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Accuracy of Subclinical Ketosis Detection with Rapid Test Methods for BHBA in Blood in Commercial Dairy Farms

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Division of Ruminant Nutrition, Department of Animal Sciences, University of Goettingen, Kellnerweg 6, 37077 Goettingen, Germany
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Department 3.5, Research Livestock, Chamber of Agriculture Lower Saxony, Mars-la-Tour-Straße 1-6, 26121 Oldenburg, Germany
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Division of Biotechnology and Reproduction of Livestock, Department of Animal Sciences, University of Goettingen, Burckhardtweg 2, 37077 Goettingen, Germany
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Division of Animal Breeding and Genetics, Department of Animal Sciences, University of Goettingen, Albrecht-Thaer-Weg 3, 37075 Goettingen, Germany
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Authors to whom correspondence should be addressed.
Academic Editors: Abderrahmane Ait Kaddour and Sandra Abreu
Dairy 2021, 2(4), 671-683; https://doi.org/10.3390/dairy2040052 (registering DOI)
Received: 5 October 2021 / Revised: 9 November 2021 / Accepted: 18 November 2021 / Published: 25 November 2021
This study gives an overview of the performance and accuracy of devices used for the fast measurement of β-hydroxybutyrate (BHBA) in blood for the on-farm indication of subclinical ketosis. Data were collected on ten dairy farms. In each farm, blood samples were taken from ten cows on four test days (2, 4, 9 and 11), resulting in 400 samples. The reference method was the BHBA concentration in blood serum (BHBALAB). Four different devices that measure BHBA in whole blood were tested. The thresholds applied for identifying subclinical ketosis were ≥1.0, ≥1.2 and ≥1.4 mmol/L in blood serum. The BHBALAB was assigned in three classes: low—≤0.9 mmol/L; high—>0.9 mmol/L; and total—all values unclassified. Due to initial negative effects on the health and performance of cows with BHBA levels ≥0.9 mmol/L, this cut-off was chosen. The Passing–Bablok regression revealed different constant as well as absolute biases for each device in the aforementioned classes. The area under the receiver operating characteristics curve indicated highly accurate results, with 94–97% accuracy levels. As an overall conclusion, the performance of the devices was good and supports their use by farmers for the detection of subclinical ketotic cows in their herds. View Full-Text
Keywords: hyperketonemia; ketone bodies; milk production; β-hydroxybutyrate; diagnostic test; dairy cow; on-farm; rapid test hyperketonemia; ketone bodies; milk production; β-hydroxybutyrate; diagnostic test; dairy cow; on-farm; rapid test
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MDPI and ACS Style

Jansen, H.M.; Zschiesche, M.; Albers, D.; Wemheuer, W.; Sharifi, A.R.; Hummel, J. Accuracy of Subclinical Ketosis Detection with Rapid Test Methods for BHBA in Blood in Commercial Dairy Farms. Dairy 2021, 2, 671-683. https://doi.org/10.3390/dairy2040052

AMA Style

Jansen HM, Zschiesche M, Albers D, Wemheuer W, Sharifi AR, Hummel J. Accuracy of Subclinical Ketosis Detection with Rapid Test Methods for BHBA in Blood in Commercial Dairy Farms. Dairy. 2021; 2(4):671-683. https://doi.org/10.3390/dairy2040052

Chicago/Turabian Style

Jansen, Henrike M., Marleen Zschiesche, Dirk Albers, Wilhelm Wemheuer, A. R. Sharifi, and Jürgen Hummel. 2021. "Accuracy of Subclinical Ketosis Detection with Rapid Test Methods for BHBA in Blood in Commercial Dairy Farms" Dairy 2, no. 4: 671-683. https://doi.org/10.3390/dairy2040052

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