Poultry, Volume 5, Issue 2 (April 2026) – 15 articles

The manual handling of poultry is an essential part of raising and caring for birds. The different manual catching methods have various impacts on the bird’s welfare and health, the well-being and work satisfaction of the people who are handling the birds, and the economic and logistical requirements of everyone involved. The traditional approach of using the Five Freedoms for investigating animal well-being has been amended with animal-based measures (ABMs) as well as the evaluation of five welfare domains, which consider the subjective response of an animal towards its environment. The assessment of single individual animal welfare parameters without context can be non-specific, only partially informative, or even misleading when considered in isolation. The objective measurement of suitable parameters for the evaluation of the various steps of poultry catching and transport is complex and should be carried out in a differentiated manner. This review summarizes the current knowledge about the manual catching of poultry, with special focus on the upright and inverted handling of chicken and current considerations in Europe. The implementation of consistent, transparent, and traceable central data collection on animal health and welfare at various critical control points of bird transportation would allow systematic evaluation of the multifactorial welfare assessment in the future. Full article

The global restriction of antimicrobial growth promoters has intensified the search for alternative strategies to sustain poultry health and productivity. One proposed mechanism underlying the historical efficacy of antibiotic performance enhancers is the modulation of intestinal inflammation. In this context, meloxicam (MLX), a preferential COX-2 inhibitor and non-steroidal anti-inflammatory drug, has emerged as a potential candidate for investigation. However, pharmacokinetic data in broiler chickens remain limited, particularly for practical oral formulations intended for production systems. This study aimed to characterize the pharmacokinetic profile of a novel granulated MLX formulation in male Cobb 500 broiler chickens following single-dose administration. Seventy-two 21-day-old broilers received MLX granulate (19.24% m/m) via oral gavage at 3.6 mg/kg body weight. Plasma samples were collected over 48 h post administration. MLX concentrations were quantified using validated high-performance liquid chromatography, and pharmacokinetic parameters were estimated using nonlinear mixed-effects modelling (NLME). Mean pharmacokinetic parameters included AUC_0–∞ of 79.97 μg·h/mL, C_max of 14.43 μg/mL, and T_max of 1 h, indicating rapid absorption and substantial systemic exposure. These findings provide novel insights into MLX disposition from the granulated formulation in broilers and provide pharmacokinetic information to support future investigations evaluating its potential biological effects in poultry production systems. Full article

This study aimed to evaluate the potential of Lonicera japonica Flos (LJF) as an alternative agent against Eimeria tenella (E. tenella) in chickens and to conduct phytochemical analysis to obtain compositional insights. Seventy-two one-day-old chickens were allocated to six groups and fed diets supplemented with LJF powder (LJFp) at three concentrations (LJFp-L, LJFp-M, and LJFp-H) or maduramicin (MDM) or two non-supplemented control diets, namely, an infected unmedicated control (IUC) and an uninfected unmedicated control (UUC). Dietary treatments were initiated at chick arrival (Day 0) and continued for 28 days. At 21 days of age, all groups except the UUC group were orally challenged with a field isolate of E. tenella (PT-Te003; 2.0 × 10⁴ oocysts/bird). Anticoccidial efficacy was assessed using the lesion score (LS), oocysts per gram of feces (OPG), relative body weight gain (rBWG), and anticoccidial index (ACI). The results demonstrated that all LJFp treatment groups had significantly reduced cecal OPG and LS (all LJFp treatments: p < 0.05 vs. IUC), indicating the effective suppression of E. tenella replication and intestinal damage. Regarding growth performance, the rBWG values of the LJFp-L and LJFp-M groups were the highest and comparable to those of the UUC group, showing no significant differences. In contrast, the LJFp-H and MDM groups exhibited significantly lower values (p < 0.05). Based on ACI evaluation, all LJFp-treated groups exhibited moderate to partial efficacy (LJFp-L > LJFp-M > LJFp-H), while MDM showed limited effectiveness. A gas chromatography–mass spectrometry (GC-MS) analysis of the LJFp ethanol extract revealed 15 essential oils, 10 organic acids, and three other compound classes, several of which have been associated with anticoccidial activity. Overall, the in vivo results suggest that LJF may exert potential anticoccidial effects against a field isolate of E. tenella. Phytochemical analysis provided preliminary compositional insights, and further studies are warranted to optimize extraction methods and evaluate efficacy at lower concentrations under additional in vitro and in vivo conditions. However, the current evidence remains insufficient to determine whether the field isolate exhibits reduced sensitivity to commercially available anticoccidial drugs, and additional studies are needed to clarify this issue. Full article

