‘The Plastic Nile’: First Evidence of Microplastic Contamination in Fish from the Nile River (Cairo, Egypt)

Journal Description

Toxics — Open Access Journal

Toxics (ISSN 2305-6304; CODEN: TOXIC8) is an international peer-reviewed open access journal on all aspects of the toxicity of chemicals and materials, published quarterly online by MDPI.

Open Access - free for readers, with article processing charges (APC) paid by authors or their institutions.
High Visibility: Covered in Science Citation Index Expanded (SCIE) in the Web of Science, in Scopus and in Chemical Abstracts / SciFinder. Citations available in PubMed, full-text archived in PubMed Central from Vol. 3.
CiteScore (Scopus data): 3.7, which ranks 57/128 (Q2) in "Health, Toxicology and Mutagenesis" and 64/116 (Q3) in "Toxicology".
Rapid Publication: manuscripts are peer-reviewed and a first decision provided to authors approximately 15.6 days after submission; acceptance to publication is undertaken in 3.8 days (median values for papers published in this journal in the first half of 2020).
Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.

Impact Factor: 3.271 (2019)

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Latest Articles

Open AccessCase Report

Recycling Waste Electrical and Electronic Equipment (WEEE) and the Management of Its Toxic Substances in Taiwan—A Case Study

Wen-Tien Tsai

Toxics 2020, 8(3), 48; https://doi.org/10.3390/toxics8030048 - 07 Jul 2020

Abstract

In the past two decades, the waste electrical and electronic equipment (WEEE) management has become an important environmental issue internationally because it contained hazardous substances like heavy metals and brominated flame retardants. Moreover, some valuable substances were used in the electrical and electronic products, thus representing a circular industry for recycling of WEEE. Therefore, the Taiwan government formulated a legal WEEE recycling system since 1998 in response to the international trends of sustainable waste management and extended producer responsibility (EPR). This article adopted the national statistics in Taiwan regarding the online reporting amounts of collected WEEE since it has been officially designated as one of the mandatory recyclable wastes. Furthermore, the regulatory measures were addressed to update the status and subsidiary fee rates of WEEE recycling in Taiwan. In addition, this article also put emphasis on the regulations governing the toxic chemical substances contained in the WEEE. It showed that the average annual recycling amounts of home electronic appliances, information technology products and lighting in Taiwan during the 2017–2018 were around 117,000, 18,000 and 4500 metric tons, respectively. It was also indicated that the current WEEE recycling market in Taiwan has become saturated, reflecting the regulatory promulgation and promotional measures successfully. In response to the Stockholm Convention on persistent organic pollutants (POPs) and the Minamata Convention on Mercury, the Taiwan government declared some brominated flame retardants and heavy metals (i.e., mercury and cadmium) as a “toxic chemical substance” under the Toxic and Concerned Chemical Substance Control Act (TCCSCA), which shall be prohibited to use in the preparation of electrical and electronic equipment (EEE) since 1 January 2016. Through the central governing authority, local governments, and private recyclers in Taiwan, the successful WEEE recycling system not only reduce the pressure on sanitary disposal systems, but also prevent the chemical hazards from solid waste incineration systems. More significantly, the WEEE recycling in Taiwan echoed the United Nations (UN) Agenda 2030 for sustainable development goals. Full article

(This article belongs to the Special Issue Electronic-Waste: Management and Challenges)

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Open AccessArticle

Mercury Exposure and Associations with Hyperlipidemia and Elevated Liver Enzymes: A Nationwide Cross-Sectional Survey

Seungho Lee ,

Sungkyoon Kim and

Toxics 2020, 8(3), 47; https://doi.org/10.3390/toxics8030047 - 01 Jul 2020

Abstract

Mercury (Hg) has obesogenic properties. However, the associated health outcomes of population-level mercury exposure were unclear. This study investigated the relationships between blood mercury levels and obesity-related outcomes such as hyperlipidemia and elevated liver enzymes. Using the second cycle of the Korean National Environmental Health Survey (n = 6454), we performed logistic regression to examine the effects of Hg on hyperlipidemia and elevated liver enzymes. The blood mercury levels were significantly higher in the hyperlipidemia group (n = 3699, male: 4.03 μg/L, female: 2.83 μg/L) compared to the non-hyperlipidemia group (n = 2755, male: 3.48 μg/L, female: 2.69 μg/L), and high blood mercury levels were associated with an 11% higher risk of hyperlipidemia. The elevated liver enzymes group had higher mean blood mercury levels (n = 1189, male: 4.38 μg/L, female: 3.25 μg/L) than the normal group (n = 5265, male: 3.64 μg/L, female: 2.70 μg/L), and elevated blood mercury was associated with a 35% higher risk of elevated liver enzymes. Moreover, the effect was constant after adjusting for personal medications. These results indicate that mercury exposure is significantly associated with hyperlipidemia and elevated liver enzymes. Full article

