Development and Utilization of Yeast Resources, 2nd Edition

A special issue of Journal of Fungi (ISSN 2309-608X).

Deadline for manuscript submissions: 31 December 2025 | Viewed by 2341

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Guest Editor
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Interests: yeast; synthetic biology; metabolic engineering; biofuels; organic acids; genome mining
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Special Issue Information

Dear Colleagues,

Yeasts are simple and single cellular fungi that are widely distributed and have been widely used in various traditional industries. Yeasts are also being investigated as microbial cell factories in novel applications in medicine, agriculture, and biorefinery of lignocellulosic biomass. Among various yeasts, budding yeast is efficient to ferment sugars into alcohol, and has been used in the production of wine, beer, beverage and biofuels. In recent years, non-conventional yeasts, such as Yarrowia lipolytica, Kluyveromyces marxianus, Komagataella phaffii (also known as Pichia pastoris) have emerged as attracting cell factories to produce organic acids, natural products from plants, vaccines and antibodies. The development of yeast strains benefits not only their applications but also the discovery of novel mechanisms that provide a basis for studying other more complicated eukaryotic systems, including human being. In the past few years, great progress has been made in the characterization of novel yeast species or strains, metabolic engineering and genome editing of yeasts, as well as exploration of both wild yeasts and engineered yeast strains in various applications. In this special issue, we would like to present valuable latest findings in the development and utilization of yeast resources. Both dedicated review and research articles are welcome for the special issue. We welcome articles related but not limited to the following contents:

  • Yeast diversity and its potential in industrial applications;
  • Advanced technologies for the development of yeast strains;
  • Metabolic engineering of yeast strains for bioproduction;
  • Synthetic biology and artificial intelligence of yeast host.

We wish that this special issue contributes to summarizing the latest progress in the related fields, which would promote the utilization of yeast resources for efficient biological manufacturing.

Prof. Dr. Xin-Qing Zhao
Guest Editor

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Keywords

  • yeast diversity
  • metabolic engineering
  • gene editing
  • synthetic biology
  • cell factory
  • artificial intelligence
  • lignocellulosic
  • biomass
  • biofuels
  • biorefinery

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Published Papers (2 papers)

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Research

17 pages, 1456 KiB  
Article
Genetically Improved Yeast Strains with Lower Ethanol Yield for the Wine Industry Generated Through a Two-Round Breeding Program
by Eduardo I. Kessi-Pérez, Melissa Gómez, William Farías, Verónica García, María Angélica Ganga, Amparo Querol and Claudio Martínez
J. Fungi 2025, 11(2), 137; https://doi.org/10.3390/jof11020137 - 11 Feb 2025
Cited by 1 | Viewed by 940
Abstract
Saccharomyces cerevisiae is a species of industrial significance in the production of alcoholic beverages; it is the main species responsible for the fermentation of grape must. One of the main current problems in the wine industry is high alcohol levels caused by climate [...] Read more.
Saccharomyces cerevisiae is a species of industrial significance in the production of alcoholic beverages; it is the main species responsible for the fermentation of grape must. One of the main current problems in the wine industry is high alcohol levels caused by climate change. Pre- and post-fermentation strategies are used to reduce the alcohol content in wines; however, they are inefficient, affect organoleptic properties, face legal restrictions, and/or increase production costs, which has motivated efforts to obtain microbiological solutions. In the present work, we carried out a two-round breeding program to obtain improved yeast strains with lower ethanol yield. The trait under study showed high heritability (0.619), and we were able to lower the ethanol yield by 10.7% in just one generation. We finally obtained a population composed of 132 strains, of which 6 were used to produce wine from natural grape musts on a pilot scale, highlighting improved strains “C2-1B4” and “C7-1B7” as those that showed the best results (alcohol levels between 0.3 and 1.5% ABV less than expected). Further studies are required to understand the connection between initial sugar concentration and ethanol yield, as well as the genetic variants underlying this phenotype. Full article
(This article belongs to the Special Issue Development and Utilization of Yeast Resources, 2nd Edition)
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17 pages, 2740 KiB  
Article
Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains Kluyveromyces marxianus JMY140K and Metschnikowia reukaufii JMY075
by Ting-Ting Fan, Chao Chen, Du-Wen Zeng, Feng-Lou Wang, Zhao-Xian Xu, Ming-Jie Jin, Yue Zou, Jun Li and Xin-Qing Zhao
J. Fungi 2025, 11(1), 20; https://doi.org/10.3390/jof11010020 - 31 Dec 2024
Cited by 1 | Viewed by 893
Abstract
γ-Aminobutyric acid (GABA) is a valuable amino acid widely used in food, healthcare, and agriculture. GABA bioproduction by budding yeasts has been commonly reported, but related studies using non-conventional yeasts remain limited. In this study, two non-conventional natural yeast strains, namely, Kluyveromyces marxianus [...] Read more.
γ-Aminobutyric acid (GABA) is a valuable amino acid widely used in food, healthcare, and agriculture. GABA bioproduction by budding yeasts has been commonly reported, but related studies using non-conventional yeasts remain limited. In this study, two non-conventional natural yeast strains, namely, Kluyveromyces marxianus JMY140K and Metschnikowia reukaufii JMY075, were identified as promising GABA producers, and M. reukaufii JMY075 was discovered to be a GABA producer. Enhanced GABA production was observed in the two yeast strains under stress conditions, including high temperature and high ethanol and acetic acid levels. In particular, K. marxianus JMY140K showed 7.93 times higher GABA titers under thermal stress than that of the control. External stress conditions significantly influenced the GABA production of these two yeast strains. The culture filtrate of K. marxianus JMY140K also showed promising activities in human skin cells. In addition, K. marxianus JMY140K could also produce GABA using rice straw hydrolysate, which indicated that it has the potential to produce GABA using renewable biomass. Our studies provide insight for further enhancing the GABA production of natural yeasts and promoting its biotechnology applications. Full article
(This article belongs to the Special Issue Development and Utilization of Yeast Resources, 2nd Edition)
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