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BioTech, Volume 14, Issue 3

2025 September - 26 articles

Cover Story: We present a comprehensive single-nucleus RNA sequencing (snRNA-seq) atlas of the adult mouse lumbar–lower thoracic spinal cord, integrating harmonized data from six studies and profiling over 80,000 nuclei. Unlike previous atlases, our approach incorporates both protein-coding genes and the full spectrum of non-coding RNAs (ncRNAs). Our findings reveal that ncRNAs provide robust, cell-type-specific transcriptional signatures that significantly enhance clustering resolution and improve discrimination of functionally distinct neuronal populations. View this paper
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Articles (26)

  • Review
  • Open Access
1 Citations
2,039 Views
20 Pages

22 September 2025

Breast cancer is the most prevalent cancer among women and is challenging to diagnose and treat due to its diverse subtypes and stages. Precision medicine aims to improve early detection, prognosis, and treatment planning by identifying new clinical...

  • Review
  • Open Access
1 Citations
2,228 Views
29 Pages

Bacillus Pectinases as Key Biocatalysts for a Circular Bioeconomy: From Green Extraction to Process Optimization and Industrial Scale-Up

  • Fatima Zohra Kaissar,
  • Khelifa Bouacem,
  • Mohammed Lamine Benine,
  • Sondes Mechri,
  • Shubha Rani Sharma,
  • Vishal Kumar Singh,
  • Mahfoud Bakli,
  • Seif El Islam Lebouachera and
  • Giovanni Emiliani

19 September 2025

Pectins are high-value plant cell-wall polysaccharides with extensive applications in the food, pharmaceutical, textile, paper, and environmental sectors. Traditional extraction and processing methodologies rely heavily on harsh acids, high temperatu...

  • Article
  • Open Access
1 Citations
1,137 Views
13 Pages

12 September 2025

Models that predict the 3D structure of proteins enable us to easily analyze the structure of unknown proteins. Though many of these models have been found to be accurate, their application in plant proteins is not always entirely accurate. Thus, we...

  • Article
  • Open Access
835 Views
24 Pages

12 September 2025

While databases are emerging across various domains, from small molecules to genomics and proteins, aptamer databases remain scarce, if not entirely absent. Such databases could serve as a comprehensive resource for advancing research, innovation, an...

  • Article
  • Open Access
1,218 Views
21 Pages

Dual Role of Tenebrio molitor Frass in Sustainable Agriculture: Effects on Free-Living Nematodes and Suppression of Meloidogyne incognita

  • Evgenia Rizou,
  • Nikolaos Monokrousos,
  • Triantafyllia Kardami,
  • Georgia V. Baliota,
  • Christos I. Rumbos,
  • Christos G. Athanassiou,
  • Nikolaos Tsiropoulos and
  • Nikoletta Ntalli

8 September 2025

Insect-derived frass is gaining attention as a circular bioeconomy product with fertilizing and pest-suppressive potential. This study investigates Tenebrio molitor frass as a soil amendment for promoting beneficial nematodes and suppressing Meloidog...

  • Article
  • Open Access
2,118 Views
23 Pages

A Single-Nucleus Transcriptomic Atlas of the Mouse Lumbar Spinal Cord: Functional Implications of Non-Coding RNAs

  • Pablo Ruiz-Amezcua,
  • Miguel Nieto Hernández,
  • Javier García Flores,
  • Clara Plaza Alonso,
  • David Reigada,
  • Teresa Muñoz-Galdeano,
  • Eva Vargas,
  • Rodrigo M. Maza,
  • Francisco J. Esteban and
  • Manuel Nieto-Díaz

3 September 2025

The adult lumbar spinal cord plays a critical role in locomotor control and somatosensory integration, whose transcriptional architecture under physiological conditions has been characterized in various studies with restricted numbers of individuals...

  • Article
  • Open Access
2,184 Views
13 Pages

Effect of Selected Truffle-Associated Bacteria and Fungi on the Mycorrhization of Quercus ilex Seedlings with Tuber melanosporum

  • Eva Gómez-Molina,
  • Pedro Marco,
  • Sergi Garcia-Barreda,
  • Vicente González and
  • Sergio Sánchez

1 September 2025

The success of truffle cultivation is especially dependent on the quality of truffle-mycorrhized seedlings, which are typically produced in nurseries under aseptic conditions to avoid root colonization by undesired ectomycorrhizal fungi. However, suc...

  • Article
  • Open Access
1,833 Views
18 Pages

Organic Acid Production by Basfia succiniciproducens from Agro-Industrial By-Products

  • Márta Balázs,
  • Izabella Péter,
  • Hunor Bartos,
  • Zsolt Bodor,
  • Emőke Antal,
  • Csilla Albert and
  • Ildikó Miklóssy

1 September 2025

A continuous effort is needed to develop sustainable production methods for industrial platform chemicals. B. succiniciproducens, a natural succinic acid-producer, can metabolize five and six carbon atoms containing sugars in pure form as well as fro...

