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Methods and Protocols, Volume 7, Issue 1

February 2024 - 17 articles

Cover Story: High-grade glioblastoma multiforme is the deadliest brain tumor that is not yet curable. A new approach targeting glioblastoma cells is to produce pseudotyped viral particles that use the neurotropic properties of the Zika virus. The generated viral pseudotype is a particle with the inner core of a lentivirus such as HIVgfp (env-) coated with the envelope proteins of Zika virus. In this protocol, we describe the generation of a ZIKV/HIV-1 lentiviral vector with a 2-plasmid system using a nef+, gfp+, env- HIV plasmid and a second plasmid for Zika virus protein E, which was linked to the HIV-1 gp41 transmembrane domain. The functional number of pseudotype particles generated was significantly increased compared to previously published protocols. View this paper
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Articles (17)

  • Article
  • Open Access
5 Citations
4,407 Views
12 Pages

Mapping m6A Sites on HIV-1 RNA Using Oligonucleotide LC-MS/MS

  • Alice Baek,
  • Asif Rayhan,
  • Ga-Eun Lee,
  • Sarah Golconda,
  • Hannah Yu,
  • Shihyoung Kim,
  • Patrick A. Limbach,
  • Balasubrahmanyam Addepalli and
  • Sanggu Kim

The biological significance of chemical modifications to the ribonucleic acid (RNA) of human immunodeficiency virus type-1 (HIV-1) has been recognized. However, our understanding of the site-specific and context-dependent roles of these chemical modi...

  • Article
  • Open Access
7 Citations
3,624 Views
18 Pages

Wastewater-based epidemiology (WBE) has great potential to monitor community public health, especially during pandemics. However, it faces substantial hurdles in pathogen surveillance through WBE, encompassing data representativeness, spatiotemporal...

  • Study Protocol
  • Open Access
9 Citations
5,541 Views
12 Pages

Introducing Artificial Intelligence in Interpretation of Foetal Cardiotocography: Medical Dataset Curation and Preliminary Coding—An Interdisciplinary Project

  • Jasmin Leonie Aeberhard,
  • Anda-Petronela Radan,
  • Ramin Abolfazl Soltani,
  • Karin Maya Strahm,
  • Sophie Schneider,
  • Adriana Carrié,
  • Mathieu Lemay,
  • Jens Krauss,
  • Ricard Delgado-Gonzalo and
  • Daniel Surbek

Artificial intelligence (AI) is gaining increasing interest in the field of medicine because of its capacity to process big data and pattern recognition. Cardiotocography (CTG) is widely used for the assessment of foetal well-being and uterine contra...

  • Study Protocol
  • Open Access
2 Citations
3,066 Views
8 Pages

Introduction: Tuberculosis (TB) and Human Immunodeficiency Virus (HIV) remain major public health issues in sub-Saharan Africa. The co-occurrence of these diseases is a growing concern in the region, and social determinants, the circumstances under w...

  • Protocol
  • Open Access
1 Citations
3,514 Views
14 Pages

Generation of Viral Particles with Brain Cell-Specific Tropism by Pseudotyping HIV-1 with the Zika Virus E Protein

  • Hai Dang Ngo,
  • Jan Patrick Formanski,
  • Vivien Grunwald,
  • Birco Schwalbe and
  • Michael Schreiber

28 December 2023

Flaviviruses are a family of RNA viruses that includes many known pathogens, such as Zika virus (ZIKV), West Nile virus (WNV), dengue virus (DENV), and yellow fever virus (YFV). A pseudotype is an artificial virus particle created in vitro by incorpo...

  • Article
  • Open Access
2,990 Views
18 Pages

Nesting Ecology of Lepidochelys olivacea in Lobito, Angola

  • Inês M. Ferreira,
  • Luz Murillo,
  • Jean-Marie Le-Corre,
  • Marco Correia,
  • Rita Anastácio and
  • Mário J. Pereira

27 December 2023

The scarcity on the Atlantic coast of the African sea turtle population and its dynamics data is well known. This article discusses the nesting ecology methods and analysis of a nascent Angolan project aimed at preserving the nesting female populatio...

  • Communication
  • Open Access
2 Citations
3,152 Views
14 Pages

Valorizing Tree-Nutshell Particles as Delivery Vehicles for a Natural Herbicide

  • Jong H. Kim,
  • Kathleen L. Chan,
  • William M. Hart-Cooper,
  • DeAngela Ford,
  • Kaydren Orcutt,
  • Jeffrey D. Palumbo,
  • Christina C. Tam and
  • William J. Orts

20 December 2023

The United States is a principal producer of tree nuts (almonds, pistachios, and walnuts), resulting in the generation of excess of tree-nutshell by-products each year, with few market outlets. A nutshell is an essential, lignocellulosic layer that p...

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Methods Protoc. - ISSN 2409-9279