(This article belongs to the Special Issue Toxic Metals, Chronic Diseases and Related Cancers)

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Open AccessArticle

Pulmonary Toxicity and Inflammatory Response of E-Cigarette Vape Cartridges Containing Medium-Chain Triglycerides Oil and Vitamin E Acetate: Implications in the Pathogenesis of EVALI

Thivanka Muthumalage ,

Joseph H. Lucas ,

Qixin Wang ,

Thomas Lamb ,

Matthew D. McGraw and

Irfan Rahman

Toxics 2020, 8(3), 46; https://doi.org/10.3390/toxics8030046 - 28 Jun 2020

Abstract

Recently, there has been an outbreak associated with the use of e-cigarette or vaping products, associated lung injury (EVALI). The primary components of vaping products, vitamin E acetate (VEA) and medium-chain triglycerides (MCT), may be responsible for acute lung toxicity. Currently, little information is available on the physiological and biological effects of exposure to these products. We hypothesized that these e-cig vape cartridges and their constituents (VEA and MCT) induce pulmonary toxicity, mediated by oxidative damage and inflammatory responses, leading to acute lung injury. We studied the potential mechanisms of e-cig vape cartridge aerosol induced inflammatory response by evaluating the generation of reactive oxygen species by MCT, VEA, and cartridges and their effects on the inflammatory state of pulmonary epithelium and immune cells both in vitro and in vivo. Cells exposed to these aerosols generated reactive oxygen species, caused cytotoxicity, induced epithelial barrier dysfunction, and elicited an inflammatory response. Using a murine model, the parameters of acute toxicity to aerosol inhalation were assessed. Infiltration of neutrophils and lymphocytes was accompanied by significant increases in IL-6, eotaxin, and G-CSF in the bronchoalveolar lavage fluid (BALF). In mouse plasma, eicosanoid inflammatory mediators, leukotrienes, were significantly increased. Plasma from e-cig users also showed increased levels of hydroxyeicosatetraenoic acid (HETEs) and various eicosanoids. Exposure to e-cig vape cartridge aerosols showed the most significant effects and toxicity compared to MCT and VEA. In addition, we determined SARS-CoV-2 related proteins and found no impact associated with aerosol exposures from these tested cartridges. Overall, this study demonstrates acute exposure to specific e-cig vape cartridges induces in vitro cytotoxicity, barrier dysfunction, and inflammation and in vivo mouse exposure induces acute inflammation with elevated proinflammatory markers in the pathogenesis of EVALI. Full article

(This article belongs to the Special Issue Current Knowledge of E-cigarettes and Heated Tobacco Products)

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Open AccessCommunication

Combining Different In Vitro Bioassays to Evaluate Genotoxicity of Water-Accommodated Fractions from Petroleum Products

Sarah Johann ,

Mira Goßen ,

Peter A. Behnisch ,

Henner Hollert and

Thomas-Benjamin Seiler

Toxics 2020, 8(2), 45; https://doi.org/10.3390/toxics8020045 - 26 Jun 2020

Abstract

Genotoxicity assessment is of high relevance for crude and refined petroleum products, since oil compounds are known to cause DNA damage with severe consequences for aquatic biota as demonstrated in long-term monitoring studies. This study aimed at the optimization and evaluation of small-scale higher-throughput assays (Ames fluctuation, micronucleus, Nrf2-CALUX^®) covering different mechanistic endpoints as first screening tools for genotoxicity assessment of oils. Cells were exposed to native and chemically dispersed water-accommodated fractions (WAFs) of three oil types varying in their processing degree. Independent of an exogenous metabolic activation system, WAF compounds induced neither base exchange nor frame shift mutations in bacterial strains. However, significantly increased chromosomal aberrations in zebrafish liver (ZF-L) cells were observed. Oxidative stress was indicated for some treatments and was not correlated with observed DNA damage. Application of a chemical dispersant increased the genotoxic potential rather by the increased bioavailability of dissolved and particulate oil compounds. Nonetheless, the dispersant induced a clear oxidative stress response, indicating a relevance for general toxic stress. Results showed that the combination of different in vitro assays is important for a reliable genotoxicity assessment. Especially, the ZF-L capable of active metabolism and DNA repair seems to be a promising model for WAF testing. Full article