Backyard chicken farming is a longstanding practice in Romania, with over 13 million birds raised in such systems to provide households with essential sources of eggs and meat. Despite advantages such as low production costs and the potential for organic husbandry, backyard poultry remains highly vulnerable to parasitic infections due to limited biosecurity measures and minimal regulatory oversight. This study aimed to evaluate the efficacy of a plant-based anthelmintic product in naturally infected backyard flocks. Among the 35 backyard farms examined, 24 (68.6%) tested positive for helminth infections, with prevalence rates of 51.4% for Ascaridia galli/Heterakis gallinarum, 57.1% for Capillaria spp., and 40% for Strongyloides avium. Positive households were assigned to three groups: untreated control, fenbendazole-treated, and herbal product-treated. The plant-based formulation consisted of alcoholic extracts from seven locally sourced plants, Thymus serpyllum, Calendula officinalis, Inula helenium, Tanacetum vulgare, Origanum vulgare, Artemisia annua, and Artemisia absinthium, and was administered via drinking water at a dose of 5 mL/L for seven consecutive days. Anthelmintic efficacy was assessed using the fecal egg count reduction test (FECRT). Fecal samples were collected before treatment and on days 7, 14, and 21 post-treatment, and examined using flotation and the McMaster technique. Both the plant-based and fenbendazole-treated groups exhibited significant reductions in egg counts for A. galli/H. gallinarum (p ≤ 0.00001), although neither treatment reached the 90% efficacy threshold required for reliable parasitic control in poultry. The herbal formulation achieved a 91.33% reduction in fecal egg counts overall and a complete (100%) efficacy against Strongyloides avium at 7 and 21 days post-treatment, respectively. At 14 days post-treatment, the mean FECRT values across all parasite species ranged from 13.64% in the untreated control group to 71.86% in the fenbendazole-treated group and 67.11% in the herbal-treated group. The highest reduction was observed in the fenbendazole group against Ascaridia galli/Heterakis gallinarum (85.22 ± 11.86), while the lowest was recorded in the control group against S. avium (–31.63 ± 96.64). Overall, our findings indicate that plant-based anthelmintics are a promising, environmentally friendly option for organic backyard poultry systems, offering a viable alternative to conventional chemical treatments. Full article

Efficient liquid storage of turkey semen is critical for artificial insemination, but its use is limited by bacteriospermia and oxidative damage. This study evaluated the effects of gentamicin supplementation in Glutac and Sperm Motility Medium (SMM) on bacterial load and sperm quality after 2 and 24 h of liquid storage. Semen from turkeys (n = 40) was assessed for motility, viability, plasma membrane and acrosome integrity, mitochondrial and metabolic activity, oxidative profile, apoptosis, DNA integrity, and microbiological status. The sperm motility and kinematic parameters declined significantly after 24 h in all the groups. However, both extenders (particularly SMM) maintained significantly higher motility than the untreated control. Gentamicin further improved the motility, viability, and plasma membrane and acrosome integrity. The mitochondrial activity and mitochondrial membrane potential were significantly higher in the extender-treated groups than in the controls at 2 and 24 h, whereas the superoxide and total ROS production were significantly higher in the controls. The total antioxidant capacity declined markedly in the untreated controls, especially after 24 h. Gentamicin significantly reduced bacterial load, most effectively in SMM, and decreased DNA fragmentation compared with the untreated controls. In conclusion, gentamicin supplementation—particularly in SMM—reduces bacteriospermia and oxidative stress while preserving turkey sperm quality during liquid storage. Full article