  • Article
  • Open Access
1,094 Views
20 Pages

28 August 2025

Extremophilic biological process (EBP) pretreatment increases substrate availability in anaerobic digestion, but the effect on downstream microbial community composition in industrial systems is not characterized. Changes in microbial communities wer...

  • Article
  • Open Access
1,586 Views
18 Pages

Enhanced Bacterial Cellulose Production Using Hempseed Meal: Optimal Conditions and Properties

  • Sawichaya Orpool,
  • Suthaphat Kamthai,
  • Thanyaporn Siriwoharn,
  • Patompong Khaw-on,
  • Aree Deenu and
  • Srisuwan Naruenartwongsakul

27 August 2025

Hemp (Cannabis sativa L.) seed is progressively emerging as an innovative and sustainable source of plant oil. Defatted hempseed meal is rich in protein and carbohydrates, which bacteria can convert into cellulose using glucose and fructose. The opti...

  • Review
  • Open Access
2,873 Views
12 Pages

26 August 2025

Neurosurgery is undergoing a significant transformation driven by advances in biomaterials and tissue engineering. These interdisciplinary innovations address challenges in repairing and regenerating neural tissues, integrating cranial and spinal imp...

  • Article
  • Open Access
1,160 Views
10 Pages

Translocation of Insecticidal Bt Protein in Transgrafted Plants

  • Arisa Ando,
  • Hitomi Ohkubo,
  • Hisae Maki,
  • Takumi Nishiuchi,
  • Takumi Ogawa,
  • Tomofumi Mochizuki,
  • Daisaku Ohta,
  • Hiroaki Kodama and
  • Taira Miyahara

25 August 2025

Transgrafting constitutes a technique involving the integration of genetically modified (GM) and non-GM plant organisms. Typically, edible components derived from non-GM scions are categorized as non-GM food products, attributed to the absence of exo...

  • Article
  • Open Access
2,464 Views
15 Pages

Enhancing In Vitro Regeneration in Three Sweet Potato Genotypes: Interplay Between Disinfectant, Explant Age, and Genotype

  • El Hadj Hussein Tapily,
  • Kan Modeste Kouassi,
  • Marius Konan Kouassi,
  • John Steven S. Seka,
  • Fidèle Tiendrébéogo and
  • Justin S. Pita

19 August 2025

Regenerating sweet potato from field-derived plant material requires careful management of several critical factors, including the effectiveness of the disinfectant, the age of the explant, and the genotype used. In this context, establishing a relia...

  • Article
  • Open Access
898 Views
16 Pages

Effects of Glycerol and Phenolics on Myceliophthora heterothallica Endoxylanase Expressed in K. phaffii

  • Jéssica de Araujo Zanoni,
  • Izabela Karolina Costa Zilli,
  • Guilherme de Paula Pretto,
  • Flavio Augusto Vicente Seixas,
  • Marcela Marques de Freitas Lima,
  • Eliana Gertrudes de Macedo Lemos,
  • Eleni Gomes,
  • Gabriel Zazeri and
  • Gustavo Orlando Bonilla-Rodriguez

18 August 2025

Industrial applications of xylanases in high-temperature settings are limited by enzyme instability. This study evaluated glycerol and phenolic compounds as modulators of the catalytic and structural properties of a recombinant Myceliophthora heterot...

  • Article
  • Open Access
1,800 Views
18 Pages

Rosemary Extract: Phytochemical Composition and Potential for Eliminating Polymicrobial Biofilm of Candida albicans and Multidrug-Resistant Bacteria

  • Tuana Mendonça Faria Cintra,
  • Raquel Teles de Menezes,
  • Lara Steffany de Carvalho,
  • Leticia de Miguel Nazario,
  • Leandro Wang Hantao,
  • Maria Cristina Marcucci,
  • Luciane Dias de Oliveira and
  • Vanessa Marques Meccatti-Domiciano

13 August 2025

Herbal medicines can be promising for the treatment of infections caused by multidrug-resistant microorganisms. This study aimed to evaluate Rosmarinus officinalis (Rosemary) hydroalcoholic extract (RHE) regarding its phytochemical composition and po...

  • Article
  • Open Access
4 Citations
1,758 Views
20 Pages

11 August 2025

The study of microalgae has led to significant progress in recent decades. The current microalgal biomass yield is unsatisfactory, except for certain species that are cultivated for the nutraceutical and pharmaceutical industries. In this study, the...

