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Methods and Protocols, Volume 7, Issue 1

February 2024 - 17 articles

Cover Story: High-grade glioblastoma multiforme is the deadliest brain tumor that is not yet curable. A new approach targeting glioblastoma cells is to produce pseudotyped viral particles that use the neurotropic properties of the Zika virus. The generated viral pseudotype is a particle with the inner core of a lentivirus such as HIVgfp (env-) coated with the envelope proteins of Zika virus. In this protocol, we describe the generation of a ZIKV/HIV-1 lentiviral vector with a 2-plasmid system using a nef+, gfp+, env- HIV plasmid and a second plasmid for Zika virus protein E, which was linked to the HIV-1 gp41 transmembrane domain. The functional number of pseudotype particles generated was significantly increased compared to previously published protocols. View this paper
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Articles (17)

  • Protocol
  • Open Access
10 Citations
3,389 Views
21 Pages

Crafting a Rigorous, Clinically Relevant Large Animal Model of Chronic Myocardial Ischemia: What Have We Learned in 20 Years?

  • Christopher R. Stone,
  • Dwight D. Harris,
  • Mark Broadwin,
  • Meghamsh Kanuparthy,
  • Sharif A. Sabe,
  • Cynthia Xu,
  • Jun Feng,
  • M. Ruhul Abid and
  • Frank W. Sellke

19 February 2024

The past several decades have borne witness to several breakthroughs and paradigm shifts within the field of cardiovascular medicine, but one component that has remained constant throughout this time is the need for accurate animal models for the ref...

  • Article
  • Open Access
4 Citations
5,051 Views
22 Pages

Assessment of the Integrity and Function of Human Term Placental Explants in Short-Term Culture

  • Carolina López-Guzmán,
  • Ana María García,
  • Paula Marín and
  • Ana María Vásquez

15 February 2024

Human placental explants (HPEs) culture has generated significant interest as a valuable in vitro model for studying tissue functions in response to adverse conditions, such as fluctuations in oxygen levels, nutrient availability, exposure to pathoge...

  • Study Protocol
  • Open Access
2 Citations
4,049 Views
12 Pages

Pilot Study: Safety and Performance Validation of an Ingestible Medical Device for Collecting Small Intestinal Liquid in Healthy Volunteers

  • Alexandre Tronel,
  • Anne-Sophie Silvent,
  • Elena Buelow,
  • Joris Giai,
  • Corentin Leroy,
  • Marion Proust,
  • Donald Martin,
  • Audrey Le Gouellec,
  • Thomas Soranzo and
  • Nicolas Mathieu

4 February 2024

The connection between imbalances in the human gut microbiota, known as dysbiosis, and various diseases has been well established. Current techniques for sampling the small intestine are both invasive for patients and costly for healthcare facilities...

  • Article
  • Open Access
2 Citations
3,101 Views
10 Pages

Correlation between Tooth Position Parameters and Apical Fenestration: A Cone-Beam Computed Tomography Study

  • Carlos Henrique Ferrari,
  • Lara Steffany de Carvalho,
  • Caroline Trefiglio Rocha and
  • Amjad Abu Hasna

2 February 2024

This study aimed to assess the relationship between apical fenestration—a defect in the alveolar bone involving the root apex—and tooth position in all tooth groups, excluding the third molars, utilizing cone-beam computed tomography (CBC...

  • Protocol
  • Open Access
2 Citations
11,345 Views
31 Pages

1 February 2024

The development of monoclonal antibodies (mAbs) represents a significant milestone in both basic research and clinical applications due to their target specificity and versatility in therapeutic and diagnostic applications. The innovative strategy of...

  • Article
  • Open Access
4 Citations
3,654 Views
13 Pages

26 January 2024

The rapid advancement of genetic technologies has made it possible to modify various plants through both genetic transformation and gene editing techniques. Poplar, with its rapid in vitro growth and regeneration enabling high rates of micropropagati...

  • Technical Note
  • Open Access
2 Citations
8,099 Views
10 Pages

25 January 2024

Knowledge of the geometric quantities of the erythrocyte is useful in several physiological studies, both for zoologists and veterinarians. While the diameter and volume (MCV) are easily obtained from observations of blood smears and complete blood c...

  • Article
  • Open Access
2 Citations
3,644 Views
12 Pages

23 January 2024

Ostarine is frequently misused as a selective androgen receptor modulator (SARM) in sports. Consequently, there is a pressing need for reliable and simple approaches to monitor its presence in biological systems. In this work, we developed a two-dime...

  • Protocol
  • Open Access
6 Citations
3,148 Views
12 Pages

Spinal muscular atrophy is a neuromuscular disorder caused by mutations in both copies of the survival motor neuron gene 1 (SMN1), which lead to reduction in the production of the SMN protein. Currently, there are several therapies that have been app...

  • Protocol
  • Open Access
4 Citations
4,229 Views
10 Pages

Lateral flow immunochromatographic (LFI) tests are widely used in both biomedical and forensic sciences for different applications. In forensic sciences, their main use is to detect body fluids at crime scenes. However, there are situations in which...

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Methods Protoc. - ISSN 2409-9279