From Phenotype to Gene: A Holistic Approach to Improving Crop Agronomic Traits

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 822

Special Issue Editors


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Guest Editor
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: molecular breeding; disease-resistant breeding in maize

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Guest Editor
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: genomic-enhanced breeding; breeding tool development
Special Issues, Collections and Topics in MDPI journals

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Guest Editor Assistant
Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Interests: multidimensional plant phenotyping based on deep learning

Special Issue Information

Dear Colleagues,

Most agronomically important crop traits are quantitative in nature. These traits exhibit continuous variation due to polygenic control and complex genetic architectures, making their dissection at the population level particularly challenging. Recent breakthroughs in high-throughput phenomics, third-generation sequencing, and AI-driven analytics are revolutionizing our ability to study these traits. These technologies enable more precise quantitative trait dissection and predictive modeling, paving the way for data-driven molecular breeding strategies. As these tools continue to evolve, they will play an increasingly pivotal role in accelerating crop design breeding programs.

This Special Issue seeks to showcase cutting-edge strategies for high-throughput phenotyping, as well as the genetic dissection and genomic prediction of crop quantitative traits, with an emphasis on novel methodologies and their applications. In particular, we welcome papers on how high-throughput and time-series phenotyping technologies can improve trait measurement and how these data can be synergized with genomic approaches to identify the key loci governing quantitative traits. Ultimately, this knowledge can be translated into molecular design breeding strategies for trait enhancement.

We invite submissions in the form of original research, reviews, perspectives, and opinion articles on topics including, but not limited to, the following:

  1. Advanced phenotyping techniques for crop quantitative traits, including agronomic performance, stress resilience, and disease resistance.
  2. Genetic analysis and QTL mapping, such as combining ability analysis, QTL mapping, GWAS, and multi-omics integration.
  3. Breeding methodologies for trait improvement, including marker-assisted selection (MAS), genomic selection (GS), genomic–enviromic selection (GES), and gene editing applications.

By combining the fields of phenomics, genetics, and breeding, this Special Issue aims to advance the understanding and manipulation of complex traits for sustainable crop improvement.

Dr. Zifeng Guo
Dr. Hongwei Zhang
Guest Editors

Dr. Yinglun Li
Guest Editor Assistant

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • phenomics
  • quantitative trait locus (QTL)
  • genomic selection (GS)
  • genomic-enviromic selection (GES)
  • molecular design breeding

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Published Papers (1 paper)

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Research

16 pages, 2163 KiB  
Article
Arbuscular Mycorrhizal Fungi Mitigate Lead Toxicity in Maize by Restructuring Rhizosphere Microbiome and Enhancing Antioxidant Defense Mechanisms
by Xiaoxiang Zhang, Bin Zhao, Yan Zheng, Min Li, Huaisheng Zhang, Pingxi Wang, Shilin Chen, Xining Jin and Xiangyuan Wu
Agronomy 2025, 15(6), 1310; https://doi.org/10.3390/agronomy15061310 - 27 May 2025
Cited by 2 | Viewed by 432
Abstract
The remediation of lead (Pb)-contaminated soils through eco-friendly strategies is critical for sustainable agriculture. This study investigated the role of arbuscular mycorrhizal fungi (AMF) in enhancing maize tolerance to Pb stress and modulating rhizosphere microbial communities. A pot experiment was conducted with maize [...] Read more.
The remediation of lead (Pb)-contaminated soils through eco-friendly strategies is critical for sustainable agriculture. This study investigated the role of arbuscular mycorrhizal fungi (AMF) in enhancing maize tolerance to Pb stress and modulating rhizosphere microbial communities. A pot experiment was conducted with maize (Baiyu833) under four Pb concentrations (0, 900, 1800, 2700 mg·kg−1) and three AMF treatments: non-inoculation (Non), Funneliformis mosseae (Fm), or Rhizophagus intraradices (Ri). The results demonstrated that AMF inoculation significantly increased plant biomass, boosted antioxidant enzyme activities (SOD, POD), and reduced malondialdehyde (MDA) levels, mitigating Pb-induced oxidative stress. AMF restricted Pb translocation to aerial parts, with root Pb accumulation reaching 2110.76 mg·kg−1 (Fm) and 2090.56 mg·kg−1 (Ri) under Pb2700, enhancing phytostabilization. High-throughput sequencing revealed that AMF inoculation enriched α-diversity indices and restructured bacterial communities, favoring beneficial taxa like Promicromonospora, which are linked to heavy metal resistance and plant growth promotion. Principal coordinate analysis highlighted distinct clustering of microbial communities driven by AMF, emphasizing their role in alleviating Pb toxicity. These findings underscore that AMF enhance maize resilience to Pb by regulating antioxidant defense, immobilizing Pb in roots, and recruiting stress-tolerant rhizosphere microbiomes. This study provides insights into AMF-assisted phytoremediation as a sustainable strategy for Pb-contaminated soils. Full article
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