Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.2
3.0
Journals
Animals
Special Issues
Veterinary and Comparative Reproductive Pathology
share announcement

Veterinary and Comparative Reproductive Pathology

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 20433

Special Issue Editors

Dr. Gabriele Marino

E-Mail Website
Guest Editor
Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy
Interests: transmissible tumours; reproductive pathology; tumour-like lesions
Special Issues, Collections and Topics in MDPI journals
Dr. Alessandra Sfacteria

E-Mail Website
Guest Editor
Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy
Interests: mammary tumours; translational medicine; cytokines
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Reproductive pathology is an interdisciplinary topic of the reproductive sciences. It is devoted to the understanding of the etiopathogenesis, morphology, and management of gynaecological and andrological diseases. The reproductive pathology of males focuses on the study of accessory glands, penis, and testis. Reproductive pathology of females includes the study of the cervix, uterine tube, ovary, uterus, vulva, vagina, and mammary gland. Genital organs modify continuously under hormonal control; the evaluation of pathological changes can be very challenging and implies a deep knowledge of the morphological and physiological changes during the reproductive cycles, such as the transition from puberty to adult age, from anoestrus to oestrus, and the changes of the uterus and mammary gland during pregnancy and puerperium. All kinds of approaches are welcome. Clinicopathological studies include the diagnostic procedures and the study of the biological characteristics of the disease. Classical gross and microscopic studies are powerful in helping to understand and classify the lesion in a comparative way. Molecular and genetic studies are ancillary investigations on pathologies able to support clinicians and pathologists in classification, staging, and treatment.

We invite original research papers, review articles, as well as short communications concerning infectious, degenerative, and proliferative lesions of the genital tract of wild and domestic animals. Additional topics about neonatal pathologies are welcome.

Dr. Gabriele Marino
Dr. Alessandra Sfacteria
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • reproduction
  • pathology
  • dysfunction
  • tumour
  • diagnostics
  • histology
  • immunohistochemistry
  • genetics

Published Papers (7 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Other

9 pages, 1699 KiB  
Article
Immunohistochemical Expression of p62 in Feline Mammary Carcinoma and Non-Neoplastic Mammary Tissue
by Gian Enrico Magi, Francesca Mariotti, Lorenzo Pallotta, Alessandro Di Cerbo and Franco Maria Venanzi
Animals 2022, 12(15), 1964; https://doi.org/10.3390/ani12151964 - 02 Aug 2022
Cited by 1 | Viewed by 1520
Abstract
The p62 protein, also called sequestosome 1 (SQSTM1), is a ubiquitin-binding scaffold protein. In human oncology, although the interest in the function of this protein is recent, the knowledge is now numerous, but its role in tumorigenesis is not yet clear. This preliminary [...] Read more.
The p62 protein, also called sequestosome 1 (SQSTM1), is a ubiquitin-binding scaffold protein. In human oncology, although the interest in the function of this protein is recent, the knowledge is now numerous, but its role in tumorigenesis is not yet clear. This preliminary study aims to evaluate the immunohistochemical expression of p62 in 38 cases of feline mammary carcinoma with different grades of differentiation and in 12 non-neoplastic mammary gland tissues, to assess the expression level and a possible correlation with malignancy. The expression of p62 was statistically higher in carcinoma compared to non-neoplastic mammary glands: 28 feline mammary carcinomas (73.7%) had a high p62 expression score, three (7.9%) had a moderate expression, while seven cases (18.4%) had a low expression. The grade of the differentiation of the carcinoma was not correlated with the p62 expression. This study represents the first approach in feline oncology that correlates p62 expression in feline mammary carcinoma. Our results, although preliminary, are similar to the results of human breast cancer, therefore, also in the cat, p62 could be considered a possible oncotarget. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Figure 1

