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Methods and Protocols, Volume 4, Issue 1

March 2021 - 23 articles

Cover Story: Bombyx mori is a model organism for lepidopteran genetics which is economically important for producing silk and is used in the textile, biomedical, and cosmetics sectors. Silkworms can also act as genetically engineered bioreactors to produce recombinant proteins. CRISPR/Cas9 technology is an efficient method of generating heritable gene-specific DNA modifications. After CRISPR/Cas9 mutagenesis, silkworms must be molecularly screened to identify individuals carrying a suitable DNA modification for selective breeding. We describe an efficient screening workflow involving a heteroduplex assay and Sanger sequencing for the characterization of mutations, and the amplification refractory mutation system PCR for the subsequent detection of the modification, enabling the establishment of B. mori mutant lines stably inheriting specific mutations. View this paper.

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Article

5 Citations

3,919 Views

11 Pages

Predictive Quantitative Structure–Activity Relationship Modeling of the Antifungal and Antibiotic Properties of Triazolothiadiazine Compounds

Michael Appell,
David L. Compton and
Kervin O. Evans

Methods Protoc.2021, 4(1), 2;https://doi.org/10.3390/mps4010002

27 December 2020

Predictive models were developed using two-dimensional quantitative structure activity relationship (QSAR) methods coupled with B3LYP/6-311+G** density functional theory modeling that describe the antimicrobial properties of twenty-four triazolothiad...

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Protocol

10 Citations

5,476 Views

14 Pages

Purification and Characterization of Recombinant Expressed Apple Allergen Mal d 1

Julia A. H. Kaeswurm,
Bettina Nestl,
Sven M. Richter,
Max Emperle and
Maria Buchweitz

Methods Protoc.2021, 4(1), 3;https://doi.org/10.3390/mps4010003

27 December 2020

Mal d 1 is the primary apple allergen in northern Europe. To explain the differences in the allergenicity of apple varieties, it is essential to study its properties and interaction with other phytochemicals, which might modulate the allergenic poten...

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Review

175 Citations

17,231 Views

20 Pages

miRNA Targets: From Prediction Tools to Experimental Validation

Giulia Riolo,
Silvia Cantara,
Carlotta Marzocchi and
Claudia Ricci

Methods Protoc.2021, 4(1), 1;https://doi.org/10.3390/mps4010001

24 December 2020

MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression in both animals and plants. By pairing to microRNA responsive elements (mREs) on target mRNAs, miRNAs play gene-regulatory roles, producing remarkable changes in several physio...

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Methods Protoc. - ISSN 2409-9279

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