(This article belongs to the Section Ecotoxicology)

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Open AccessArticle

Exposure Assessment of Cadmium in Female Farmers in Cadmium-Polluted Areas in Northern Japan

Yoko Hosoi ,

Akira Ono and

Fujio Kayama

Toxics 2020, 8(2), 44; https://doi.org/10.3390/toxics8020044 - 17 Jun 2020

Abstract

Akita prefecture is located in the northern part of Japan and has many cadmium-polluted areas. We herein performed an exposure assessment of cadmium in 712 and 432 female farmers in two adjacent cadmium-polluted areas (A and B, respectively), who underwent local health examinations from 2001–2004. We measured cadmium concentrations in 100 food items collected from local markets in 2003. We then multiplied the intake of each food item by its cadmium concentration in each subject to assess cadmium intake from food and summed cadmium intake from all food items to obtain the total cadmium intake. Median cadmium intake levels in areas A and B were 55.7 and 47.8 µg/day, respectively, which were both higher than that of the general population and were attributed to local agricultural products, particularly rice. We also calculated weekly cadmium intake per body weight and compared it to the previous provisional tolerable weekly intake reported by the Joint FAO (Food and Agriculture Organization)/WHO (World Health Organization) expert committee on food additives or current tolerable weekly intake in Japan of 7 µg/kg BW/week. Medians in areas A and B were 7.2 and 6.0 µg/kg BW/week, respectively. Similar estimated values were also obtained by the Monte Carlo simulation. These results demonstrated that the cadmium exposure levels among the farmers were high enough to be approximately the tolerable weekly intake. Full article

(This article belongs to the Special Issue Toxic Metals, Chronic Diseases and Related Cancers)

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Open AccessArticle

Per- and Polyfluoroalkyl Substances in Early Pregnancy and Risk for Preeclampsia: A Case-Control Study in Southern Sweden

Karin Broberg and

Toxics 2020, 8(2), 43; https://doi.org/10.3390/toxics8020043 - 16 Jun 2020

Abstract

Preeclampsia is one of the most common causes of perinatal and maternal morbidity/mortality. One suggested environmental risk factor is exposure to endocrine-disrupting pollutants such as per- and polyfluoroalkyl substances (PFAS). The present case-control study in southern Sweden aims to investigate the hypothesized association between serum concentrations of PFAS in early pregnancy and the risk of developing preeclampsia. The study included 296 women diagnosed with preeclampsia (cases) and 580 healthy pregnant women (controls). Maternal serum samples were obtained from a biobank of samples collected in early pregnancy in connection with screening for infections. Serum concentrations of perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), perfluorononanoic acid (PFNA), and perfluorohexane sulfonate (PFHxS) were analyzed using liquid chromatography-tandem-mass-spectrometry (LC/MS/MS). Among primiparous women, there were no differences in PFAS concentrations in early pregnancy between the cases and the controls whereas among multipara women, the cases had significantly higher concentrations of PFNA (median concentrations were 0.44 and 0.38 ng/mL, p = 0.04). When individual PFAS were categorized into quartiles and adjustment for potential confounders was performed, the women in the highest quartiles had no significant increased risks of developing preeclampsia as compared with women in the lowest category. In conclusion, the present study provides limited support for the hypothesized association between PFAS and preeclampsia in a population with relatively low exposure levels. Full article

(This article belongs to the Special Issue Health Effects Associated with Exposures to Endocrine Disrupting Chemicals)

Open AccessReview

Per- and Polyfluoroalkyl Substances (PFAS) Neurotoxicity in Sentinel and Non-Traditional Laboratory Model Systems: Potential Utility in Predicting Adverse Outcomes in Human Health

Rachel Foguth ,

Maria S. Sepúlveda and

Jason Cannon

Toxics 2020, 8(2), 42; https://doi.org/10.3390/toxics8020042 - 15 Jun 2020

Abstract

Per- and polyfluoroalkyl substances (PFAS) are a class of chemicals that were widely used in manufacturing and are now present in the environment throughout the world. It is known that various PFAS are quantifiable in human in blood, but potential adverse health outcomes remain unclear. Sentinel and non-traditional model species are useful to study potential toxicity of PFAS in order to understand the relationship between environmental and human health. Here, we present a critical review of studies on the neurotoxicity of PFAS in sentinel and non-traditional laboratory model systems, including Caenorhabditis elegans (nematode), Dugesia japonica (planarian), Rana pipiens (frogs), Danio rerio and Oryzias melastigma (fish), and Ursus maritimus (polar bears). PFAS have been implicated in developmental neurotoxicity in non-traditional and traditional model systems as well as sentinel species, including effects on neurotransmitter levels, especially acetylcholine and its metabolism. However, further research on the mechanisms of toxicity needs to be conducted to determine if these chemicals are affecting organisms in a similar manner. Overall, findings tend to be similar among the various species, but bioaccumulation may vary, which needs to be taken into account in future studies by quantifying target organ concentrations of PFAS to better compare different species. Furthermore, data on the majority of PFAS is lacking in neurotoxicity testing, and additional studies are needed to corroborate findings thus far. Full article