13 pages, 622 KiB  
Article
Oxytetracycline Persistence in Uterine Secretion after Intrauterine Administration in Cows with Metritis
by Rositsa Mileva, Manol Karadaev, Ivan Fasulkov, Nikolina Rusenova, Nasko Vasilev and Aneliya Milanova
Animals 2022, 12(15), 1922; https://doi.org/10.3390/ani12151922 - 28 Jul 2022
Cited by 1 | Viewed by 1572
Abstract
Puerperal metritis in cows is often treated with antibacterial drugs. The prudent use of antibiotics in farm animals requires state-of-the-art knowledge of their pharmacokinetics and data from sensitivity tests of pathogenic bacteria. Changes in oxytetracycline levels in the uterine secretion over time after [...] Read more.
Puerperal metritis in cows is often treated with antibacterial drugs. The prudent use of antibiotics in farm animals requires state-of-the-art knowledge of their pharmacokinetics and data from sensitivity tests of pathogenic bacteria. Changes in oxytetracycline levels in the uterine secretion over time after intrauterine administration in cows with metritis were evaluated in relation to the sensitivity of pathogenic bacterial isolates. Oxytetracycline levels in plasma, milk and uterine secretion were determined via LC–MS/MS analysis. Pathogenic bacteria were isolated and their sensitivity to oxytetracycline was determined. The concentrations of oxytetracycline in the uterine secretion were 433.79 (39.17–1668.76) µg·mL−1 six hours after the third application at a dose of 8 mg/kg and 84.33 (1.58–467.55) µg·mL−1 96 h after the last treatment. These levels were higher than the minimum inhibitory concentrations—namely, between 4 and 64 µg·mL−1—against pathogenic isolates Trueperella pyogenes, Streptococcus intermedius, Escherichia coli and Bacillus pumilis. Higher concentrations over time were measured in milk rather than in plasma, indicating the need to monitor the withdrawal time for milk. The intrauterine infusion of oxytetracycline for three consecutive days resulted in the successful treatment of metritis in terms alleviating inflammation and restoring the estrus cycle in cows. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Figure 1

12 pages, 3160 KiB  
Article
Immune Cells and Immunoglobulin Expression in the Mammary Gland Tumors of Dog
by Alessandra Sfacteria, Ettore Napoli, Claudia Rifici, Daria Commisso, Giada Giambrone, Giuseppe Mazzullo and Gabriele Marino
Animals 2021, 11(5), 1189; https://doi.org/10.3390/ani11051189 - 21 Apr 2021
Cited by 7 | Viewed by 2270
Abstract
Inflammatory cells have a role in tumor progression and have prognostic and therapeutic potential. The immunohistochemical expression for Mast Cell Tryptase, Macrophage Marker, CD79a, IgA, IgM and IgG on 43 cases of canine mammary gland lesions was analyzed. In hyperplasia, a few B [...] Read more.
Inflammatory cells have a role in tumor progression and have prognostic and therapeutic potential. The immunohistochemical expression for Mast Cell Tryptase, Macrophage Marker, CD79a, IgA, IgM and IgG on 43 cases of canine mammary gland lesions was analyzed. In hyperplasia, a few B cells (BCs) and Tumor-Associated Macrophages (TAMs) were observed, while the number of Tumor-Associated Mast Cells (TAMCs) was the highest. In the peritumoral stroma of malignant lesions, low number of TAMCs and a high number of TAMAs and BCs were present. Immune cells of each type were always lower in the intratumoral than peritumoral stroma. Positivity to CD79a was also detected in the epithelial cells of simple and micropapillay carcinomas. Immunoglobulin reactivity was mainly located in the epithelial cells where an intense positivity to IgA and IgG and a weak positivity for IgM were detectable. On the basis of our preliminary results and literature data, we suggest that such cells and molecules could be directly involved in the biology of canine mammary gland tumors. In breast cancer, stromal inflammatory cells and cancer derived immunoglobulins have been correlated with the progression, malignancy and poor prognosis of the tumor. The results herein reported show that the dog’s mammary gland epithelium also expresses immunoglobulins, and they mostly show a direct relationship with the infiltration of macrophages. In addition, this study shows that the infiltration of mast cells, B-cells and macrophages varies depending on the degree of malignancy of neoplasia. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Figure 1

