Animals

Mineralization within the orbital ligament (OL) is occasionally observed on canine head computed tomography (CT) examinations, typically without associated clinical signs. This feature has been only briefly mentioned in the veterinary literature. The present retrospective descriptive study evaluated 402 dogs to determine the prevalence and CT characteristics of OL mineralization, including its location, morphology, margins, symmetry, size, and attenuation. Associations with signalment, medical history and concurrent mineralization were also assessed. Orbital ligament mineralization was identified in 157 of 402 dogs (39.1%). The lesion was consistently located dorsally (100%), and was most often symmetrical, triangular, well-defined and heterogenous. The presence of OL mineralization was significantly associated with increasing age and body weight, as well as with concurrent mineralization in other sites, such as lungs and ears. The lesion was significantly less frequent in brachycephalic dogs. No associations were found with facial trauma, orbital disease or other pathological conditions. Orbital ligament mineralization appears to be a common incidental finding in canine head CT studies, most likely representing a benign, age-related, and non-pathological change.

Since cats often struggle to acclimate to laboratory settings, a key requirement for effective testing, we adapted Ainsworth’s Strange Situation Test (SST) for use in environments familiar to the cats. We examined 82 cat–caregiver dyads from private households and animal shelters using a standardised five-phase video procedure comprising distinct phases of presence, separation, and reunion between the cats and their human caregivers. Seven attachment-related behaviours were recorded via focal animal sampling and analysed using mixed models. Significant differences were observed in exploratory behaviour, physical contact, play (social, object, and locomotor), passive behaviour, and vocalisation. Cats from private homes exhibited more exploratory and play behaviour, particularly after reuniting with their caregiver, indicating a positive influence of the familiar person’s presence. Cats from animal shelters exhibited reduced play behaviour, more passive behaviour, and more frequent purring, particularly following reunion. Physical contact increased following separations, indicating attachment. Meowing was most frequent during absences and reunions, possibly reflecting stress and renewed social contact. Outdoor access emerged as a potential influencing factor for exploratory behaviour. In summary, attachment behaviours in cats were significantly influenced by the living environment, access to the outdoors, and the presence of familiar or unfamiliar individuals. Certain test phases elicited particularly strong behavioural responses.

Precise intraoperative identification of the canine thoracic duct remains challenging due to anatomical variability and limited visualization. This exploratory cadaveric feasibility study aimed to describe the technical applicability of fluorescence-guided thoracic duct mapping using video-assisted thoracoscopy (VATS) and robot-assisted thoracoscopy (Versius™ system). Four adult Beagle cadavers underwent bilateral thoracoscopic exploration after intranodal injection of indocyanine green (ICG, Verdye^®, 0.05 mg/kg; 0.5 mL). Near-infrared (NIR) fluorescence imaging enabled real-time visualization of the thoracic duct and its branches. Fluorescence quality was quantitatively characterized using signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and contrast resolution (CR) calculated from standardized image frames. Both approaches achieved successful duct identification in all cadavers. VATS provided brighter overall fluorescence, whereas the robotic-assisted approach offered stable imaging, enhanced instrument dexterity, and improved duct-to-background discrimination. These findings confirm the feasibility of fluorescence-guided thoracic duct identification using both minimally invasive modalities in canine cadavers. The standardized assessment of optical parameters proposed here may support future in vivo studies to optimize imaging protocols and evaluate the clinical impact of fluorescence-guided thoracic duct surgery in dogs.