(This article belongs to the Special Issue Biological Fate and Potential Hazards of Per- and Polyfluorinated Alkyl Substances (PFAS))

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Open AccessFeature PaperReview

Conversion of Cannabidiol (CBD) into Psychotropic Cannabinoids Including Tetrahydrocannabinol (THC): A Controversy in the Scientific Literature

Constanze Sproll and

Toxics 2020, 8(2), 41; https://doi.org/10.3390/toxics8020041 - 03 Jun 2020

Abstract

Cannabidiol (CBD) is a naturally occurring, non-psychotropic cannabinoid of the hemp plant Cannabis sativa L. and has been known to induce several physiological and pharmacological effects. While CBD is approved as a medicinal product subject to prescription, it is also widely sold over the counter (OTC) in the form of food supplements, cosmetics and electronic cigarette liquids. However, regulatory difficulties arise from its origin being a narcotic plant or its status as an unapproved novel food ingredient. Regarding the consumer safety of these OTC products, the question whether or not CBD might be degraded into psychotropic cannabinoids, most prominently tetrahydrocannabinol (THC), under in vivo conditions initiated an ongoing scientific debate. This feature review aims to summarize the current knowledge of CBD degradation processes, specifically the results of in vitro and in vivo studies. Additionally, the literature on psychotropic effects of cannabinoids was carefully studied with a focus on the degradants and metabolites of CBD, but data were found to be sparse. While the literature is contradictory, most studies suggest that CBD is not converted to psychotropic THC under in vivo conditions. Nevertheless, it is certain that CBD degrades to psychotropic products in acidic environments. Hence, the storage stability of commercial formulations requires more attention in the future. Full article

(This article belongs to the Section Risk Assessment and Risk Management)

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Open AccessReview

Interaction of Environmental Pollutants with Microplastics: A Critical Review of Sorption Factors, Bioaccumulation and Ecotoxicological Effects

Albert Menéndez-Pedriza and

Joaquim Jaumot

Toxics 2020, 8(2), 40; https://doi.org/10.3390/toxics8020040 - 02 Jun 2020

Abstract

Microplastics have become one of the leading environmental threats due to their persistence, ubiquity and intrinsic toxic potential. The potential harm that microplastics impose on ecosystems varies from direct effects (i.e., entanglement and ingestion) to their ability to sorb a diversity of environmental pollutants (e.g., heavy metals, persistent organic compounds or pharmaceuticals). Therefore, the toxicological assessment of the combined effects of microplastics and sorbed pollutants can produce in biota is one of the hottest topics on the environmental toxicology field. This review aims to clarify the main impacts that this interaction could have on ecosystems by (1) highlighting the principal factors that influence the microplastics sorption capacities; (2) discussing the potential scenarios in which microplastics may have an essential role on the bioaccumulation and transfer of chemicals; and (3) reviewing the recently published studies describing toxicological effects caused by the combination of microplastics and their sorbed chemicals. Finally, a discussion regarding the need for a new generation of toxicological studies is presented. Full article

(This article belongs to the Special Issue Underexplored Chemical Interactions in Humans and Wildlife)

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Open AccessArticle

Sequential Extraction of Potentially Toxic Metals: Alteration of Method for Cu-Ni Polluted Peat Soil of Industrial Barren