9 pages, 546 KiB  
Article
Use of Spinal Anaesthesia with Anaesthetic Block of Intercostal Nerves Compared to a Continuous Infusion of Sufentanyl to Improve Analgesia in Cats Undergoing Unilateral Mastectomy
by Vincenzo Cicirelli, Pasquale Debidda, Nicola Maggio, Michele Caira, Daniela Mrenoshki, Giulio G. Aiudi and Giovanni M. Lacalandra
Animals 2021, 11(3), 887; https://doi.org/10.3390/ani11030887 - 20 Mar 2021
Cited by 12 | Viewed by 2695
Abstract
Unilateral mastectomy is a common surgical procedure in feline species and requires postoperative pain management. Our study aimed to evaluate the analgesic efficacy of subarachnoid anaesthesia combined with an intercostal nerve block, in comparison with the use of sufentanyl citrate administered as a [...] Read more.
Unilateral mastectomy is a common surgical procedure in feline species and requires postoperative pain management. Our study aimed to evaluate the analgesic efficacy of subarachnoid anaesthesia combined with an intercostal nerve block, in comparison with the use of sufentanyl citrate administered as a constant-rate infusion (CRI). Twenty cats were randomly divided into two groups (n = 10/group) based on the analgesic protocol used: the first received loco-regional anaesthesia with levobupivacaine (LR group), and the second received a CRI of sufentanyl (SUF group). The evaluation criteria during surgery were the need for a bolus of fentanyl in the event of an increased heart rate or increased blood pressure. In the postoperative period, the levels of comfort/discomfort and pain were used to obtain a score according to the UNESP-Botucatu multimodal scale. Subjects who scored above seven received analgesic drug supplementation. Intraoperative analgesia was satisfactory, with good haemodynamic stability in both groups. Four patients in the LR group required an extra dose of methadone after they achieved the sternal decubitus position, whereas those in the SUF group required many more doses. The analgesia achieved in the LR group was more satisfactory than that in the SUF group. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Figure 1

10 pages, 2871 KiB  
Article
Pseudo-Placentational Endometrial Hyperplasia in the Bitch: Case Series
by Gabriele Marino, Alessandra Sfacteria, Giuseppe Catone, Antonina Zanghì, Fabiana Pecchia, Angela Difrancesco and Marco Russo
Animals 2021, 11(3), 718; https://doi.org/10.3390/ani11030718 - 06 Mar 2021
Viewed by 5962
Abstract
Canine pseudo-placentational endometrial hyperplasia differs from the classical form of cystic endometrial hyperplasia for the well-organized tissue architecture resembling the canine placenta. After the discovery, it has been inconstantly reported. The present work reports the clinicopathological details of six spontaneous cases retrieved retrospectively [...] Read more.
Canine pseudo-placentational endometrial hyperplasia differs from the classical form of cystic endometrial hyperplasia for the well-organized tissue architecture resembling the canine placenta. After the discovery, it has been inconstantly reported. The present work reports the clinicopathological details of six spontaneous cases retrieved retrospectively from a large database. The lesion was found in young non-pregnant female dogs (median 2.0 years) at the end of dioestrus. It could be imaged by ultrasound and was always grossly detectable as single or multiple uterine enlargements of 2–3 cm in diameter with a villous whitish tissue growing on the mucosa and occluding the lumen. Histology confirmed the tissue architecture of the canine placenta with a basal glandular layer, a connective band, a spongy layer and a tortuous and compact labyrinth, often poorly recognizable. The pseudo-placentational hyperplasia is a non-inflammatory proliferative lesion although numerous mast cells inhabit the connective band, and a superimposed inflammatory infiltrate was seen in a case. Canine pseudo-placentational endometrial hyperplasia has very peculiar features, and it is a model for canine placentation and may help to better understand the cystic endometrial hyperplasia/pyometra complex. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Graphical abstract

