In Vitro, In Vivo and Ex Vivo Veterinary Oncology Research and Treatment for Companion Animals

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: 10 September 2025 | Viewed by 477

Special Issue Editors


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Guest Editor
Department of Veterinary Sciences, University of Messina, Via G. Palatucci, 98168 Messina, Italy
Interests: veterinary pathology; mammary gland tumours; tumour microenvironment; translational medicine
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Guest Editor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d’Alcontres 31, 98166 Messina, Italy
Interests: veterinary surgery; veterinary anaesthesia; translational medicine
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Biotechnology Research and Diagnostics, Histopathology and Immunohistochemistry Laboratory, Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, Italy
Interests: veterinary pathology; oncology; mammary tumours; histopathology; immunohistochemistry

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Guest Editor
Department of Veterinary Sciences, University of Messina, Via G. Palatucci, 98168 Messina, Italy
Interests: veterinary oncology; mammary tumours; translational medicine; tumour microenvironment

Special Issue Information

Dear Colleagues,

Veterinary oncology has made huge progresses in recent years, but there are still many areas that need attention. One of the greatest challenges in oncology is to identify an experimental model that can reproduce the complexity and dynamism of the tumour in order to find targeted treatments. In vitro cell cultures are the starting point for providing meaningful insights into specific research questions. However, these do not fully replicate the tumour microenvironment. In vivo models overcome this obstacle but are more time-consuming and often pose ethical concerns. Ex vivo cultures represent three-dimensional models that maintain the complexity of the tumour biology, often including the stromal microenvironment, and represent a promising method that needs to be exploited in further studies.

A deep knowledge of tumour biology makes it possible to identify new molecular and cellular targets and recognise the best pharmacological or surgical treatment.

This Special Issue aims to collect papers that further investigate veterinary oncology using in vitro, in vivo, or ex vivo research methods to improve the knowledge of tumours and their possible treatment in companion animals.

Dr. Alessandra Sfacteria
Dr. Cecilia Vullo
Dr. Roberto Puleio
Dr. Giada Giambrone
Guest Editors

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Keywords

  • in vitro, in vivo, ex vivo methods
  • veterinary oncology
  • tumour microenvironment
  • tumour cell cultures
  • companion animals
  • cancer treatment

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Published Papers (1 paper)

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Research

20 pages, 5042 KiB  
Article
Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells
by Thayná Oliveira da Silva, Luís Gustavo Ramos de Moraes Calheiros, Felipe Barbosa, Fernanda Bueno Morrone, Liliana Rockenbach, Patrícia de Faria Lainetti, Antonio Fernando Leis Filho, Márcio de Carvalho, Carlos Eduardo Fonseca-Alves and Renée Laufer Amorim
Animals 2025, 15(11), 1589; https://doi.org/10.3390/ani15111589 - 29 May 2025
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Abstract
Bladder urothelial carcinoma (UC) is an aggressive malignancy in both humans and dogs, with limited treatment options. Owing to their biological and environmental similarities with humans, dogs serve as a valuable model for UC research. Standard treatments, including surgery, chemotherapy, and anti-inflammatory agents, [...] Read more.
Bladder urothelial carcinoma (UC) is an aggressive malignancy in both humans and dogs, with limited treatment options. Owing to their biological and environmental similarities with humans, dogs serve as a valuable model for UC research. Standard treatments, including surgery, chemotherapy, and anti-inflammatory agents, have shown limited efficacy. Curcumin, a bioactive compound derived from turmeric, has demonstrated anticancer properties, but its potential in canine UC remains poorly understood. In this study, we evaluated the effects of curcumin, D6 turmeric, and mitoxantrone hydrochloride on canine and human UC cell lines. Cell viability was assessed via the MTT assay, apoptosis via flow cytometry, and gene expression (β-catenin, β1-integrin, CDH1, MMP-2, MMP-9, and TIMP-2) via quantitative PCR. Migration capacity was analyzed using a Transwell assay. Curcumin and D6 turmeric reduced cell viability and migration, while mitoxantrone hydrochloride exhibited strong cytotoxicity, especially in canine cells. Curcumin also induced apoptosis and modulated genes involved in epithelial–mesenchymal transition and invasion. The interindividual differences in response suggest underlying genetic variability and highlight the need for personalized therapeutic approaches. These findings suggest that curcumin and D6 turmeric hold promise as complementary therapies for canine UC, justifying further in vivo investigations. Full article
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