Marina V. Slukovskaya ,

Irina P. Kremenetskaya ,

Svetlana V. Drogobuzhskaya and

Andrey I. Novikov

Toxics 2020, 8(2), 39; https://doi.org/10.3390/toxics8020039 - 02 Jun 2020

Abstract

An evaluation of fraction composition and transformation of metal compounds emitted by metal ore processing enterprises and accumulated in soils is crucial for assessing the environmental risks of pollution and ecosystem benefit of remediation. The aim of this study was to develop a suitable sequential fractional procedure for metal pollutants for the peat soils matrix in the impact zone of a Cu-Ni smelter. Three experiment series were performed: (a) the study of the effect of ammonium acetate buffer pH in the range of 3.7–7.8 on the soil metal extraction; (b) the study of the effect of additional volume and frequency of soil treatment with solutions on the content of water-soluble, ammonium acetate extractable, and 0.1 N HNO₃ extractable fractions; and, (c) the determination of the metal fraction composition in the modified technique. Soil treatment with ammonium acetate buffer with a pH range of 4.5–5.5 was the most appropriate for the determination of mobile compounds of Cu and other metals in highly polluted peat soil. Triple soil treatment with water and ammonium acetate is necessary for the complete extraction of the water-soluble and exchangeable fractions, respectively. Additionally, we propose a procedure of full extraction of the exchangeable metal fraction from peat soils while using single treatment with 0.1 N HNO₃. This scheme allows evaluating geochemical mobility of metals and current environmental harm of polluted soils with a high content of organic matter. Full article

(This article belongs to the Section Environmental Chemistry)

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Open AccessArticle

E-Cigarette Liquid Provokes Significant Embryotoxicity and Inhibits Angiogenesis

Ahmed Malki ,

Semir Vranic and

Ala-Eddin Al Moustafa

Toxics 2020, 8(2), 38; https://doi.org/10.3390/toxics8020038 - 27 May 2020

Abstract

E-cigarette smoking (ECS) is a new method of tobacco smoking that is gaining popularity as it is thought to be a “healthy method” of tobacco consumption. The adverse outcomes of ECS on the respiratory and cardiovascular systems in humans have been recently demonstrated. Nevertheless, the effect of e-cigarette liquid (ECL) on the early stage of embryogenesis and angiogenesis has not been explored yet. Chicken embryo at 3 days of incubation and its chorioallantoic membrane (CAM) of 5 days were used to explore the outcome of ECL on the embryo. Real-time PCR was also employed to study the regulation of a set of key controller genes of embryogenesis as well as angiogenesis. Our study revealed that ECL exposure is associated with a high rate of mortality in embryos as around 70% of treated embryos, at 3 days of incubation, die after 5 days of exposure. Additionally, ECL inhibits angiogenesis of the CAM of 5 days of incubation by more than 30%. These effects could be explained by the upregulation of ATF-3, FOXA2, INHBA, MAPRE-2, and RIPK-1, as well as the downregulation of SERPINA-4 and VEGF-C genes, which are important key controller genes of embryogenesis as well as angiogenesis. Our data suggest clearly that ECS can have dramatic toxic outcomes on the early stage of embryogenesis as well as angiogenesis. Accordingly, we believe that further studies to assess the effects of ECS on human health are essential. Full article

(This article belongs to the Special Issue Current Knowledge of E-cigarettes and Heated Tobacco Products)

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Open AccessEditorial

Biomarkers of Environmental Toxicants: Exposure and Biological Effects

Robert J. Turesky and

Kun Lu

Toxics 2020, 8(2), 37; https://doi.org/10.3390/toxics8020037 - 22 May 2020

Abstract

Biomarkers of environmental toxicants are measures of exposures and effects, some of which can serve to assess disease risk and interindividual susceptibilities [...] Full article

(This article belongs to the Special Issue Biomarkers of Environmental Toxicants)

Open AccessArticle

Effects of Co-Contamination of Microplastics and Cd on Plant Growth and Cd Accumulation

Fayuan Wang ,

Xiaoqing Zhang ,

Shuqi Zhang ,

Shuwu Zhang ,

Catharine A. Adams and

Yuhuan Sun

Toxics 2020, 8(2), 36; https://doi.org/10.3390/toxics8020036 - 20 May 2020

Abstract

Microplastics (MPs) occur widely in terrestrial ecosystems. However, information on the interaction of MPs with metals in terrestrial ecosystems is lacking in the literature. The present study investigated the effects of two types of MPs (high-density polyethylene (HDPE) and polystyrene (PS)) with different dosages (i.e., 0, 0.1%, 1%, and 10%) on the uptake and effects of Cd in maize plants grown in an agricultural soil. Results showed that addition of Cd at a 5 mg/kg caused inhibited plant growth and resulted in high Cd accumulation in plant tissues. Polyethylene alone showed no significant phytotoxic effects, but a high-dose of HDPE (10%) amplified Cd phytotoxicity. Polystyrene negatively affected maize growth and phytoxicity further increased in the presence of Cd. Both HDPE and PS caused soil diethylenetriaminepentaacetic acid (DTPA)-extractable Cd concentrations to increase but did not significantly affect Cd uptake into plant tissues. In the soil without Cd addition, HDPE decreased soil pH, while PS did not significantly alter soil pH. However, in the soil spiked with Cd, both HDPE and PS increased pH. Overall, impacts on plant growth and Cd accumulation varied with MP type and dose, and PS induced substantial phytotoxicity. In conclusion, co-occurring MPs can change Cd bioavailability, plant performance, and soil traits. Our findings highlight the ecological impacts that could occur from the release of MPs into soil. Full article