13 pages, 6211 KiB  
Article
Contrast-Enhanced Ultrasound Imaging of Prostate Gland in Neutered Dogs
by Stefano Spada, Gary C. W. England, Massimo Vignoli, Augusto Carluccio and Marco Russo
Animals 2021, 11(2), 559; https://doi.org/10.3390/ani11020559 - 20 Feb 2021
Cited by 5 | Viewed by 2688
Abstract
Prostatic neoplasia (PN) occurs in 5–7% of dogs with prostatic disease, with castrated dogs having the same or higher prevalence when compared to intact dogs. Considering the promising results achieved by performing contrast-enhanced ultrasound (CEUS) in intact dogs to detect PN, the present [...] Read more.
Prostatic neoplasia (PN) occurs in 5–7% of dogs with prostatic disease, with castrated dogs having the same or higher prevalence when compared to intact dogs. Considering the promising results achieved by performing contrast-enhanced ultrasound (CEUS) in intact dogs to detect PN, the present study aimed to acquire data on the prostatic perfusion pattern in neutered dogs. CEUS was performed in 64 neutered dogs, using a 5–7.5 MHz linear transducer with coded harmonic capability, dedicated analytical software, and a second-generation contrast agent, SonoVue. After B-mode evaluation was performed to assess mean prostate volume, the CEUS examination was undertaken. The flow of contrast agent was visible 10 s after injection. The subcapsular vessels were highlighted and produced rapid peripheral rim enhancement. Subsequently, the contrast agent reached the prostatic urethra via the parenchymal arterioles and gradually reached the entire prostate. Perfusion peak intensity (PPI) and time to peak (TTP) values were respectively 45.3% and 34.1 s. The measured parameters were compared with those obtained in previous studies on intact dogs with normal and with pathological patterns. In this study, CEUS showed features that may be promising for its use as a diagnostic tool for early detection of PN in neutered dogs. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Figure 1

Other

Jump to: Research

14 pages, 67774 KiB  
Case Report
Ultrasound Examination of Unilateral Seminoma in a Salernitano Stallion
by Brunella Anna Giangaspero, Roberta Bucci, Francesca Del Signore, Massimo Vignoli, Jasmine Hattab, Gina Rosaria Quaglione, Lucio Petrizzi and Augusto Carluccio
Animals 2022, 12(7), 936; https://doi.org/10.3390/ani12070936 - 06 Apr 2022
Cited by 2 | Viewed by 2227
Abstract
An 18-year-old Salernitano stallion developed a progressive enlargement of the left testicle over eight months. An ultrasound evaluation was performed, along with a hormonal profile. A histopathological evaluation of the testis was performed after unilateral orchiectomy. On B-mode ultrasound examination, testicular parenchyma was [...] Read more.
An 18-year-old Salernitano stallion developed a progressive enlargement of the left testicle over eight months. An ultrasound evaluation was performed, along with a hormonal profile. A histopathological evaluation of the testis was performed after unilateral orchiectomy. On B-mode ultrasound examination, testicular parenchyma was characterized by the loss of internal structure, with the presence of multiple coalescing, nodular, well-defined and heterogeneous lesions with capsule deformity, appearing with an irregular profile. On dissection, the testicular parenchyma bulged over the cut section, confirming the increase in size. Microscopically, the lesion consisted mainly of large, densely packed, polygonal-to-round-shaped neoplastic cells. Mitotic figures were plentiful and frequently atypical; further microscopic features included apoptosis and necrosis. At immunohistochemistry, the entire neoplasm showed strong and diffuse immunolabeling for vimentin, while CD117-specific immunoreactivity was only observed in scattered clusters of neoplastic cells. Based on the gross, microscopic and IHC findings, a diagnosis of diffuse seminoma was made. Three months later, a follow-up examination showed no evidence of recurrence and the preservation of reproductive abilities. The case presented shows an unusual ultrasonographic pattern for seminoma and the basis of the correlation between the characteristics of the sonoelastographic examination and histological diagnosis. Full article
(This article belongs to the Special Issue Veterinary and Comparative Reproductive Pathology)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-7
Back to TopTop