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Agronomy
Special Issues
Plant Tissue Culture and Regeneration Techniques for Crop Enhancement
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Plant Tissue Culture and Regeneration Techniques for Crop Enhancement

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1277

Special Issue Editors

Dr. Thiresia-Teresa Tzatzani

E-Mail Website
Guest Editor
Hellenic Agricultural Organization (ELGO-DIMITRA), Chania, Greece
Interests: subtropical plant physiology; plant propagation techniques; tissue culture
Dr. Georgios Tsoktouridis

E-Mail Website
Guest Editor
Hellenic Agricultural Organization (ELGO-DIMITRA), Thermi, Thessaloniki, Greece
Interests: floriculture; plant conservation; phylogenetics; genomics

Special Issue Information

Dear Colleagues,

This Special Issue entitled “Plant Tissue Culture and Regeneration Techniques for Crop Enhancement” aims to highlight the pivotal role of tissue culture and regeneration methods in modern plant biotechnology and crop improvement. Contemporary agriculture requires effective strategies for plant propagation, and tissue culture provides a reliable tool to support scientific research and applications in multiple fields: genetic improvement, genomics and synthetic biology, the enhancement of genetic diversity, physiology studies, the production of secondary metabolites, the conservation of rare and endangered plant species, large-scale propagation, increased crop productivity, and the mitigation of the global food security challenge.

The advantages of tissue culture include its precision, rapid implementation, contribution to public health safety, and resource efficiency, supporting both the scientific community and end users. Furthermore, plant tissue culture techniques are being integrated into strategies for biodiversity conservation, the development of resistant varieties against biotic and abiotic stress factors, and the advancement of sustainable agricultural practices.

This Special Issue invites researchers to submit original research articles, reviews, and case studies that showcase new scientific achievements in this field. The ultimate goal is to promote the exchange of knowledge, strengthen collaborations, and contribute to sustainable agriculture that meets the growing demands of society.

Dr. Thiresia-Teresa Tzatzani
Dr. Georgios Tsoktouridis
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant tissue culture
  • regeneration techniques
  • crop improvement
  • genetic diversity
  • in vitro propagation
  • somatic embryogenesis
  • stress tolerance
  • sustainable agriculture

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Published Papers (1 paper)

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Research

28 pages, 3642 KB  
Article
In Vitro Phytochemical Profiling, and Antioxidant Activity Analysis of Callus and Cell Suspension Cultures of Washingtonia filifera Elicited with Chitosan
by Huda Enaya Mahood, Virginia Sarropoulou, Thalia Tsapraili and Thiresia-Teresa Tzatzani
Agronomy 2026, 16(1), 106; https://doi.org/10.3390/agronomy16010106 - 31 Dec 2025
Viewed by 901
Abstract
Washingtonia filifera is important for its ecological, economic, cultural, horticultural, ornamental, and medicinal potential. Elicitation of in vitro cultures presents a promising and efficient method for the large-scale production of valuable bioactive compounds. This study assessed the effect of chitosan concentration (0, 20, [...] Read more.
Washingtonia filifera is important for its ecological, economic, cultural, horticultural, ornamental, and medicinal potential. Elicitation of in vitro cultures presents a promising and efficient method for the large-scale production of valuable bioactive compounds. This study assessed the effect of chitosan concentration (0, 20, 40, 60, 80, 100 mg L−1) on biomass growth [fresh weight (FW), dry weight (DW)] and phytochemical profile [total phenolic content (TPC), total flavonoid content (TFC), DPPH antioxidant activity, total phenolic productivity (TPP), total flavonoid productivity (TFP)] in W. filifera callus and cell suspension cultures. Among different plant growth regulator combinations tested, 3 mg L−1 2,4-D + 0.5 mg L−1 2ip gave higher callus induction (90%) (MS medium, 12 weeks). A maximum growth curve (FW: 180 mg) of cell suspension culture was achieved 7 weeks after initiation (shaker at 90 rpm for 24 h). Cell suspension exhibited higher FW, DW, TPC, TFC, DPPH, TPP, and TFP than callus, while flavonoid production was higher than phenolic production. FW and DW were higher in both systems, with 40 mg L−1 chitosan. Chitosan at 60 mg L−1 best enhanced the phytochemical profile of both the 4-week solidified callus and the 7-week liquid cell suspension (TPC: 29.9 and 32.1 mg GAE g−1 DW; TFC: 40.5 and 56.1 mg QE g−1 DW; TPP: 969.2 and 1122.6 mg L−1; TFP: 1313.9 and 1521.7 mg L−1; DPPH: 87.4 and 92.3%), respectively, while 40 mg L−1 chitosan was equally effective regarding DW, TFC, and TFP in cell suspension. Chitosan elicitation provides a powerful strategy to upregulate phenolic and flavonoid biosynthesis in W. filifera in vitro systems, conferring superior antioxidant potential. The identification of peak elicitation parameters (chitosan concentration, exposure time) allows for the targeted enhancement of bioactive compound yields, suggesting a viable path for industrial bioproduction and commercialization in pharmaceuticals, nutraceuticals, and functional foods, leveraging bioreactor technology for efficient scale-up. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Regeneration Techniques for Crop Enhancement)
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