(This article belongs to the Section Ecotoxicology)

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Open AccessArticle

Occurrence of Microplastic Pollution at Oyster Reefs and Other Coastal Sites in the Mississippi Sound, USA: Impacts of Freshwater Inflows from Flooding

Tarun Anumol and

Toxics 2020, 8(2), 35; https://doi.org/10.3390/toxics8020035 - 15 May 2020

Abstract

Much of the seafood that humans consume comes from estuaries and coastal areas where microplastics (MPs) accumulate, due in part to continual input and degradation of plastic litter from rivers and runoff. As filter feeders, oysters (Crassostrea virginica) are especially vulnerable to MP pollution. In this study, we assessed MP pollution in water at oyster reefs along the Mississippi Gulf Coast when: (1) historic flooding of the Mississippi River caused the Bonnet Carré Spillway to remain open for a record period of time causing major freshwater intrusion to the area and deleterious impacts on the species and (2) the spillway was closed, and normal salinity conditions resumed. Microplastics (~25 µm–5 mm) were isolated using a single-pot method, preparing samples in the same vessel (Mason jars) used for their collection right up until the MPs were transferred onto filters for analyses. The MPs were quantified using Nile Red fluorescence detection and identified using laser direct infrared (LDIR) analysis. Concentrations ranged from ~12 to 381 particles/L and tended to decrease at sites impacted by major freshwater intrusion. With the spillway open, average MP concentrations were positively correlated with salinity (r = 0.87, p = 0.05) for sites with three or more samples examined. However, the dilution effect on MP abundances was temporary, and oyster yields suffered from the extended periods of lower salinity. There were no significant changes in the relative distribution of MPs during freshwater intrusions; most of the MPs (>50%) were in the lower size fraction (~25–90 µm) and consisted mostly of fragments (~84%), followed by fibers (~11%) and beads (~5%). The most prevalent plastic was polyester, followed by acrylates/polyurethanes, polyamide, polypropylene, polyethylene, and polyacetal. Overall, this work provides much-needed empirical data on the abundances, morphologies, and types of MPs that oysters are exposed to in the Mississippi Sound, although how much of these MPs are ingested and their impacts on the organisms deserves further scrutiny. This paper is believed to be the first major application of LDIR to the analysis of MPs in natural waters. Full article

(This article belongs to the Special Issue Prevalence, Fate and Effects of Plastic in Freshwater Environments)

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Open AccessArticle

Effect of Exposure of Plastic Infant Feeding Bottle Leached Water on Biochemical, Morphological and Oxidative Stress Parameters in Rats

Monika Pandey and

Toxics 2020, 8(2), 34; https://doi.org/10.3390/toxics8020034 - 13 May 2020

Abstract

Bisphenol A (BPA) is leached out from plastic infant feeding bottles that are filled with warm milk/water due to high temperatures, exposing the infants to BPA. The present study aims to understand the effects of ingestion of BPA leached from plastic infant feeding bottle and delineate the underlying mechanisms in rats. In this study, adult rats of Wistar strain were divided into 3 groups. In the first group, the rats consumed normal food and tap water ad libitum. In the second group, the rats ingested BPA (20 µg/kg bodyweight/day, orally). In the third group, the rats drank water leached from plastic infant feeding bottles. After 30days, tests involving biochemical parameters, histopathological examination, and oxidative stress enzyme markers were performed, and the levels of BPA in plastic-leached water were estimated by HPLC analysis. There were significant biochemical changes in the form of increased alkaline phosphatase (ALP), creatine kinase-muscle/brain (CK-MB), and lactate dehydrogenase (LDH) levels in both treated groups as compared to control group, accompanied by structural damage to the vital organs, and lipid peroxidation, glutathione reductase, and catalase activity were also high in the treated groups. Further, the BPA concentration in plastic leached water was estimated to be 0.1 ± 0.02 µg/mL. Full article

(This article belongs to the Section Toxicology and Public Health)

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Open AccessArticle

A Phenotypic and Genotypic Evaluation of Developmental Toxicity of Polyhexamethylene Guanidine Phosphate Using Zebrafish Embryo/Larvae