  • Article
  • Open Access
1 Citations
2,436 Views
12 Pages

Effect of Ultrasonic Pretreatment on the Extraction Process of Essential Oils from Grapefruit (Citrus paradisi) By-Products

  • Francisco Cadena-Cadena,
  • Joe Luis Arias-Moscoso,
  • Leandris Argentel-Martínez,
  • Jony R. Torres Velazquez,
  • Dulce Alondra Cuevas-Acuña,
  • Nydia Estrellita Buitimea Cantua and
  • Bartolo Concha-Frías

7 August 2025

This study evaluated the effect of ultrasonic pulse-assisted extraction on the yield and antioxidant activity of essential oils from grapefruit (Citrus paradisi) by-products using hydrodistillation and Soxhlet solvent extraction (hexane, acetone, eth...

  • Review
  • Open Access
2 Citations
3,609 Views
21 Pages

Pathway Analysis Interpretation in the Multi-Omic Era

  • William G. Ryan V.,
  • Smita Sahay,
  • John Vergis,
  • Corey Weistuch,
  • Jarek Meller and
  • Robert E. McCullumsmith

29 July 2025

In bioinformatics, pathway analyses are used to interpret biological data by mapping measured molecules with known pathways to discover their functional processes and relationships. Pathway analysis has become an essential tool for interpreting large...

  • Article
  • Open Access
1 Citations
1,180 Views
23 Pages

Fetal Health Diagnosis Based on Adaptive Dynamic Weighting with Main-Auxiliary Correction Network

  • Haiyan Wang,
  • Yanxing Yin,
  • Liu Wang,
  • Yifan Wang,
  • Xiaotong Liu and
  • Lijuan Shi

28 July 2025

Maternal and child health during pregnancy is an important issue in global public health, and the classification accuracy of fetal cardiotocography (CTG), as a key tool for monitoring fetal health during pregnancy, is directly related to the effectiv...

  • Article
  • Open Access
1,529 Views
16 Pages

Impact of Cattle Breed in scRNA-Seq Reference on Muscle Fiber Type Deconvolution from Bulk RNA-Seq: A Comparison of Software Tools

  • Raphael P. Moreira,
  • Marcelo R. Vicari,
  • Henrique A. Mulim,
  • Theresa M. Casey,
  • Jacquelyn Boerman,
  • Xing Fu and
  • Hinayah R. Oliveira

25 July 2025

While bulk RNA sequencing provides a comprehensive view of transcriptomes, it lacks cell type specificity. Single-cell RNA sequencing (scRNA-seq) overcomes this limitation by providing detailed insights at the individual cell level, though it involve...

  • Article
  • Open Access
1 Citations
2,226 Views
14 Pages

The Role of Microarray in Modern Sequencing: Statistical Approach Matters in a Comparison Between Microarray and RNA-Seq

  • Isaac D. Raplee,
  • Samiksha A. Borkar,
  • Li Yin,
  • Guglielmo M. Venturi,
  • Jerry Shen,
  • Kai-Fen Chang,
  • Upasana Nepal,
  • John W. Sleasman and
  • Maureen M. Goodenow

Gene expression analysis is crucial in understanding cellular processes, development, health, and disease. With RNA-seq outpacing microarray as the chosen platform for gene expression, is there space for array data in future profiling? This study inv...

  • Article
  • Open Access
1,459 Views
25 Pages

Large-scale bioprocesses often suffer from spatial heterogeneities, which impact microbial performance and often lead to phenotypic population heterogeneity. To better understand these effects at the single-cell level, this study applied, for the fir...

  • Communication
  • Open Access
1,109 Views
11 Pages

This study investigates the cytotoxic properties of metal complexes incorporating thio-uracil derivatives, specifically 2,4-dithiouracil and 6-propyl-2-thiouracil. The research focuses on the cytotoxic effects of Cu(II) and Pd(II) complexes with 6-pr...

  • Article
  • Open Access
2,229 Views
13 Pages

23 June 2025

Fibronectin (FN), a primary component of the extracellular matrix (ECM), features multiple structural domains closely linked to various cellular behaviors, including migration, spreading, adhesion, and proliferation. The FN3 domain, which contains th...

  • Article
  • Open Access
1,804 Views
13 Pages

In Silico Identification of Pathogenicity Effectors on Fusarium oxysporum f. sp. vanillae

  • Felipe Roberto Flores-de la Rosa,
  • Cristian Matilde-Hernández,
  • Nelly Abigail González-Oviedo,
  • Humberto José Estrella-Maldonado,
  • Liliana Eunice Saucedo-Picazo and
  • Ricardo Santillán-Mendoza

20 June 2025

Vanilla is a highly valuable spice in multiple industries worldwide. However, it faces a serious problem due to a disease known as root and stem rot, caused by the fungus Fusarium oxysporum f. sp. vanillae. Little is known about the pathogenicity mec...

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BioTech - ISSN 2673-6284