Recent Advances in Cephalopod Biology Research

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Aquatic Animals".

Deadline for manuscript submissions: 31 March 2026 | Viewed by 6665

Special Issue Editors


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Guest Editor
1. Department of Life and Environmental Science, University of Cagliari, 09126 Cagliari, Italy
2. Stazione Zoologica Anton Dohrn, CRIMAC, Calabria Marine Centre, Department of Integrative Marine Ecology (EMI), C.da Torre Spaccata, 87071 Amendolara, CS, Italy
Interests: cephalopods; fishery management; mark recapture; reproductive strategies; sexual maturity; age; growth

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Guest Editor
Department of Biology, University of Naples Federico II, Via Cinthia, 80126 Naples, Italy
Interests: marine biodiversity; cephalopods; neuroetology; animal behavior; chemical communication; adult neurogenesis

Special Issue Information

Dear Colleagues,

Cephalopods are a very ancient and highly evolved group with complex and fascinating behaviour and a great capacity for learning and memory due to the development of the brain and specialized sensory organs. Thanks to their short lifespan (less than one or two years), they have a special ecology and are unique important component of marine ecosystems globally, playing a key role in the transfer of biomass from low trophic positions to higher predators, in coastal, oceanic and deep-sea ecosystems. Moreover, several of them are important resources of high commercial relevance on a global scale, but unfortunately, anthropogenic stressors, such as fishing, pollution, intensive activities in coastal areas, invasive species, climate change, etc., are altering the structure and functioning of ecosystems, leading to the degradation of their habitats.

With this in mind, this Special Issue aims to explore all new advances in Cephalopod research through original research articles or specific reviews. Research areas may include (but are not limited to) the following: anatomy, reproduction strategy, age and growth, fishery and management research, aquaculture, ethology, welfare, tools to improve animal welfare in research.

Dr. Blondine Agus
Prof. Anna Di Cosmo
Guest Editors

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Keywords

  • cephalopods
  • fishery research
  • aquaculture
  • welfare
  • anatomy
  • age
  • reproduction
  • behavior

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Published Papers (2 papers)

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Research

26 pages, 2960 KB  
Article
Tissue-Specific Gene Expression of Digestive Tract Glands in Paroctopus digueti: Insights for Cephalopod Biology and Aquaculture
by María G. Martínez-Morales, Oscar E. Juárez, Dariel Tovar-Ramírez, Clara E. Galindo-Sánchez, Claudia Ventura-López, Carlos Rosas, Héctor Nolasco-Soria and Bertha Patricia Ceballos-Vázquez
Animals 2025, 15(21), 3224; https://doi.org/10.3390/ani15213224 - 6 Nov 2025
Viewed by 894
Abstract
Pacific pygmy octopus Paroctopus digueti is a promising model for cephalopod research and aquaculture; its feeding and nutritional biology remain poorly understood. The anterior salivary glands (ASG), posterior salivary glands (PSG), and digestive gland (DG) are central to these processes, but molecular comparisons [...] Read more.
Pacific pygmy octopus Paroctopus digueti is a promising model for cephalopod research and aquaculture; its feeding and nutritional biology remain poorly understood. The anterior salivary glands (ASG), posterior salivary glands (PSG), and digestive gland (DG) are central to these processes, but molecular comparisons are lacking. To address this gap, we performed a transcriptomic study to explore the enzymatic repertoire and functional specialization of these tissues. Total RNA was extracted from ASG, PSG, and DG of three pre-adult individuals collected in La Paz Bay, Mexico. RNA-Seq libraries were sequenced, and a non-redundant multi-tissue transcriptome was assembled. The ASG displayed high expression of neuropeptides, playing a role in neuroendocrine regulation. The PSG showed elevated protease expression, supporting its function in extracellular digestion, alongside toxins that reinforce its role as a venom gland. The DG was enriched in proteins linked to biomolecule catabolism and antimicrobial peptides, alluding to metabolic specialization and immune defense. These results were validated by qPCR, and target genes were also amplified in Octopus maya and O. hubbsorum, showing some similarities in expression patterns. Overall, our findings suggest strong glandular specialization in P. digueti, providing insights into cephalopod digestive physiology and supporting its value as a model species. Full article
(This article belongs to the Special Issue Recent Advances in Cephalopod Biology Research)
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15 pages, 5151 KB  
Article
The Persistence of Memory: Behavioral Analysis and Arm Usage of a Nine-Armed Octopus vulgaris
by Sam Ellington Soule, Miguel Cabanellas-Reboredo, Ángel F. González, Hidde Juijn and Jorge Hernández-Urcera
Animals 2025, 15(7), 1034; https://doi.org/10.3390/ani15071034 - 3 Apr 2025
Viewed by 4670
Abstract
Anatomical abnormalities in octopuses, whose behavior is facilitated by flexible, neuron-rich arms, offer insights into life histories and the neurological implications of understudied conditions such as bifurcation. Although documentation is scarce, here we present in situ videos of nine-armed O. vulgaris with a [...] Read more.
Anatomical abnormalities in octopuses, whose behavior is facilitated by flexible, neuron-rich arms, offer insights into life histories and the neurological implications of understudied conditions such as bifurcation. Although documentation is scarce, here we present in situ videos of nine-armed O. vulgaris with a functional bifurcated R1 arm. Analysis using RDAs and GLMs investigated the impact of the bifurcated arm on behavior and examined changes during growth. Analysis revealed a differential usage of between the bifurcated arms in addition to an initial specialization of the bifurcated arms for actions below the body, decreasing over time for only one of the arms as grew. Further, bifurcated and regrown arms were utilized more in safe behaviors than risky ones, with more severely injured arms showing a higher frequency of use in safe behaviors. These findings contribute to the growing knowledge of arm usage in octopuses, suggesting that arm bifurcation may lead to branchial neural differentiation and potentially indicate post-traumatic associated in O. vulgaris. Full article
(This article belongs to the Special Issue Recent Advances in Cephalopod Biology Research)
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