Resistance-Related Gene Mining and Genetic Improvement in Crops—2nd Edition

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: 25 July 2026 | Viewed by 1522

Special Issue Editors


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Guest Editor
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China
Interests: potato; drought; ABA; cadmium stress
Special Issues, Collections and Topics in MDPI journals
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China
Interests: germplasm conservation; genetics and molecular biology; gene function; gene family; alfalfa; maize
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Research on crop stress resistance is a crucial topic in the field of agriculture. With climate change and environmental degradation, crops are facing increasingly severe non-biological stressors such as drought and salinity. Scientists are exploring drought-resistant genes through genetic improvement to enhance crop adaptability and yield stability. In recent years, utilizing molecular biology and genomics technologies has led to the successful discovery of multiple key drought-resistant genes, which have been introduced into crops through transgenic or hybrid breeding methods, achieving some breakthroughs. However, challenges remain, including insufficient depth in gene exploration, incomplete understanding of gene functions, and concerns about the safety of transgenic crops, thus impeding the progress of stress-resistance research. Future efforts should focus on strengthening fundamental research, delving deeper into drought-resistance mechanisms, exploring new genetic improvement approaches, and prioritizing ecological risk assessments to propel greater advancements in crop stress-resistance research.

In this Special Issue, we are soliciting research articles on novel and underexplored crop stress-resistance-related genes, as well as comprehensive reviews offering unique insights into resistance against non-biological stressors in major crops.

Dr. Panfeng Yao
Dr. Chen Lin
Guest Editors

Manuscript Submission Information

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Keywords

  • agriculture
  • plant science
  • staple crops
  • abiotic stress
  • plant breeding
  • genetic improvement

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Related Special Issue

Published Papers (2 papers)

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Research

16 pages, 2594 KB  
Article
Microtubule Dynamics Modulate Cold-Responsive Gene Expression in Brassica rapa
by Xinyi Zhang, Xiaoyun Dong, Guoqiang Zheng, Qian Luo, Zefeng Wu, Jinxiong Wang, Junmei Cui, Yan Fang, Zigang Liu and Jiaping Wei
Agronomy 2026, 16(7), 698; https://doi.org/10.3390/agronomy16070698 - 26 Mar 2026
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Abstract
Winter rapeseed (Brassica rapa L.) is an important crop for vegetable oil production in China. However, its productivity is frequently threatened by severe cold waves during winter. To investigate the role of the microtubule cytoskeleton in cold adaptation of winter rapeseed, a [...] Read more.
Winter rapeseed (Brassica rapa L.) is an important crop for vegetable oil production in China. However, its productivity is frequently threatened by severe cold waves during winter. To investigate the role of the microtubule cytoskeleton in cold adaptation of winter rapeseed, a microtubule stabilizer paclitaxel (Tax) and a microtubule depolymerizer colchicine (Col) were sprayed on winter rapeseed and transgenic proBrAFP1 Arabidopsis, respectively. The mRNA levels of cold-induced genes, along with cell membrane stability, antioxidant enzyme activities, and hormone levels were assessed under cold stresses of 4 °C and −4 °C. The results showed that low temperature significantly activated the proBrAFP1 promoter activity and increased the mRNA levels of core cold signaling pathway genes, such as C-REPEAT BINDING FACTORS (CBFs), Cyclic Nucleotide-Gated Channel (CNGC), OPEN STOMATA 1 (OST1) and Inducer of CBF EXPRESSION 1 (ICE1). Notably, under low-temperature stress, exogenous application of the microtubule stabilizer Tax markedly suppressed proBrAFP1-driven reporter activity in transgenic Arabidopsis, with consistent inhibition observed across both stem and leaf tissues; meanwhile, the Tax application alleviated reactive oxygen species (ROS) accumulation and mitigated membrane damage. In contrast, under the same low-temperature stress, the Col treatment exacerbated oxidative stress, enhanced lipid peroxidation, and elevated membrane damage. Collectively, these findings establish that microtubule regulators play indispensable roles in the cold stress response of winter rapeseed. It provides new insights into the mechanism by which plant microtubule cytoskeleton regulators mediate the cold response. Full article
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20 pages, 7805 KB  
Article
Heterologous Expression of Potato StCML19 Enhances Drought Tolerance in Transgenic Arabidopsis
by Jia Wei, Xinglong Su, Junmei Cui, Xianglin Sun, Jinjuan Ma, Zhenzhen Bi, Yuhui Liu, Zhen Liu, Yongwei Zhao, Yajie Li, Feng Zhao, Jiangping Bai, Panfeng Yao and Chao Sun
Agronomy 2026, 16(6), 674; https://doi.org/10.3390/agronomy16060674 - 23 Mar 2026
Viewed by 486
Abstract
Calmodulin-like proteins (CMLs) serve as core components in plant calcium signal transduction pathways, and they extensively modulate plant growth, development, and adaptive responses to various abiotic stresses. In this study, we cloned the StCML19 gene from potato and generated stable transgenic Arabidopsis thaliana [...] Read more.
Calmodulin-like proteins (CMLs) serve as core components in plant calcium signal transduction pathways, and they extensively modulate plant growth, development, and adaptive responses to various abiotic stresses. In this study, we cloned the StCML19 gene from potato and generated stable transgenic Arabidopsis thaliana lines constitutively expressing this gene to investigate its functional role under drought stress. Transcriptome analysis revealed that StCML19 was up-regulated under drought conditions. Phenotypic assays showed that overexpressing StCML19 notably increased the seed germination rate and root length of transgenic Arabidopsis under mannitol-induced osmotic stress, and greatly improved the plant survival rate under severe soil drought stress. Physiological analysis showed that when put under drought stress, transgenic plants had higher proline content, better SOD, CAT, and POD activities, and significantly less malondialdehyde (MDA) accumulation than wild-type plants. In addition, overexpression of StCML19 led to greater plant sensitivity to exogenous ABA, with inhibited root growth and delayed seed germination as indicators. Conclusively, this study is the first to make sense of the biological function of potato StCML19 in the drought stress response and views StCML19 as a promising candidate gene for the genetic improvement of drought-tolerant potato varieties. Full article
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