In recent years, outbreaks of inclusion body hepatitis (IBH) have been reported with increasing frequency worldwide. In Austria, the presence of fowl aviadenoviruses (FAdVs) and/or IBH has been documented sporadically. The present report describes a surge of natural IBH cases in fifteen Ross 308 broiler flocks. The clinical picture was characterized by the sudden onset of increased mortality in birds 3 to 15 days of age, persisting for approximately one week, resulting in cumulative flock mortality ranging from 2% to 27%. The main pathological findings were hepatitis with diffuse necrotic foci and petechial hemorrhages with intranuclear inclusion bodies by histology. FAdV was detected in liver samples from all affected flocks. Based upon partial hexon sequence analysis, FAdV serotype-11, species-D (FAdV-11, FAdV-D) was identified, showing complete sequence identity among broiler-derived strains and high identity with previous isolates from Austria and globally IBH-associated isolates. Furthermore, FAdV-11 DNA was detected in dead-in-shell embryos collected during the ongoing outbreak, originating from one suspect broiler breeder flock, confirming vertical transmission. Serological investigations of the suspect breeders confirmed the FAdV-11 infections within the studied timeframe. In conclusion, investigations confirmed vertical transmission of FAdV-11 and subsequent manifestation of IBH in broilers under field conditions, highlighting the epidemiological importance of broiler breeders in IBH outbreaks. Full article

Global population growth is expected to increase poultry meat demand, intensifying the need for sustainable protein sources. Soybean meal, the primary protein feed for poultry, has negative associations with deforestation and long transport distances. Duckweed has emerged as a possible, more sustainable alternative due to its high growth rate and protein yield. The nutrient digestibility and performance effects of the duckweed species Lemna minor (L. minor) in broiler diets were investigated in two experiments. Experiment 1 determined the ileal digestibility of crude protein, amino acids, phosphorus, and metabolizable energy in L. minor. The digestibility of most amino acids in L. minor ranged from 70% to 96%, with lysine and methionine at 87% and 86%, respectively. At 48%, the digestibility of cysteine was markedly lower than that of the other amino acids. However, the digestibility of P exceeded 90%. The energy values of dry matter were 7.05 MJ AME and 6.13 MJ. Experiment 2 tested the inclusion of L. minor (up to 10%) in isoenergetic and isonitrogenous diets. No significant effects on nutrient digestibility, weight gain, feed intake, or feed conversion ratio were observed. Both experiments demonstrate that L. minor cultivated under controlled conditions is a highly digestible, reliable feed source. Its inclusion in broiler diets is feasible, as it does not impair performance, yet provides amino acid balance whilst ensuring biomass quality. These findings support L. minor as a novel protein alternative and warrant further research on higher inclusion rates. Full article

Sexual dimorphism is a well-recognized factor influencing growth performance and meat quality in poultry; however, its effects remain less explored in Muscovy ducks. This study investigated the effect of sex on carcass traits, meat quality parameters, muscle fiber characteristics, and lipid composition in 14-week-old Muscovy ducks. Carcass dissection, physical and chemical meat analyses, and histological evaluation of breast muscle were performed in males and females. Sex markedly affected carcass performance, with males exhibiting higher live, slaughter, semi-eviscerated, and eviscerated weights, whereas females showed greater abdominal fat deposition and higher drip and processing losses, indicating reduced water-holding capacity. In the breast muscle, females displayed lower ultimate pH, higher yellowness (b*), and lower shear force values, suggesting more tender meat. Chemical composition differed between sexes, with males showing higher moisture and protein contents and lower intramuscular fat, accompanied by sex-related differences in fatty acid composition. Muscle fiber morphology also varied significantly, as males exhibited larger fiber diameters and cross-sectional areas in the breast muscles. Overall, these findings demonstrate that sex is a critical determinant of carcass yield, technological meat properties, and nutritional quality in Muscovy ducks, highlighting its importance for targeted production and processing strategies. Full article