Jeongah Song ,

Kojo Eghan ,

Sangwoo Lee ,

Jong-Su Park ,

Seokjoo Yoon ,

Wittaya Pimtong and

Woo-Keun Kim

Toxics 2020, 8(2), 33; https://doi.org/10.3390/toxics8020033 - 02 May 2020

Abstract

Polyhexamethylene guanidine-phosphate (PHMG-P), a guanidine-based cationic antimicrobial polymer, is an effective antimicrobial biocide, potent even at low concentrations. Due to its resilient bactericidal properties, it has been used extensively in consumer products. It was safely used until its use in humidifiers led to a catastrophic event in South Korea. Epidemiological studies have linked the use of PHMG-P as a humidifier disinfectant to pulmonary fibrosis. However, little is known about its harmful impacts other than pulmonary fibrosis. Thus, we applied a zebrafish embryo/larvae model to evaluate developmental and cardiotoxic effects and transcriptome changes using RNA-sequencing. Zebrafish embryos were exposed to 0.1, 0.2, 0.3, 0.4, 0.5, 1, and 2 mg/L of PHMG-P from 3 h to 96 h post fertilization. 2 mg/L of PHMG-P resulted in total mortality and an LC₅₀ value at 96 h was determined at 1.18 mg/L. Significant developmental changes were not observed but the heart rate of zebrafish larvae was significantly altered. In transcriptome analysis, immune and inflammatory responses were significantly affected similarly to those in epidemiological studies. Our qPCR analysis (Itgb1b, TNC, Arg1, Arg2, IL-1β, Serpine-1, and Ptgs2b) also confirmed this following a 96 h exposure to 0.4 mg/L of PHMG-P. Based on our results, PHMG-P might induce lethal and cardiotoxic effects in zebrafish, and crucial transcriptome changes were linked to immune and inflammatory response. Full article

(This article belongs to the Special Issue Contaminant Effects on Zebrafish Embryos)

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Open AccessArticle

Ecological and Human Health Risks of Heavy Metals in Shooting Range Soils: A Meta Assessment from China

Juan Bai and

Xiaofen Zhao

Toxics 2020, 8(2), 32; https://doi.org/10.3390/toxics8020032 - 01 May 2020

Abstract

Contamination of shooting ranges by heavy metals in particular Pb represents a widespread environmental issue attracting concern worldwide. Contaminant accumulation in shooting range soils can pose potential ecological risks and health risks for shooters and workers. Based on the published data on metal contamination at five shooting ranges in China, potential ecological and human health risks of several metals, and in particular, Pb were assessed for the five surveyed shooting ranges. Data show the mean concentrations of Pb, Cu, Hg, Sb, Ni and Cr in various ranges were all higher than the local soil background values, implying their accumulation was induced by shooting activities. The degree of contamination varied with sites and metals, very high Pb contamination at Range 1, Range 2 and Range 5-1, while moderate Pb contamination at Range 3 and Range 5-2. Comparatively, As, Zn and Co showed no contamination. Among the surveyed metals, Pb, Cu, Hg and Sb in shooting range soils displayed relatively high potential ecological risks. The overall degree of potential ecological risk was very high at Range 1 and Range 2, considerable at Range 4 and Range 5-1, and low at Range 3 and Range 5-2. The mean HI (hazard index) of Pb at Range 2 and the maximum HI values at Range 1 and Range 4 were higher than 1, suggesting a possibility of non-carcinogenic risks of Pb contamination at these sites. However, Pb in other range soils and other metals, across the five ranges, all exhibited no non-carcinogenic risks. The cancer risks of the four carcinogenic contaminants (As, Co, Cr, and Ni) were acceptable or negligible at all ranges. In conclusion, contamination of Pb and other metals such as Cu, Hg and Sb can cause various potential ecological risks at all the surveyed ranges, but only Pb at three ranges shows possible health risks. Contamination of Pb in the surveyed shooting ranges should be managed to reduce its possible environmental and health risks. Full article

(This article belongs to the Section Risk Assessment and Risk Management)

