The Role of Reproductive Microbiota in Animal Fertility and Reproductive Health

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: 1 December 2025 | Viewed by 567

Special Issue Editors


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Guest Editor
Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), 75007 Uppsala, Sweden
Interests: veterinary medicine; reproduction; reproductive biotechnology; bacteriology; antimicrobial resistance

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Guest Editor
AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
Interests: male reproduction; sperm; andrology; antibiotics; bioactive compounds

Special Issue Information

Dear Colleagues,

Recent advances in microbiome research have highlighted the critical role of reproductive microbiota in animal fertility, reproductive health, and breeding programs. The reproductive microbiota, comprising the diverse microbial communities inhabiting the reproductive system, can significantly influence the quality of sperm, the development of embryos, and the overall success of reproduction. Understanding the interactions between host organisms and their microbiota provides insights that could enhance the success of reproduction, the prevention of diseases, and animal conservation efforts.

In both male and female reproductive systems, the balance of microbial populations plays an important role in maintaining reproductive health. Moreover, the microbiota of other organ systems, such as the gastrointestinal tract and mammary glands, may indirectly influence reproductive health via complex microbiome–host interactions. The incorporation of microbiota-based strategies into assisted reproductive technologies and conservation programs offers opportunities regarding the enhancement of reproductive outcomes in both domestic and wild animal populations.

By addressing these key issues, this Special Issue aims to provide a clearer understanding of how the reproductive microbiota influence fertility, reproductive health, and breeding practices. It is our hope that this Special Issue will stimulate further research and contribute to the development of innovative microbiota-based interventions that improve reproductive outcomes in both domestic and wild animals.

Dr. Aleksandar Cojkic
Dr. Michal Ďuračka
Guest Editors

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Keywords

  • reproductive microbiota
  • animal fertility
  • male reproductive health
  • female reproductive tract
  • mammary gland microbiota
  • microbiome-host interactions
  • assisted reproductive technologies
  • animal conservation

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Published Papers (1 paper)

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Research

22 pages, 1855 KiB  
Article
Taxonomic Profile of Cultivable Microbiota from Adult Sheep Follicular Fluid and Its Effects on In Vitro Development of Prepubertal Lamb Oocytes
by Slavcho Mrenoshki, Letizia Temerario, Antonella Mastrorocco, Grazia Visci, Elisabetta Notario, Marinella Marzano, Nicola Antonio Martino, Daniela Mrenoshki, Giovanni Michele Lacalandra, Graziano Pesole and Maria Elena Dell’Aquila
Animals 2025, 15(13), 1951; https://doi.org/10.3390/ani15131951 - 2 Jul 2025
Viewed by 372
Abstract
The aims of the present study were to analyze the taxonomic profile and to evaluate the functional effects of sheep FF cultivable microbiota on prepubertal lamb oocytes PLOs developmental potential. Ovarian FFs were recovered from slaughtered adult sheep via the aspiration of developing [...] Read more.
The aims of the present study were to analyze the taxonomic profile and to evaluate the functional effects of sheep FF cultivable microbiota on prepubertal lamb oocytes PLOs developmental potential. Ovarian FFs were recovered from slaughtered adult sheep via the aspiration of developing follicles and used for microbiota propagation. Bacterial pellets underwent 16S rRNA gene sequencing and targeted culturomics, whereas cell-free supernatants were used as supplements for the in vitro maturation (IVM) of slaughtered PLOs. For the first time, bacteria presence in adult sheep FF was detected, with the first report of Streptococcus infantarius subsp. infantarius (as a species) and Burkholderia cepacia (as a genus and species) in either animal or human FF. The short- and long-term effects of bacterial metabolites on PLO maturation and embryonic development were demonstrated. As short-term effects, the addition of FF microbiota metabolites did not affect the oocyte nuclear maturation and mitochondria distribution pattern, except in one of the examined supernatants, which reduced all quantitative bioenergetic/oxidative parameters. As long-term effects, one of them reduced the total cleavage rate after in vitro embryo culture (IVC). In conclusion, microbiota/bacteria are present in adult sheep FF and may influence reproductive outcomes in vitro. Future studies may reveal the beneficial in vitro effects using the microbiome from preovulatory follicles. Full article
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