Broiler production efficiency depends on the capacity of different strains to utilize nutrients and minimize environmental losses. This study aimed to quantify the excretion and digestibility of dry matter, nitrogen, and phosphorus in Cobb 500, Ross 308, and Hubbard Flex strains at different ages under Southwest Piauí conditions. A total of 300 broilers of both sexes were distributed in a 3 × 2 factorial design (3 strains × 2 sexes) with five replicates, totaling 30 experimental units. The six-week trial included adaptation periods, excreta collection, and feed intake control each week. Dry matter, nitrogen, and phosphorus were analyzed in diets and excreta to calculate intake, excretion, and retention coefficients (DMR, NR, PR). Interactions between sex and strain were observed after 25 days for some variables. Males excreted 10.21% more phosphorus than females, and strain effects were mainly observed for phosphorus excretion, with Hubbard exhibiting lower excretion in the 32–35-day phase, Cobb presenting the highest, and Ross demonstrating intermediate excretion. Variations between sexes were observed within strains across ages. These results demonstrate that nutrient utilization is influenced by both strain and sex, highlighting the need for tailored nutritional strategies to improve feed efficiency and reduce environmental impact in broiler production. Full article

Over the years, poultry supply chains have prioritized highly productive genetic lines to meet consumer demand, often at the expense of meat quality, animal welfare, and animal health. Recently, however, industry trends have shifted toward a greater awareness of welfare, reduced farming intensity, and improved product quality. In response, the European Chicken Commitment (ECC) has advocated for the use of slower-growing genotypes, even within conventional production systems. This study aimed to evaluate the meat quality of two ECC-approved chicken genotypes with differing growth rates—slow-growing (SG: 30–40 g/day, Kabir) and medium-growing (MG: 40–50 g/day, Ranger Gold) in comparison with a fast-growing strain (FG: >65 g/day, Ross 308). A total of 300 chickens were assigned to two experimental conditions: a control group (C), with spontaneous activity, and a treatment group (M), subjected to induced moderate kinetic activity. The results demonstrated that genotype influenced the meat quality of chickens raised indoors more significantly than kinetic activity. Comparisons revealed that SG and MG chickens exhibited superior meat quality, particularly regarding protein content, oxidative status, and a more suitable fatty acid profile. Overall, our findings support the adoption of ECC-approved genotypes in indoor systems to simultaneously improve animal welfare and enhance the nutritional and technological quality of poultry meat. Full article

High-quality, well-balanced feeding strategies are essential for optimizing poultry growth performance and for ensuring the safety of poultry products. Here, we evaluated the effects of chelated vs. inorganic trace minerals on the growth, survival and carcass characteristics of Cobb 500 broiler chickens. A large-scale feeding trial involved four dietary treatments at 21–35 days of age, with Group 1 receiving a control diet with mineral supplements of zinc (Zn), copper (Cu) and manganese (Mn) in sulfate form, based on a standard formulation. A further three experimental diets contained chelated forms of Zn (Group 2), Cu (Group 3) and a mineral premix MINTREX^® that provides Zn, Cu and Mn (Group 4). Broilers were raised to 42 days of age under commercial production conditions. Feeding chelated trace minerals resulted in significantly higher body weights during the rearing period, including a 10% increase in pre-slaughter weight compared to the controls (p < 0.01). Moreover, survival rates improved from 87% in the controls to 95–96% in the treatment groups (p < 0.001), and carcass evaluation revealed a 15–17% increase in eviscerated carcass weight in birds fed chelated supplements (p < 0.05). Greater yields of muscle, liver and skin with subcutaneous fat were observed, as was improved production of total edible carcass components. Among the treatments, MINTREX^® provided the greatest enhancement effects in performance and slaughter traits. These findings demonstrate that dietary chelated minerals, whether single or combined, may have positive effects on the broiler carcass yield and support their inclusion in poultry production systems. Full article

A two-cohort feasibility study was conducted to investigate the potential role of homeopathic therapy in reducing antimicrobial use in turkeys. The objective of the analysis presented here was to describe the complex characteristics of the study farms. For analysis of heterogeneity, data of 25 turkey farms (7 homeopathy (H); 18 conventional (C)) were analyzed. Farms in cohort H had significantly higher numbers of poultry farms nearby and included outdoor husbandry, while those in cohort C included neither organic nor biodynamic husbandry types. During raising, a combination of straw and wood shavings was more frequent, while during fattening, only one type of litter was predominant. Very few additional significant differences were identified at farm level, while at production batch level, several further differences existed. When comparing the study cohort characteristics with national statistics, both cohorts seemed to perform better than the national average. The incidence of antibiotic treatment days was lower in cohort H compared to cohort C (C 0.14, H 0.01, p < 0.001), while production period-related mortality (C 3.7%, H 3.7%) was comparable. Our detailed collection of data, previously unavailable, provides a relevant insight and will enable the analysis of multivariable aspects linked to antimicrobial usage in turkey production. Full article

