Agronomic Strategies for Managing Insecticide Resistance in Crop Pests

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Pest and Disease Management".

Deadline for manuscript submissions: 20 May 2026 | Viewed by 866

Special Issue Editors


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Guest Editor
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Interests: insecticide resistance evolution; cytochrome P450; gene regulation; rice plant hoppers; pest management
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E-Mail Website
Guest Editor
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: insecticide resistance; whitefly; gene regulation; pest control; cytochrome P450; insect signaling pathway
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues, 

Insecticide resistance in crop pests poses a significant threat to global food security and sustainable agriculture. Understanding the molecular mechanisms driving resistance, such as mutations in target-site genes, the overexpression of detoxification enzymes including cytochrome P450s, and alterations in insect signaling pathways, is critical for developing effective management strategies. Recent advances in genomics, transcriptomics, and proteomics have provided powerful tools to elucidate these mechanisms at unprecedented resolution.

This Special Issue, titled “Agronomic Strategies for Managing Insecticide Resistance in Crop Pests”, will bring together cutting-edge research and comprehensive reviews that bridge molecular biology with practical agronomy. Particular emphasis will be placed on agronomic strategies, including crop rotation, refuge design, host plant resistance, optimized pesticide application schemes, integration of biological control, and landscape-level practices. At the same time, studies on the genetic and biochemical bases of resistance, novel molecular targets, and resistance monitoring methods are also welcome, especially when they inform or support these agronomic approaches.

By integrating molecular insights with field-level applications, this Special Issue will advance science-based, sustainable solutions for managing insecticide resistance and safeguarding crop productivity in the face of current and future agricultural challenges.

Dr. Youhui Gong
Prof. Dr. Xin Yang
Guest Editors

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Keywords

  • insecticide resistance
  • cytochrome P450
  • gene regulation
  • target-site mutation
  • insect signaling pathway
  • rice planthopper
  • whitefly
  • pest management
  • integrated pest management
  • sustainable agriculture

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Published Papers (1 paper)

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Research

17 pages, 6748 KB  
Article
Referenced Transcriptomics Identifies a Core Set of Cytochrome P450 Genes Driving Broad-Spectrum Insecticide Detoxification in Phthonandria atrilineata
by Delong Guan, Jing Song, Yue Qin, Lei Xin, Xiaodong Li and Shihao Zhang
Agronomy 2025, 15(11), 2561; https://doi.org/10.3390/agronomy15112561 - 5 Nov 2025
Viewed by 499
Abstract
Phthonandria atrilineata, also known as the mulberry looper, is a major defoliator of mulberry trees. This feeding behavior directly affects the growth of the trees and reduces the quality and yield of mulberry leaves for its use in sericulture. Despite its importance [...] Read more.
Phthonandria atrilineata, also known as the mulberry looper, is a major defoliator of mulberry trees. This feeding behavior directly affects the growth of the trees and reduces the quality and yield of mulberry leaves for its use in sericulture. Despite its importance the molecular basis of its resistance to insecticides remains poorly understood. Therefore, this study aimed to comprehensively characterize the cytochrome P450 monooxygenases (P450s) gene family in P. atrilineata and identify key effectors responsible for responses to diverse chemical stressors. We integrated genome-wide re-annotation, phylogenetic analysis, and comparative transcriptomics following exposure to five chemically distinct insecticides. We identified a high-confidence set of 70 P450 genes, dominated by the CYP6 and CYP4 families, whose expansion was driven by tandem gene duplication. Transcriptomic analysis revealed a powerful yet highly selective “elite-driven” response, wherein a small subset of P450s was strongly induced by multiple insecticides. Random Forest and Support Vector Machine (SVM) models converged with differential expression data to pinpoint a core trio of P450s as primary drivers of detoxification: two generalists, CYP6(09521) and CYP6(04876), responsive to all compounds, and one potent specialist, CYP4(04803), exhibiting massive induction to a specific subset of insecticides. Our findings uncover a complex, energy-efficient metabolic strategy in P. atrilineata and identify pivotal P450 genes for broad-spectrum detoxification. These genes represent high-priority targets for developing molecular diagnostic tools for resistance monitoring and informing scientifically guided insecticide rotation strategies. Full article
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