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Proceeding Paper

Novel Coatings Based on Nanostructured Cefepime-Functionalized Magnetite for Implantable Devices †

1
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
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Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
3
Research Institute of the University of Bucharest—ICUB, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
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National Institute for Lasers, Plasma and Radiation Physics, 077125 Măgurele, Romania
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Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Presented at the 2nd Coatings and Interfaces Web Conference, 15–31 May 2020; Available online: https://ciwc2020.sciforum.net/.
Mater. Proc. 2020, 2(1), 19; https://doi.org/10.3390/CIWC2020-06817
Published: 8 May 2020
(This article belongs to the Proceedings of 2nd Coatings and Interfaces Web Conference (CIWC-2 2020))
The aim of this study was to obtain biocompatible coatings based on polylactic acid, hydroxyapatite and nanostructured Cefepime-functionalized magnetite for enhancing the activity of next-generation implants against antibiotic-resistant pathogens. Mixtures of various ratios of polylactic acid, hydroxyapatite and nanostructured Cefepime-functionalized magnetite (Fe3O4@CEF, HAP/Fe3O4@CEF and PLA/Fe3O4@CEF) were obtained and deposited on glass slides by Matrix Assisted Pulsed Laser Evaporation (MAPLE). The in vitro biological effects of these coated surfaces on murine normal osteoblasts (MC3T3-E1 Subclone 4 (ATCC cat. no. CRL-2593)) were investigated by observing their morphological features and measuring the cell viability and nitric oxide (NO) release as an indicator of inflammation and cell death. A good biocompatibility was noticed for all samples investigated within this study, according to a formazan-based assay. Additionally, no increase in NO level was induced after 24 h of cell growth on these coated glass slides. Moreover, the visible microscopy images showed a good cell attachment on these modified surfaces and proved that the proliferative capacity of ostelasts was not disturbed in the presence of tested samples. The coatings succeeded in reducing the microbial attachment as well as the subsequent Escherichia coli colonization and biofilm development on these surfaces. In conclusion, these novel coatings can become suitable surfaces for implantable devices with an enhanced biocompatibility and reduced bacterial colonization.
Keywords: coatings; polylactic acid; hydroxyapatite; cefepime; magnetite; osteoblasts coatings; polylactic acid; hydroxyapatite; cefepime; magnetite; osteoblasts
MDPI and ACS Style

Stan, M.S.; Nica, I.C.; Dinischiotu, A.; Grumezescu, V.; Stoica, A.E.; Holban, A.M.; Grumezescu, A. Novel Coatings Based on Nanostructured Cefepime-Functionalized Magnetite for Implantable Devices. Mater. Proc. 2020, 2, 19. https://doi.org/10.3390/CIWC2020-06817

AMA Style

Stan MS, Nica IC, Dinischiotu A, Grumezescu V, Stoica AE, Holban AM, Grumezescu A. Novel Coatings Based on Nanostructured Cefepime-Functionalized Magnetite for Implantable Devices. Materials Proceedings. 2020; 2(1):19. https://doi.org/10.3390/CIWC2020-06817

Chicago/Turabian Style

Stan, Miruna S., Ionela Cristina Nica, Anca Dinischiotu, Valentina Grumezescu, Alexandra Elena Stoica, Alina Maria Holban, and Alexandru Grumezescu. 2020. "Novel Coatings Based on Nanostructured Cefepime-Functionalized Magnetite for Implantable Devices" Materials Proceedings 2, no. 1: 19. https://doi.org/10.3390/CIWC2020-06817

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