dr. Kai Li
Guest Editors

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Keywords

  • microbial metabolic engineering
  • microbial fermentation
  • industrial microorganisms
  • microbial cell factory
  • metabolic regulation
  • metabolic network
  • metabolic flux analysis
  • metabolic pathway
  • metabolomics
  • synthetic biology
  • applied microbiology
  • industrial production
  • biofuels and biochemicals
  • bioactive compounds

Published Papers (1 paper)

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Research

10 pages, 1744 KiB  
Article
Economical Production of Phenazine-1-carboxylic Acid from Glycerol by Pseudomonas chlororaphis Using Cost-Effective Minimal Medium
by Yu-Xuan Li, Sheng-Jie Yue, Yi-Fan Zheng, Peng Huang, Yan-Fang Nie, Xiang-Rui Hao, Hong-Yan Zhang, Wei Wang, Hong-Bo Hu and Xue-Hong Zhang
Biology 2023, 12(10), 1292; https://doi.org/10.3390/biology12101292 - 27 Sep 2023
Viewed by 1060
Abstract
Phenazine compounds are widely used in agricultural control and the medicine industry due to their high inhibitory activity against pathogens and antitumor activity. The green and sustainable method of synthesizing phenazine compounds through microbial fermentation often requires a complex culture medium containing tryptone [...] Read more.
Phenazine compounds are widely used in agricultural control and the medicine industry due to their high inhibitory activity against pathogens and antitumor activity. The green and sustainable method of synthesizing phenazine compounds through microbial fermentation often requires a complex culture medium containing tryptone and yeast extract, and its cost is relatively high, which greatly limits the large-scale industrial production of phenazine compounds by fermentation. The aim of this study was to develop a cost-effective minimal medium for the efficient synthesis of phenazine compounds by Pseudomonas chlororaphis. Through testing the minimum medium commonly used by Pseudomonas, an ME medium for P. chlororaphis with a high production of phenazine compounds was obtained. Then, the components of the ME medium and the other medium were compared and replaced to verify the beneficial promoting effect of Fe2+ and NH4+ on phenazine compounds. A cost-effective general defined medium (GDM) using glycerol as the sole carbon source was obtained by optimizing the composition of the ME medium. Using the GDM, the production of phenazine compounds by P. chlororaphis reached 1073.5 mg/L, which was 1.3 times that achieved using a complex medium, while the cost of the GDM was only 10% that of a complex medium (e.g., the KB medium). Finally, by engineering the glycerol metabolic pathway, the titer of phenazine-1-carboxylic acid reached the highest level achieved using a minimum medium so far. This work demonstrates how we systematically analyzed and optimized the composition of the medium and integrated a metabolic engineering method to obtain the most cost-effective fermentation strategy. Full article
(This article belongs to the Special Issue Metabolism of Industrial Microorganisms)
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