Open AccessArticle

Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

Alejandro Monserrat García-Alegría ,

Agustín Gómez-Álvarez ,

Iván Anduro-Corona ,

Armando Burgos-Hernández ,

Eduardo Ruíz-Bustos ,

Rafael Canett-Romero ,

Humberto González-Ríos ,

José Guillermo López-Cervantes ,

Karen Lillian Rodríguez-Martínez and

Humberto Astiazaran-Garcia

Toxics 2020, 8(2), 31; https://doi.org/10.3390/toxics8020031 - 20 Apr 2020

Abstract

Recently, soluble forms of aluminum for human use or consumption have been determined to be potentially toxic due to their association with hepatic, neurological, hematological, neoplastic, and bone conditions. This study aims to assess the genotoxic effect of aluminum chloride on genomic instability associated with the onset of N-nitroso-N-methylurea (NMU)-induced breast cancer in Sprague Dawley rats. The dietary behavior of the rats was assessed, and the concentration of aluminum in the mammary glands was determined using atomic absorption spectroscopy. Genomic instability was determined in the histological sections of mammary glands stained with hematoxylin and eosin. Moreover, micronucleus in peripheral blood and comet assays were performed. The results of dietary behavior evaluation indicated no significant differences between the experimental treatments. However, aluminum concentration in breast tissues was high in the +2000Al/−NMU treatment. This experimental treatment caused moderate intraductal cell proliferation, lymph node hyperplasia, and serous gland adenoma. Furthermore, micronucleus and comet test results revealed that +2000Al/−NMU led to a genotoxic effect after a 10-day exposure and the damage was more evident after a 15-day exposure. Therefore, in conclusion, genomic instability is present and the experimental conditions assessed are not associated with breast cancer. Full article

(This article belongs to the Special Issue Toxic Metals, Chronic Diseases and Related Cancers)

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Open AccessArticle

Theoretical Modeling of Oral Glucose Tolerance Tests Guides the Interpretation of the Impact of Perinatal Cadmium Exposure on the Offspring’s Glucose Homeostasis

Alexandre Rocca ,

Eric Fanchon and

Jean-Marc Moulis

Toxics 2020, 8(2), 30; https://doi.org/10.3390/toxics8020030 - 15 Apr 2020

Abstract

Oral glucose tolerance tests, in which the concentration of glucose is monitored in the circulation over 2 h after ingesting a bolus, probe diabetic or pre-diabetic conditions. The resulting glucose curves inform about glucose turnover, insulin production and sensitivity, and other parameters. However, extracting the relevant parameters from a single complex curve is not straightforward. We propose a simple modeling method recapitulating the most salient features of the role of insulin-secreting pancreatic

β

-cells and insulin sensitive tissues. This method implements four ordinary differential equations with ten parameters describing the time-dependence of glucose concentration, its removal rate, and the circulating and stored insulin concentrations. From the initial parameter set adjusted to a reference condition, fitting is done by minimizing a weighted least-square residual. In doing so, the sensitivity of

β

-cells to glucose was identified as the most likely impacted function at weaning for the progeny of rats that were lightly exposed to cadmium in the perigestational period. Later in life, after young rats received non-contaminated carbohydrate enriched food, differences are more subtle, but modeling agrees with long-lasting perturbation of glucose homeostasis. Full article

(This article belongs to the Special Issue Toxic Metals, Chronic Diseases and Related Cancers)

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Open AccessArticle

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Evane Thorel ,

Fanny Clergeaud ,

Lucie Jaugeon ,

Alice M. S. Rodrigues ,

Julie Lucas ,

Didier Stien and

Philippe Lebaron

Toxics 2020, 8(2), 29; https://doi.org/10.3390/toxics8020029 - 10 Apr 2020

Abstract

The presence of pharmaceutical and personal care product (PPCP) residues in the aquatic environment is an emerging issue due to their uncontrolled release through gray water, and accumulation in the environment that may affect living organisms, ecosystems and public health. The aim of this study is to assess the toxicity of benzophenone-3 (BP-3), bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT), butyl methoxydibenzoylmethane (BM), methylene bis-benzotriazolyl tetramethylbutylphenol (MBBT), 2-ethylhexyl salicylate (ES), diethylaminohydroxybenzoyl hexyl benzoate (DHHB), diethylhexyl butamido triazone (DBT), ethylhexyl triazone (ET), homosalate (HS) and octocrylene (OC) on marine organisms from two major trophic levels, including autotrophs (Tetraselmis sp.) and heterotrophs (Artemia salina). In general, results showed that both HS and OC were the most toxic UV filters for our tested species, followed by a significant effect of BM on Artemia salina due to BM—but only at high concentrations (1 mg/L). ES, BP3 and DHHB affected the metabolic activity of the microalgae at 100 µg/L. BEMT, DBT, ET, MBBT had no effect on the tested organisms, even at high concentrations (2 mg/L). OC toxicity represents a risk for those species, since concentrations used in this study are 15–90 times greater than those reported in occurrence studies for aquatic environments. For the first time in the literature, we report HS toxicity on a microalgae species at concentrations complementing those found in aquatic environments. These preliminary results could represent a risk in the future if concentrations of OC and HS continue to increase. Full article

(This article belongs to the Section Ecotoxicology)

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