The poultry red mite Dermanyssus gallinae (PRM) is a hematophagous ectoparasite of major veterinary and public health concern, recognized as a potential vector of zoonotic pathogens. Despite recent advances in characterizing its microbiota, the temporal dynamics of the microbial community remain poorly understood. Here, we conducted a longitudinal metabarcoding survey of engorged PRM collected from a commercial cage-free laying hen farm over the laying hen’s productive cycle (30–105 weeks). High-throughput sequencing of the 16S rRNA V3–V4 region generated 412,078 sequences, identifying 186 bacterial species across all samples. Microbial richness peaked at 30 weeks (164 species), but sharply declined thereafter, with only 28, 55, and 43 species detected at 60, 90, and 105 weeks, respectively. Ordination (NMDS) and PERMANOVA analyses revealed significant temporal restructuring of microbial communities (R² = 0.76, p < 0.01), with distinct clustering across sampling points. A small subset of taxa persisted throughout time, including the genera Bartonella and Rickettsiella, while many species exhibited transient or stage-specific occurrence. Notably, zoonotic pathogens such as Staphylococcus aureus, Kocuria massiliensis, and Bartonella vinsonii were detected, suggesting that PRM may harbor microorganisms of potential medical and veterinary relevance. Overall, our findings demonstrate that PRM harbors a highly diverse but temporally dynamic microbiota, which progressively contracts into a community dominated by stable symbionts. These results highlight critical windows for microbial succession and reinforce the relevance of microbiome-based surveillance and integrated control strategies within a One Health framework. Full article

Zoonotic Salmonella strains are major pathogens causing foodborne illness, and poultry products are among the main sources of infection. Biosecurity on poultry farms is central to preventing the introduction of Salmonella; together with vaccination, it can reduce the risk of meat and eggs becoming contaminated. Live Salmonella vaccines are conveniently administered via drinking water, and good vaccination practices are essential to ensure flock protection. This requires drinking water to be compatible with live vaccines. Data about the impact of water quality on vaccine viability are limited; therefore, this study investigated the impact of different water parameters on the stability of a bivalent live Salmonella vaccine. In addition, the impact of the most common disinfectants used for drinking water sanitization was assessed. Fluoride, nitrate, sulphate levels, and hardness had little impact on vaccine survival, while aluminium, arsenic, iron, and manganese levels had a pronounced, dose-dependent effect. pH value, conductivity, and chloride levels impacted the vaccine stability only at high levels. Free chlorine, chlorine dioxide, and hydrogen peroxide strongly reduced the viability of the vaccine, even though a water stabilizer offered protection from chlorine and chlorine dioxide but not from hydrogen peroxide. These findings highlight the importance of drinking water quality for effective vaccination. Full article

The compatibility of economic efficiency and animal welfare is a major challenge given the increasing demand for animal-based foods. Various studies have shown that it is possible to promote the resistance and robustness of commercial poultry (primarily broilers) by modifying incubation temperatures. Focused on the histology of the Bursa fabricii, an important lymphatic organ in birds, the investigations in this paper aim to show whether short-term temperature changes during final incubation (+1 °C, 2 h/incubation day 17–20) could have an influence on the robustness of broilers compared to conventionally incubated ones. Overall, however, the temperature stimulation during final embryonic development did not result in any statistically significant morphological changes in the bursa or in the heterophil-to-lymphocyte ratio (HLR) that would clearly indicate improved immune function. However, there are obvious sex-specific differences. For instance, a sexual dimorphism could be seen in the parameters of follicle number, follicle density and in the HLR when looking at the absolute numbers. Calculation of the effect size using Cohen’s d showed that there was an effect on the relative weight of the Bursa fabricii (d = −0.28, d ♂ = −0.25, d ♀ = −0.35) cell density (d ♀ = −0.32), follicle density (d ♀ = 0.37) and the HLR (d = 0.24, d ♂ = 0.43), so that further investigations should be encouraged. Full article