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BioChem, Volume 4, Issue 1

March 2024 - 3 articles

Cover Story: Muscle lactate dehydrogenase (LDH-A) catalyzes the reduction in pyruvate to lactate, the end product of anaerobic glycolysis. LDH-A is overexpressed in many cancers prior to and even when tumors receive adequate oxygen, and lactate has multiple cellular roles. Singlet oxygen and hypochlorous acid (HOCl) induced distinct patterns of protein crosslinks in LDH-A that were observed using SDS-PAGE under reducing conditions. LDH-A cysteines were oxidized via HOCl (only 1–10 equivalents over enzyme) but were resistant to H₂O₂, chloramines, and Angeli’s salt. HOCl oxidation inhibited LDH-A activity and yielded inter-chain disulfides observed using nonreducing SDS-PAGE. However, disulfide reduction did not restore LDH-A activity, which was lost due to HOCl oxidation. An irreversible conformational change induced via HOCl was detected using native gel electrophoresis and tryptophan fluorescence. View this paper

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Review

32 Citations

6,725 Views

24 Pages

Flavonoids and Flavonoid-Based Nanoparticles for Osteoarthritis and Rheumatoid Arthritis Management

Hicham Wahnou,
Youness Limami and
Mounia Oudghiri

BioChem2024, 4(1), 38-61;https://doi.org/10.3390/biochem4010003

13 March 2024

Arthritis, a global health burden comprising osteoarthritis and rheumatoid arthritis, demands advanced therapeutic approaches. In this context, flavonoids, a diverse group of naturally occurring compounds abundant in fruits, vegetables, and medicinal...

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Article

3 Citations

4,704 Views

20 Pages

Inhibition of Lactate Dehydrogenase-A by Singlet Oxygen and Hypochlorous Acid via Cysteine Oxidation and Irreversible Conformational Changes

Lisa Landino,
Lydia Boike and
Taylor Lain

BioChem2024, 4(1), 18-37;https://doi.org/10.3390/biochem4010002

2 February 2024

Muscle lactate dehydrogenase (LDH-A) catalyzes the reduction of pyruvate to lactate, the end product of anaerobic glycolysis. LDH-A is overexpressed in many cancers prior to and even when tumors receive adequate oxygen, and lactate has multiple cellu...

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Article

2,867 Views

17 Pages

Electrospun Polyurethane Vascular Grafts for Cerebral Revascularization: A Pilot Study on Rats

Evelynn Vergauwen,
Michiel R. L. Tubeeckx,
Annemie Houben,
Sandra Van Vlierberghe,
Marc Demolder,
Guido R. Y. De Meyer,
Patrick Pauwels and
Tomas Menovsky

BioChem2024, 4(1), 1-17;https://doi.org/10.3390/biochem4010001

5 January 2024

The current standard technique for vascular grafting in cerebral revascularization surgery employs the interposition of an autologous blood vessel. Technical complications have necessitated the development of a synthetic alternative, but classical bi...

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BioChem - ISSN 2673-6411

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